Version: 2 Revision Date: 2013-08-09
Product Data Sheet
Brilliant Violet 421™ anti-human CD24
Human peripheral blood lymphocytes
stained with CD19 APC and CD24
(clone ML5) Brilliant Violet 421™ (top)
or mouse IgG2a, κ Brilliant Violet 421
™ isotype control (bottom).
Catalog # / Size: 311121 / 25 tests
311122 / 100 tests
Clone: ML5
Isotype: Mouse IgG2a, κ
Workshop Number: V CD24.5
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon
Preparation: The antibody was purified by affinity chromatography and conjugated with
Brilliant Violet 421™ under optimal conditions. The solution is free of
unconjugated Brilliant Violet 421™ and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and
BSA (origin USA).
Storage: The antibody solution should be stored undiluted between 2°C and 8°C, and
protected from prolonged exposure to light. Do not freeze.
Applications:
Applications: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent
staining with flow cytometric analysis. For flow cytometric staining, the
suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of
whole blood. It is recommended that the reagent be titrated for optimal
performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard
bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™
is a trademark of Sirigen Group Ltd.
Application Notes: Additional reported applications (for the relevant formats) include:
immunofluorescence microscopy3.
Application References: 1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell
Differentiation Antigens. Oxford University Press. New York.
2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press.
New York.
3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Description: CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1,
Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic
cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24
crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
Related Products:Product Clone Application
Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC, ICFC
Cell Staining Buffer FC, ICC, ICFC
RBC Lysis Buffer (10X) FC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution) FC, ICC, ICFC
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