Version: 2 Revision Date: 2014-09-11
Product Data Sheet
FITC anti-mouse/human CD44
C57BL/6 splenocytes stained with
IM7 FITC
Human peripheral blood lymphocytes
stained with IM7 FITC
Catalog # / Size: 103005 / 50 µg
103021 / 25 tests
103022 / 100 tests
103006 / 500 µg
Clone: IM7
Isotype: Rat IgG2b, κ
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Reactivity: Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus,
Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig,
Porcine), Dog (Canine), Cat (Feline)
Preparation: The antibody was purified by affinity chromatography, and conjugated with
FITC under optimal conditions. The solution is free of unconjugated FITC.
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium
azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium
azide.
Concentration: test size: lot-specific; µg size: 0.5 mg/ml
Storage: The CD44 antibody solution should be stored undiluted between 2°C and
8°C, and protected from prolonged exposure to light. Do not freeze.
Applications:
Applications: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent
staining with flow cytometric analysis. Test size products are transitioning
from 20 µl to 5 µl per test. Please check your vial or your CoA to find the
suggested use of this reagent per million cells in 100 µl staining volume or
per 100 µl of whole blood. It is recommended that the reagent be titrated for
optimal performance for each application. Read more at
www.biolegend.com/testsize regarding the test size change.
Application Notes: Clone IM7 has been reported to recognize an epitope common to
alloantigens and all isoforms of CD4417,18 that is located between amino
acids 145 and 18620. Additional reported applications (for the relevant
formats) include: immunohistochemistry of acetone-fixed frozen sections and
formalin-fixed paraffin-embedded sections6,7, complement-mediated
cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The
LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered)
is recommended for functional assays (Cat. No. 103014). For highly sensitive
assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046)
with a lower endotoxin limit than standard LEAF™ purified antibodies
(Endotoxin <0.01 EU/µg).
Application References: 1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
8. Lee JW, et al. 2006. Nature Immunol. 8:181.
9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
12. Wang XY, et al. 2008. Blood 111:2436. PubMed
13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
14. Yamazaki J, et al. 2009. Blood PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third
parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
20. Sandmaier BM, et al. 1998. Blood 91:3494.
Description: CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes,
endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory
stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a
valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent
suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to
and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte
aggregation.
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third
parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587