Product Data Sheet
Catalog # / Size: 2155630 / 100 tests
Clone: ML5
Isotype: Mouse IgG2a, κ
Reactivity: Human
Preparation: The antibody was purified by affinity
chromatography and conjugated with
Brilliant Violet 510™ under optimal
conditions. The solution is free of
unconjugated Brilliant Violet 510™ and
unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2,
containing 0.09% sodium azide and BSA
(origin USA).
Workshop
Number:
V CD24.5
Concentration: Lot-specific
Human peripheral blood
lymphocytes stained with CD19 APC
and CD24 (clone ML5) Brilliant
Violet 510™ (top) or mouse IgG2a,
κ Brilliant Violet 510™ isotype
control (bottom).
Applications:
Applications: Flow Cytometry
Recommended
Usage:
Each lot of this antibody is quality
control tested by immunofluorescent
staining with flow cytometric analysis.
For flow cytometric staining, the
suggested use of this reagent is ≤5
microL per million cells or 5 microL per
100 microL of whole blood. It is
recommended that the reagent be
titrated for optimal performance for
each application.
Brilliant Violet 510™ excites at 405 nm
and emits at 510 nm. The bandpass
filter 510/50 nm is recommended for
detection, although filter optimization
may be required depending on other
fluorophores used. Be sure to verify
that your cytometer configuration
and software setup are appropriate
for detecting this channel. Refer to
your instrument manual or
manufacturer for support. Brilliant Violet
510™ is a trademark of Sirigen Group
Ltd.
This product is subject to proprietary
rights of Sirigen Inc. and is made and
sold under license from Sirigen Inc. The
purchase of this product conveys to the
buyer a non-transferable right to use
the purchased product for research
Brilliant Violet 510™ anti-human CD24
For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
Sony Biotechnology Inc. 1730 North First Street, San Jose, CA 95112 www.sonybiotechnology.com
purposes only. This product may not be
resold or incorporated in any manner
into another product for resale. Any use
for therapeutics or diagnostics is strictly
prohibited. This product is covered by
U.S. Patent(s), pending patent
applications and foreign equivalents.
Application
Notes:
Additional reported applications (for the
relevant formats) include:
immunofluorescence microscopy3.
Application
References:
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation
Antigens. Oxford University Press. New York.
2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New
York.
3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Description: CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known
as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the
surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells,
and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation
and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24
has been shown to interact with CD62P (P-selectin).
Antigen
References:
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press.
New York.
For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
Sony Biotechnology Inc. 1730 North First Street, San Jose, CA 95112 www.sonybiotechnology.com