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LightCycler® 480 Software
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20181030tetK
Experiment
Creation Date	10/31/2018 12:45:49 PM	Last Modified Date	10/31/2018 3:31:17 PM
Operator	LMDM	Owner	LMDM
Start Time	10/31/2018 1:26:40 PM	End Time	10/31/2018 2:30:50 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	02122014blaZkvantifikace Run Protocol	Plate ID	04573900
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		stage 1							
Cycles			Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name Cycles		stage 2							
		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		stage 3							
Cycles		40	Analysis Mode | Quantification						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	Single		00:00:30	2.20		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
	95	Continuous			0.11	5	0	0	0
20181030tetK
10/31/2018
Page 1 of 8
Program Name		cooling							
Cycles		1	Analysis Mode		None				
	Target	Acquisition		Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode		(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
	40	None		00:01:00	2.20		0	0	0
Samples
Sample Count
Subset
96
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	chrJB		A1	
A2	chrJB		A1	
A3	chrJB		A1	
A4	chrJB		A4	
A5	chrJB		A4	
A6	chrJB		A4	
A7	chrJB		A7	
A8	chrJB		A7	
A9	chrJB		A7	
A10	Sample 10		A10	
A11	Sample 10		A10	
A12	Sample 10		A10	
B1	Sample 13		B1	
B2	Sample 13		B1	
B3	Sample 13		B1	
B4	Sample 16		B4	
B5	Sample 16		B4	
B6	Sample 16		B4	
B7	Sample 19		B7	
B8	Sample 19		B7	
B9	Sample 19		B7	
B10	Sample 22		B10	
B11	Sample 22		B10	
B12	Sample 22		B10	
C1	Sample 25		C1	
C2	Sample 25		C1	
C3	Sample 25		C1	
C4	Sample 28		C4	
C5	Sample 28		C4	
C6	Sample 28		C4	
C7	Sample 31		C7	
C8	Sample 31		C7	
C9	Sample 31		C7	
20181030tetK
10/31/2018
Page 2 of 8
Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C10	Sample 36		C12	
C11	Sample 36		C12	
C12	Sample 36		C12	
D1	Tet1 HL		D1	
D2	Tet1 HL		D1	
D3	Tet1 HL		D1	
D4	Tet2 HL		D4	
D5	Tet2 HL		D4	
D6	Tet2 HL		D4	
D7	Tet3 HL		D7	
D8	Tet3 HL		D7	
D9	Tet3 HL		D7	
D10	Cd2hL		D10	
D11	Cd2hL		D10	
D12	Cd2hL		D10	
E1	1		E1	
E2	1		E1	
E3	1		E1	
E4	3		E4	
E5	3		E4	
E6	3		E4	
E7	H20		E7	
E8	H20		E7	
E9	H20		E7	
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
20181030tetK
10/31/2018
Page 3 of 8
Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
Subset Name
All Samples
20181030tetK
10/31/2018
Page 4 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	chrJB	Standard	7.81	4.72E5	3.84E5	
0	A2	chrJB	Standard	8.99	2.49E5	3.84E5	
0	A3	chrJB	Standard	7.78	4.79E5	3.84E5	
0	A4	chrJB	Standard	14.68	1.15E4	3.84E4	
0	A5	chrJB	Standard	12.03	4.80E4	3.84E4	
0	A6	chrJB	Standard	13.14	2.65E4	3.84E4	
0	A7	chrJB	Standard	15.93	5.85E3	3.84E3	
0	A8	chrJB	Standard	18.02	1.89E3	3.84E3	
0	A9	chrJB	Standard	16.16	5.15E3	3.84E3	
0	A10	Sample 10	Standard	21.94	2.28E2	3.84E2	
0	A11	Sample 10	Standard	19.92	6.77E2	3.84E2	
0	A12	Sample 10	Standard	21.75	2.52E2	3.84E2	
0	B1	Sample 13	Standard	22.46	1.72E2	3.84E1	
0	B2	Sample 13	Standard	24.47	5.78E1	3.84E1	
0	B3	Sample 13	Standard	25.83	2.78E1	3.84E1	
0	B4	Sample 16	Standard	30.91	1.78E0	3.84E0	
0	B5	Sample 16	Standard	28.50	6.55E0	3.84E0	
□	B6	Sample 16	Standard			3.84E0	
□	B7	Sample 19	Standard			3.84E-1	
□	B8	Sample 19	Standard			3.84E-1	
□	B9	Sample 19	Standard			3.84E-1	
□	B10	Sample 22	Standard			6.00E3	
□	B11	Sample 22	Standard			6.00E3	
□	B12	Sample 22	Standard			6.00E3	
□	C1	Sample 25	Standard			6.00E2	
□	C2	Sample 25	Standard			6.00E2	
□	C3	Sample 25	Standard			6.00E2	
□	C4	Sample 28	Standard			6.00E1	
□	C5	Sample 28	Standard			6.00E1	
□	C6	Sample 28	Standard			6.00E1	
□	C7	Sample 31	Standard			6.00E0	
□	C8	Sample 31	Standard			6.00E0	
□	C9	Sample 31	Standard			6.00E0	
□	C10	Sample 36	Standard			6.00E-1	
□	C11	Sample 36	Standard			6.00E-1	
□	C12	Sample 36	Standard			6.00E-1	
0	D1	Tet1 HL	Unknown				
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty
20181030tetK
10/31/2018
Page 5 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	D2	Tet1 HL	Unknown				
0	D3	Tet1 HL	Unknown				
0	D4	Tet2 HL	Unknown				
0	D5	Tet2 HL	Unknown				
0	D6	Tet2 HL	Unknown	16.53	4.24E3		
0	D7	Tet3 HL	Unknown	17.72	2.22E3		
0	D8	Tet3 HL	Unknown	17.20	2.94E3		
0	D9	Tet3 HL	Unknown	18.42	1.52E3		
0	D10	Cd2hL	Unknown	32.13	9.20E-1		E
0	D11	Cd2hL	Unknown	35.00	1.95E-1		>, E
0	D12	Cd2hL	Unknown	34.33	2.80E-1		E
0	E1	1	Unknown	6.77	8.25E5		E
0	E2	1	Unknown	6.61	9.03E5		E
0	E3	1	Unknown	7.59	5.30E5		E
0	E4	3	Unknown	30.60	2.10E0		E
0	E5	3	Unknown	30.34	2.43E0		E
0	E6	3	Unknown	30.74	1.96E0		E
0	E7	H20	Negative Control	32.79	6.43E-1		
0	E8	H20	Negative Control	34.07	3.23E-1		
0	E9	H20	Negative Control	32.44	7.77E-1		
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown				
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty
20181030tetK
10/31/2018
Page 6 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
A1, A2, A3	8.19	0.69	4.00E5	1.31E5
A4, A5, A6	13.28	1.33	2.87E4	1.84E4
A7, A8, A9	16.71	1.15	4.30E3	2.12E3
A10, A11, A12	21.20	1.11	3.86E2	2.53E2
B1, B2, B3	24.25	1.70	8.57E1	7.59E1
B4, B5	29.71	1.70	4.16E0	3.37E0
D1, D2, D3				
D4, D5, D6	16.53		4.24E3	
D7, D8, D9	17.78	0.61	2.23E3	7.11E2
D10, D11, D12	33.82	1.50	4.65E-1	3.96E-1
E1, E2, E3	6.99	0.53	7.53E5	1.97E5
E4, E5, E6	30.56	0.20	2.16E0	2.41 E-1
20181030tetK
10/31/2018
Page 7 of 8
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
E7, E8, E9	33.10	0.86	5.81 E-1	2.33E-1
28.609; 26.109^ 23.609^ 21.109^ f 18.G09:
1 16.109^
2 13.609: a;
I 11.109: I 8.609; 6.109^ 3.609^ 1.109:
Amplification Curves
10
15
20
Cycles
25
30
35
40
Standard Curve
Error: 0.244	
Efficiency: 1.717	3CH
Slope: -4.260	2£
Ylntercept: 31.98	2£
Link: 0.000	
	'5 221.
	Q.
	=> 20-:
	1 Tai
	
	14H
	^21.
	1 CK
	8H
Std. curve Samples
3 4 Log Concentration
Analysis Notes
20181030tetK
10/31/2018
Page 8 of 8