1	
  
Structural	
  Virology	
  
Lecture	
  10	
  
Pavel	
  Plevka	
  
2	
  
Virus	
  vaccines	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
3	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
4	
  
AnEbody	
  dependent	
  enhancement	
  
dengue	
  
5	
  
AnEbody	
  dependent	
  enhancement	
  -­‐	
  
coronaviruses	
  
6	
  
Smallpox	
  
•  killed	
  an	
  esEmated	
  400,000	
  
Europeans	
  annually	
  during	
  
the	
  18th	
  century.	
  
•  was	
  responsible	
  for	
  a	
  third	
  of	
  
all	
  blindness.	
  
•  of	
  those	
  infected,	
  20–60%—
and	
  over	
  80%	
  of	
  infected	
  
children—died	
  
•  responsible	
  for	
  an	
  esEmated	
  
300–500	
  million	
  deaths	
  
during	
  the	
  20th	
  century	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
7	
  
Vaccine	
  Types	
  
•  AUenuated	
  viruses	
  
•  InacEvated	
  viruses	
  
•  Virion	
  subunits	
  
•  Recombinant	
  viruses	
  
•  Virus-­‐like	
  parEcles	
  
•  SyntheEc	
  pepEdes	
  
•  DNA	
  vaccines	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
8	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
9	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
10	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
11	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
12	
  
13	
  
Learning	
  outcomes	
  
•  explain	
  how	
  virus	
  diseases	
  are	
  controlled	
  with	
  
vaccines	
  
•  evaluate	
  the	
  types	
  of	
  virus	
  vaccine	
  in	
  medical	
  
and	
  veterinary	
  use	
  
•  evaluate	
  types	
  of	
  virus	
  vaccine	
  that	
  are	
  experi-­‐	
  
mental	
  
•  describe	
  methods	
  used	
  to	
  manufacture	
  virus	
  
vaccines	
  
•  evaluate	
  procedures	
  designed	
  to	
  ensure	
  the	
  
safety	
  of	
  virus	
  vaccines	
  
14	
  
AnEviral	
  compounds	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
15	
  
HIV	
   HCV	
  
16	
  
17	
  
18	
  
Picornavirus	
  virion	
  
Plevka	
  et	
  al.	
  Science	
  2012.	
  
1.	
  Genome	
  delivery	
  into	
  cytoplasm	
  
Rossmann	
  Prot	
  Sci,	
  1994	
  	
  
Pocket	
  factor	
  in	
  EV71	
  
Smith	
  TJ,	
  et	
  al.	
  Science,	
  1986	
  
InhibiEon	
  of	
  EV71	
  by	
  WIN	
  51711	
  
Plevka	
  et	
  al.	
  PNAS	
  2013	
  
28	
  
Learning	
  outcomes	
  
•  evaluate	
  procedures	
  used	
  to	
  develop	
  new	
  anE-­‐	
  
viral	
  drugs	
  
•  describe	
  the	
  modes	
  of	
  acEon	
  of	
  selected	
  anE-­‐
viral	
  drugs	
  
•  discuss	
  virus	
  resistance	
  to	
  drugs	
  
29	
  
Prions	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
30	
  
Brain	
  with	
  Scrapie	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
31	
  
Normal	
  and	
  misfolded	
  form	
  of	
  prion	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
32	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
33	
  
Creutzfeldt-­‐Jacob	
  disease	
  x	
  BSE	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
34	
  
Viroids	
  
©	
  2012	
  John	
  Wiley	
  &	
  Sons	
  Ltd.	
  
www.wiley.com/college/carter	
  
35	
  
Virophage	
  -­‐	
  Sputnik