.1
J ■
LightCycler® 480 Software
' IlllllllliF
Report_ ■■■■■■■■■■bp
<Ročhe)
181211blaZ
Experiment
Creation Date	12/11/2018 12:16:39 PM	Last Modified Date	12/11/2018 3:23:55 PM
Operator	LMDM	Owner	LMDM
Start Time	12/11/2018 12:29:47 PM	End Time	12/11/2018 1:45:55 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	blaZ kvantifikace cviko	Plate ID	04573928
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		stage 1							
Cycles			Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name Cycles		stage 2							
		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		stage 3							
Cycles		40	Analysis Mode | Quantification						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	None		00:00:30	2.20		0	0	0
	72	Single		00:00:15	4.40		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
181211blaZ
12/11/2018
Page 1 of 9
	Target (°C)	Acquisition Mode	Hold (hh:mm:ss)	Ramp Rate CC/s)	Acquisitions (per'C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	Continuous		0.11	5	0	0	0
Program Name		cooling						
Cycles		1              Analysis Mode		None				
	Target (°C)	Acquisition Mode	Hold (hh:mm:ss)	- Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	40	None	00:01:00	2.20		0	0	0
Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	blaZ plJB		A1	
A2	blaZ plJB		A1	
A3	blaZ plJB		A1	
A4	Sample 4		A4	
A5	Sample 4		A4	
A6	Sample 4		A4	
A7	Sample 7		A7	
A8	Sample 7		A7	
A9	Sample 7		A7	
A10	Sample 10		A10	
A11	Sample 10		A10	
A12	Sample 10		A10	
B1	Sample 13		B1	
B2	Sample 13		B1	
B3	Sample 13		B1	
B4	Sample 16		B4	
B5	Sample 16		B4	
B6	Sample 16		B4	
B7	Sample 19			
B8	Sample 20			
B9	Sample 21			
B10	Sample 22			
B11	Sample 23			
B12	Sample 24			
C1	H20		C1	
C2	H20		C1	
C3	H20		C1	
C4	T1		C4	
C5	T1		C4	
C6	T1		C4	
181211blaZ
12/11/2018
Page 2 of 9
Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C7	T2		C7	
C8	T2		C7	
C9	T2		C7	
C10	T3		C10	
C11	T3		C10	
C12	T3		C10	
D1	T4		D1	
D2	T4		D1	
D3	T4		D1	
D4	T5		D4	
D5	T5		D4	
D6	T5		D4	
D7	T6		D7	
D8	T6		D7	
D9	T6		D7	
D10	T7		D10	
D11	T7		D10	
D12	T7		D10	
E1	Sample 49			
E2	Sample 50			
E3	Sample 51			
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
181211blaZ
12/11/2018
Page 3 of 9
Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Instrument
Name	29712	Serial Number	29712
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max)
181211blaZ
12/11/2018
Page 4 of 9
Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
Subset Name All Samples
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	blaZ plJB	Standard	13.10	9.89E3	1.46E4	
0	A2	blaZ plJB	Standard	12.32	1.76E4	1.46E4	
0	A3	blaZ plJB	Standard	12.10	2.08E4	1.46E4	
0	A4	Sample 4	Standard	15.94	1.22E3	1.46E3	
0	A5	Sample 4	Standard	15.67	1.48E3	1.46E3	
0	A6	Sample 4	Standard	15.05	2.34E3	1.46E3	
0	A7	Sample 7	Standard	19.59	8.15E1	1.46E2	
0	A8	Sample 7	Standard	18.25	2.20E2	1.46E2	
0	A9	Sample 7	Standard	19.35	9.73E1	1.46E2	
0	A10	Sample 10	Standard	21.72	1.69E1	1.46E1	
0	A11	Sample 10	Standard	21.84	1.55E1	1.46E1	
0	A12	Sample 10	Standard	23.78	3.69E0	1.46E1	
0	B1	Sample 13	Standard	24.44	2.26E0	1.46E0	
0	B2	Sample 13	Standard	24.91	1.60E0	1.46E0	
0	B3	Sample 13	Standard	24.88	1.63E0	1.46E0	
0	B4	Sample 16	Standard	28.10	1.51E-1	1.46E-1	
0	B5	Sample 16	Standard	27.81	1.87E-1	1.46E-1	
0	B6	Sample 16	Standard	28.48	1.13E-1	1.46E-1	
0	B7	Sample 19	Unknown				
0	B8	Sample 20	Unknown				
0	B9	Sample 21	Unknown				
0	B10	Sample 22	Unknown				
0	B11	Sample 23	Unknown				
0	B12	Sample 24	Unknown				
0	C1	H20	Negative Control				
0	C2	H20	Negative Control				
0	C3	H20	Negative Control	35.00	9.16E-4		>
0	C4	T1	Unknown	10.96	4.80E4		E
0	C5	T1	Unknown	11.32	3.70E4		E
0	C6	T1	Unknown	11.09	4.37E4		E
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
181211blaZ
12/11/2018
Page 5 of 9
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	C7	T2	Unknown	15.64	1.51E3		
0	C8	T2	Unknown	11.94	2.33E4		E
0	C9	T2	Unknown	14.99	2.45E3		
0	C10	T3	Unknown	10.62	6.20E4		E
0	C11	T3	Unknown	10.66	6.00E4		E
0	C12	T3	Unknown	10.62	6.19E4		E
0	D1	T4	Unknown				
0	D2	T4	Unknown	33.36	3.07E-3		E
0	D3	T4	Unknown	33.19	3.50E-3		?, E
0	D4	T5	Unknown	35.00	9.16E-4		>, E
0	D5	T5	Unknown	35.00	9.16E-4		>, E
0	D6	T5	Unknown	35.00	9.16E-4		>, E
0	D7	T6	Unknown	32.73	4.89E-3		E
0	D8	T6	Unknown	31.66	1.08E-2		E
0	D9	T6	Unknown	32.28	6.87E-3		E
0	D10	T7	Unknown	35.00	9.16E-4		?, >, E
0	D11	T7	Unknown	35.00	9.16E-4		>, E
0	D12	T7	Unknown	35.00	9.16E-4		>, E
0	E1	Sample 49	Unknown				
0	E2	Sample 50	Unknown				
0	E3	Sample 51	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
181211blaZ
12/11/2018
Page 6 of 9
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown				
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
A1, A2, A3	12.50	0.53	1.61E4	5.62E3
A4, A5, A6	15.55	0.46	1.68E3	5.89E2
A7, A8, A9	19.06	0.72	1.33E2	7.57E1
A10, A11, A12	22.44	1.16	1.20E1	7.24E0
B1, B2, B3	24.74	0.26	1.83E0	3.74E-1
181211blaZ
12/11/2018
Page 7 of 9
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
B4, B5, B6	28.13	0.34	1.50E-1	3.66E-2
C1, C2, C3	35.00		9.16E-4	
C4, C5, C6	11.12	0.18	4.29E4	5.54E3
C7, C8, C9	14.19	1.97	9.09E3	1.23E4
C10, C11, C12	10.63	0.03	6.13E4	1.15E3
D1, D2, D3	33.28	0.12	3.29E-3	3.00E-4
D4, D5, D6	35.00		9.16E-4	1.33E-19
D7, D8, D9	32.22	0.54	7.54E-3	3.03E-3
D10, D11, D12	35.00		9.16E-4	1.33E-19
Amplification Curves
28.257-
5~ 10 15 20 25 30 35 40
Cycles
181211blaZ
12/11/2018
Page 8 of 9
Standard Curve
— Std. curve « Samples
Error: 0.105 Efficiency: 2.095 Slope:-3.114 Ylntercept: 25.54 Link: 0.000
1=H
—I-1-1-1-1-1-1-1-1-r
-0.5 0 0.5 1 1.5 2 2.5 3 3.5 4
Log Concentration
Analysis Notes
181211blaZ
12/11/2018
Page 9 of 9