Version: 1 Revision Date: 2012-11-30
Product Data Sheet
FITC Rat IgG2b, κ Isotype Ctrl
Catalog # / Size: 400633 / 25 tests
400605 / 50 µg
400634 / 100 tests
400606 / 200 µg
Clone: RTK4530
Isotype: Rat IgG2b, κ
Immunogen: Trinitrophenol + KLH
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The
solution is free of unconjugated FITC.
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: test sizes: lot-specific; µg sizes: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to
light. Do not freeze.
Applications:
Applications: FC, ICFC - Quality tested
Recommended Usage: Each lot of this rat IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary
antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting,
immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™
purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.
400622) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified
antibody (Cat. No. 400644) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01
EU/µg).
Application References: 1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
2. Zeiser R, et al. 2007. Blood 109:2225.
3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
5. Yi H, et al. 2009. Blood 113:5819. PubMed
6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
Description: The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after
screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third
parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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