C K	C wnt	C rsp	C wnt+rsp	D K	D wnt	D rsp	D wnt+rsp
FF	317	324	887	14375	438	1002	1115	30711
R	7720	28621	43656	53949	89147	96750	79124	106546
FF/R	0.041062176	0.011320359	0.02031794	0.266455356	0.004913233	0.010356589	0.014091805	0.288241698
na K	1.00	0.28	0.49	6.49	1.00	2.11	2.87	58.67
##### Sheet/List 2 #####
Tomas TopFlash		
		
11/5/2019 10:20		
		
Assay was completed successfully.		
		
		
Results		
		
Well	Sample	Luminescence (CPS)
A1	A1	7720
A2	A2	28621
A3	A3	43656
A4	A4	53949
A5	A5	89147
A6	A6	96750
A7	A7	79124
A8	A8	106546
##### Sheet/List 3 #####
Tomas TopFlash				
				
11/5/2019 10:20				
				
Assay was completed successfully.				
				
				
Replicate statistics				
				
Luminescence				
Luminescence (CPS)				
				
Sample	Dilution	N	CV	Mean
A1	All	1	0.00%	7720
A1	1:1	1	0.00%	7720
A2	All	1	0.00%	28621
A2	1:1	1	0.00%	28621
A3	All	1	0.00%	43656
A3	1:1	1	0.00%	43656
A4	All	1	0.00%	53949
A4	1:1	1	0.00%	53949
A5	All	1	0.00%	89147
A5	1:1	1	0.00%	89147
A6	All	1	0.00%	96750
A6	1:1	1	0.00%	96750
A7	All	1	0.00%	79124
A7	1:1	1	0.00%	79124
A8	All	1	0.00%	106546
A8	1:1	1	0.00%	106546
##### Sheet/List 4 #####
Tomas TopFlash	
	
11/5/2019 10:20	
	
Assay was completed successfully.	
	
	
Info	
	
Instrument	
Instrument serial number	3180172
Instrument firmware version	103
	
Software	
Version	0.5.54.0
	
Plate	
Wells	96 (8x12)
Manufacturer	General
Name	
A1 offset (µm)	"14380,11240"
Well distance (µm)	9000
Well diameter (µm)	6500
	
Operations	
	
Luminescence	
Technology	Luminescence
Filter	IR Cutoff
Counting time (s)	1
Aperture	Default
Read mode	Top
Focus (mm)	Default
PMT voltage	Default
Discriminator voltage	Default