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LightCycIer® 480 Software
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20191210TET Katka Experiment
Creation Date	12/10/2019 2:58:33 PM	Last Modified Date	12/12/2019 9:27:38 AM
Operator	LMDM	Owner	LMDM
Start Time	12/10/2019 3:11:25 PM	End Time	12/10/2019 4:27:04 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	02122014blaZkvantifikace Run Protocol, 17032015relativni kvantifikace SGDSGBmecA Run Protocol, 20191204_PrimerOpitmization SYBR Green	Plate ID	07409375
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		Pre-incubation						
Cycles		1              Analysis Mode		None				
	Target	Acquisition	Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode	(hh:mm:ss)	(°C/s)	(per'C)	(°C)	(°C)	(cycles)
	95	None	00:10:00	4.40		0	0	0
Program Name		Amplification						
Cycles		40             Analysis Mode		Quantification				
	Target	Acquisition	Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode	(hh:mm:ss)	(°C/s)	(per'C)	(°C)	(°C)	(cycles)
	95	None	00:00:10	4.40		0	0	0
	58	Single	00:00:20	2.20		0	0	0
	72	None	00:00:20	4.40		0	0	0
Program Name		Melting Curves						
Cycles		1              Analysis Mode		Melting Curves				
	Target	Acquisition	Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode	(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
	95	None	00:00:05	4.40		0	0	0
	65	None	00:01:00	2.20		0	0	0
	97	Continuous		0.11	5	0	0	0
20191210TET Katka
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Page 1 of 9
Program Name		Cooling							
Cycles		1	Analysis Mode		None				
	Target	Acquisition		Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	CC)	Mode		(hh:mm:ss)	(°C/s)	(per'C)	CC)	CC)	(cycles)
	40	None		00:00:10	1.50		0	0	0
Samples
Sample Count
Subset
96
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	10		A1	
A2	1		A2	
A3	0.1		A3	
A4	0.01		A4	
A5	0.001		A5	
A6	Sample 6			
A7	Sample 7			
A8	Sample 8			
A9	Sample 9			
A10	Sample 10			
A11	Sample 11			
A12	h2o		A12	
B1	10		A1	
B2	1		A2	
B3	0.1		A3	
B4	0.01		A4	
B5	0.001		A5	
B6	Sample 18			
B7	Sample 19			
B8	Sample 20			
B9	Sample 21			
B10	Sample 22			
B11	Sample 23			
B12	h2o		A12	
C1	10		A1	
C2	1		A2	
C3	0.1		A3	
C4	0.01		A4	
C5	0.001		A5	
C6	Sample 30			
C7	Sample 31			
C8	Sample 32			
C9	Sample 33			
20191210TET Katka
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Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C10	Sample 34			
C11	Sample 35			
C12	h2o		A12	
D1	PL 3 KAD		D1	
D2	PL 3 KAD		D1	
D3	PL 3 KAD		D1	
D4	Sample 40			
D5	Sample 41			
D6	Sample 42			
D7	Sample 43			
D8	Sample 44			
D9	Sample 45			
D10	Sample 46			
D11	Sample 47			
D12	Sample 48			
E1	PL 7 TET		E1	
E2	PL 7 TET		E1	
E3	PL 7 TET		E1	
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	PL 10 TET		F1	
F2	PL 10 TET		F1	
F3	PL 10 TET		F1	
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
2019121OTET Katka
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Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
G1	JB oba		G1	
G2	JB oba		G1	
G3	JB oba		G1	
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	pT181 COL		H1	
H2	pT181 COL		H1	
H3	pT181 COL		H1	
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Instrument
Name	29712	Serial Number	29712
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	Amplification	Units	
Mode	High Confidence		
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Subset Name All Samples
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	10	Standard	7.58	9.40E3	1.00E4	
0	A2	1	Standard	11.14	8.47E2	1.00E3	
0	A3	0.1	Standard	13.77	1.43E2	1.00E2	
0	A4	0.01	Standard	18.15	7.37E0	1.00E1	
0	A5	0.001	Standard	21.65	1.00E0	1.00E0	
0	A6	Sample 6	Unknown				
0	A7	Sample 7	Unknown				
0	A8	Sample 8	Unknown				
0	A9	Sample 9	Unknown				
0	A10	Sample 10	Unknown				
0	A11	Sample 11	Unknown				
0	A12	h2o	Negative Control	31.16	1.24E-1		
0	B1	10	Standard	7.61	9.23E3	1.00E4	
0	B2	1	Standard	11.09	8.77E2	1.00E3	
0	B3	0.1	Standard	13.61	1.59E2	1.00E2	
0	B4	0.01	Standard	17.95	8.46E0	1.00E1	
0	B5	0.001	Standard	21.52	1.06E0	1.00E0	
0	B6	Sample 18	Unknown				
0	B7	Sample 19	Unknown				
0	B8	Sample 20	Unknown				
0	B9	Sample 21	Unknown				
0	B10	Sample 22	Unknown				
0	B11	Sample 23	Unknown				
0	B12	h2o	Negative Control				
0	C1	10	Standard	7.54	9.64E3	1.00E4	
0	C2	1	Standard	11.11	8.61 E2	1.00E3	
0	C3	0.1	Standard	13.72	1.48E2	1.00E2	
0	C4	0.01	Standard	17.97	8.35E0	1.00E1	
0	C5	0.001	Standard	22.17	7.86E-1	1.00E0	
0	C6	Sample 30	Unknown				
0	C7	Sample 31	Unknown				
0	C8	Sample 32	Unknown				
0	C9	Sample 33	Unknown				
0	C10	Sample 34	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, < - Early Cp call (first five cycles) has higher uncertainty
20191210TET Katka
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Page 5 of 9
Results
lne	Pos	Name	Type	CP	Concentration	Standard	Status
0	C11	Sample 35	Unknown				
0	C12	h2o	Negative Control	35.00	2.15E-1		>
0	D1	PL 3 KAD	Unknown	27.06	1.67E-1		E
0	D2	PL 3 KAD	Unknown	26.72	1.79E-1		E
0	D3	PL 3 KAD	Unknown	27.01	1.69E-1		E
0	D4	Sample 40	Unknown				
0	D5	Sample 41	Unknown				
0	D6	Sample 42	Unknown				
0	D7	Sample 43	Unknown				
0	D8	Sample 44	Unknown				
0	D9	Sample 45	Unknown				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	PL 7 TET	Unknown				
0	E2	PL 7 TET	Unknown	5.00	5.39E4		<, E
0	E3	PL 7 TET	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown				
0	F1	PL 10 TET	Unknown				
0	F2	PL 10 TET	Unknown	5.00	5.39E4		<, E
0	F3	PL 10 TET	Unknown	5.00	5.39E4		<, E
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, < - Early Cp call (first five cycles) has higher uncertainty
20191210TET Katka
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	JB oba	Unknown	7.80	8.09E3		
0	G2	JB oba	Unknown	7.68	8.82E3		
0	G3	JB oba	Unknown	7.71	8.59E3		
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	pT181 COL	Unknown				
0	H2	pT181 COL	Unknown				
0	H3	pT181 COL	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, < - Early Cp call (first five cycles) has higher uncertainty
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
A1, B1, C1	7.58	0.03	9.42E3	2.05E2
A2, B2, C2	11.11	0.03	8.62E2	1.50E1
A3, B3, C3	13.70	0.08	1.50E2	8.25E0
A4, B4, C4	18.02	0.11	8.06E0	6.00E-1
A5, B5, C5	21.78	0.34	9.49E-1	1.45E-1
A12, B12, C12	33.08	2.72	1.70E-1	6.42E-2
D1, D2, D3	26.93	0.19	1.72E-1	6.22E-3
E1, E2, E3	5.00		5.39E4	
20191210TETKatka 12/12/2019 Page 7 of 9
Statistics
Samples	Mean Cp	Std Cp	Mean cone	Std cone
F1, F2, F3	5.00		5.39E4	
G1, G2, G3	7.73	0.06	8.50E3	3.69E2
H1, H2, H3				
26.59:*
24.093^
21.593^
19.093^
I 16.593^
~ 14. ■ť
u
I 11.593:
I
I 9.093: 6.593^ 4.093^ 1.593^ -0.907:
Amplification Curves
10
15
20
Cycles
25
30
35
40
Standard Curve
Error: 0.0400 Efficiency: 1.967 Slope: -3.404 Ylntercept: 21.11 Link: 8.345
Std. curve Samples
Log Concentration
20191210TET Katka
12/12/2019
Page 8 of 9
Analysis Notes
20191210TETKatka 12/12/2019 Page 9 of 9