C5855
Hmotnostní spektrometrie
Jan Novotný
2019
Intro
Compare two basic ionization methods of biomolecular
MS.
What is the meaning of term “tandem MS spectrometer”.
Explain the abbreviation MALDI-TOF, ESI-FTICR.
Campbell textbook
Calculate the mass change associated with following
mutation:
1. Gly---Ser
2. Ser---Ser-Pi
3. Lys --- N-acetyllysin
What is the biological function of 2 and 3 postranslational modifications?
Campbell textbook
ESI-MS
Estimate the protonation charge
and molecular weight of pure
protein based attached MS.
FA identification
Assign the two common fatty
acids to presented MS spectra
showing fragmental analysis.
Explain the intensity pattern of
bottom spectrum.