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20181030PDKblaZ
Experiment
Creation Date	10/30/2018 12:47:55 PM	Last Modified Date	10/30/2018 3:12:32 PM
Operator	LMDM	Owner	LMDM
Start Time	10/30/2018 1:00:24 PM	End Time	10/30/2018 2:04:35 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	02122014blaZkvantifikace Run Protocol	Plate ID	04573905
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		stage 1							
Cycles			Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name Cycles		stage 2							
		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		stage 3							
Cycles		40	Analysis Mode | Quantification						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	Single		00:00:30	2.20		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
	95	Continuous			0.11	5	0	0	0
20181030PDKblaZ
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Page 1 of 8
Program Name		cooling							
Cycles		1	Analysis Mode		None				
	Target	Acquisition		Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode		(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
	40	None		00:01:00	2.20		0	0	0
Samples
Sample Count
Subset
96
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	plJB		A1	
A2	plJB		A1	
A3	plJB		A1	
A4	plJB		A4	
A5	plJB		A4	
A6	plJB		A4	
A7	Sample 7		A7	
A8	Sample 7		A7	
A9	Sample 7		A7	
A10	Sample 10		A10	
A11	Sample 10		A10	
A12	Sample 10		A10	
B1	Sample 13		B1	
B2	Sample 13		B1	
B3	Sample 13		B1	
B4	Sample 16		B4	
B5	Sample 16		B4	
B6	Sample 16		B4	
B7	Sample 19		B7	
B8	Sample 19		B7	
B9	Sample 19		B7	
B10	Sample 22			
B11	Sample 23			
B12	Sample 24			
C1	Tet1		C1	
C2	Tet1		C1	
C3	Tet1		C1	
C4	Cd1		C4	
C5	Cd1		C4	
C6	Cd1		C4	
C7	Cd2		C7	
C8	Cd2		C7	
C9	Cd2		C7	
20181030PDKblaZ
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Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C10	Cd3		C10	
C11	Cd3		C10	
C12	Cd3		C10	
D1	1		D1	
D2	1		D1	
D3	1		D1	
D4	3		D4	
D5	3		D4	
D6	3		D4	
D7	Sample 43		D7	
D8	Sample 43		D7	
D9	Sample 43		D7	
D10	Sample 46			
D11	Sample 47			
D12	Sample 48			
E1	Sample 49			
E2	Sample 50			
E3	Sample 51			
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
20181030PDKblaZ
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Page 3 of 8
Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
Subset Name
All Samples
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
20181030PDKblaZ
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Page 4 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	plJB	Standard	22.52	3.05E3	6.00E3	
0	A2	plJB	Standard	24.46	7.23E2	6.00E3	
0	A3	plJB	Standard	21.72	5.55E3	6.00E3	
0	A4	plJB	Standard	24.93	5.10E2	6.00E2	
0	A5	plJB	Standard	24.45	7.29E2	6.00E2	
0	A6	plJB	Standard	23.57	1.40E3	6.00E2	
0	A7	Sample 7	Standard	26.32	1.80E2	6.00E1	
0	A8	Sample 7	Standard	26.54	1.54E2	6.00E1	
0	A9	Sample 7	Standard	26.55	1.53E2	6.00E1	
0	A10	Sample 10	Standard	30.00	1.17E1	6.00E0	
0	A11	Sample 10	Standard	31.62	3.50E0	6.00E0	
0	A12	Sample 10	Standard	31.52	3.78E0	6.00E0	
0	B1	Sample 13	Standard	35.00	5.57E-2	6.00E-1	>
0	B2	Sample 13	Standard	32.83	1.13E0	6.00E-1	
0	B3	Sample 13	Standard	33.05	8.82E-1	6.00E-1	
0	B4	Sample 16	Standard	35.00	5.57E-2	6.00E-2	>
0	B5	Sample 16	Standard	35.00	5.57E-2	6.00E-2	>
0	B6	Sample 16	Standard	35.00	5.57E-2	6.00E-2	>
0	B7	Sample 19	Unknown				
0	B8	Sample 19	Unknown				
0	B9	Sample 19	Unknown				
0	B10	Sample 22	Unknown				
0	B11	Sample 23	Unknown				
0	B12	Sample 24	Unknown				
0	C1	Tet1	Unknown	35.00	5.57E-2		>, E
0	C2	Tet1	Unknown				
0	C3	Tet1	Unknown				
0	C4	Cd1	Unknown	28.07	4.93E1		
0	C5	Cd1	Unknown	28.05	5.00E1		
0	C6	Cd1	Unknown	27.96	5.36E1		
0	C7	Cd2	Unknown	28.75	2.97E1		
0	C8	Cd2	Unknown	28.52	3.53E1		
0	C9	Cd2	Unknown	27.97	5.29E1		
0	C10	Cd3	Unknown	26.59	1.48E2		
0	C11	Cd3	Unknown	23.84	1.14E3		
0	C12	Cd3	Unknown	26.92	1.15E2		
0	D1	1	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
20181030PDKblaZ
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	D2	1	Unknown				
0	D3	1	Unknown				
0	D4	3	Unknown	20.10	1.85E4		E
0	D5	3	Unknown	20.59	1.29E4		E
0	D6	3	Unknown	21.52	6.45E3		E
0	D7	Sample 43	Negative Control	35.00	5.57E-2		>
0	D8	Sample 43	Negative Control				
0	D9	Sample 43	Negative Control				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	Sample 49	Unknown				
0	E2	Sample 50	Unknown				
0	E3	Sample 51	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
20181030PDKblaZ
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Page 6 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone 2.42E3
A1, A2, A3	22.90	1.41	3.11E3	
A4, A5, A6	24.31	0.69	8.81 E2	4.66E2
A7, A8, A9	26.47	0.13	1.62E2	1.55E1
A10, A11, A12	31.05	0.91	6.32E0	4.65E0
B1, B2, B3	33.63	1.19	6.88E-1	5.61E-1
B4, B5, B6	35.00		5.57E-2	
B7, B8, B9				
C1, C2, C3	35.00		5.57E-2	
C4, C5, C6	28.02	0.06	5.10E1	2.28E0
C7, C8, C9	28.41	0.40	3.93E1	1.21E1
C10, C11, C12	25.79	1.69	4.69E2	5.84E2
D1, D2, D3				
20181030PDKblaZ
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Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
D4, D5, D6	20.74	0.72	1.26E4	6.04E3
D7, D8, D9	35.00		5.57E-2	
Amplification Curves
34.328; 31.32fr 28.32fr 25.32fr
* 22.32fr' in
I 19.32fr s 16.328
n
8 13.328
L.
*
I 10.32fr 7.328; 4.32fr 1.32fr -1.G72:
10
15
20
Cycles
25
30
35
40
Standard Curve
Error: 0.0958 Efficiency: 2.105 Slope: -3.094 Ylntercept: 33.30 Link: 3.779
Std. curve Samples
0.5 1 1J5
Log Concentration
2.5
Analysis Notes
20181030PDKblaZ
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