Popis s v-treat v-untreat Kinetika enzymatické reakce: rovnice Michaelis-Mentenové 20 76 67 20 47 51 60 97 84 60 107 86 v reaction velocity 110 123 98 Vmax maximal velocity 110 139 115 s substrate concentration 220 159 131 Km Michaelis' constant 220 152 124 560 191 144 560 201 158 1100 207 160 1100 200 ##### Sheet/List 2 ##### s v-treat v-untreat s s/v (treat) s/v (untreat) 20 76 67 20 0.263157895 0.298507463 20 47 51 20 0.425531915 0.392156863 60 97 84 60 0.618556701 0.714285714 60 107 86 60 0.560747664 0.697674419 110 123 98 110 0.894308943 1.12244898 110 139 115 110 0.791366906 0.956521739 220 159 131 220 1.383647799 1.679389313 220 152 124 220 1.447368421 1.774193548 560 191 144 560 2.931937173 3.888888889 560 201 158 560 2.786069652 3.544303797 1100 207 160 1100 5.314009662 6.875 1100 200 1100 5.5 ##### Sheet/List 3 ##### s v-treat v-untreat 1/s 1/v (treat) 1/v (untreat) 20 76 67 0.05 0.013157895 0.014925373 20 47 51 0.05 0.021276596 0.019607843 60 97 84 0.016666667 0.010309278 0.011904762 60 107 86 0.016666667 0.009345794 0.011627907 110 123 98 0.009090909 0.008130081 0.010204082 110 139 115 0.009090909 0.007194245 0.008695652 220 159 131 0.004545455 0.006289308 0.007633588 220 152 124 0.004545455 0.006578947 0.008064516 560 191 144 0.001785714 0.005235602 0.006944444 560 201 158 0.001785714 0.004975124 0.006329114 1100 207 160 0.000909091 0.004830918 0.00625 1100 200 0.000909091 0.005 ##### Sheet/List 4 ##### inhibitor konc. substrátu (s) rychlost (v) s/v ano 20 76 0.263157895 ano 20 47 0.425531915 ano 60 97 0.618556701 ano 60 107 0.560747664 ano 110 123 0.894308943 ano 110 139 0.791366906 ano 220 159 1.383647799 ano 220 152 1.447368421 ano 560 191 2.931937173 ano 560 201 2.786069652 ano 1100 207 5.314009662 ano 1100 200 5.5 ne 20 67 0.298507463 ne 20 51 0.392156863 ne 60 84 0.714285714 ne 60 86 0.697674419 ne 110 98 1.12244898 ne 110 115 0.956521739 ne 220 131 1.679389313 ne 220 124 1.774193548 ne 560 144 3.888888889 ne 560 158 3.544303797 ne 1100 160 6.875