Calibrated and calculated when the system was established Position Reaction Time (s) Loop01 19 Loop03 53 Loop05 87 Loop07 119 Loop09 152 Loop11 183 Loop13 214 ##### Sheet/List 2 ##### pH measured after addition of HCl into working buffer. Concentration of HCl determonide by acid-base titration and using NaOH. HCl uL L pH umol of HCl mmol/L Concentration of HCl 0 0 8.60 0 0 0.09876 mol/L 30 0.00003 8.10 2.9628 0.059220468 80 0.00008 7.60 7.9008 0.157763578 160 0.00016 7.10 15.8016 0.315023923 250 0.00025 6.60 24.69 0.491343284 Polynomial function a b c 0.083690006 -1.519786089 6.879264804 ##### Sheet/List 3 ##### Calibration pH 6.60 Calibration pH 7.60 Calibration pH 8.60 Loop01 Loop03 Loop05 Loop07 Loop09 Loop11 Loop13 Loop01 Loop03 Loop05 Loop07 Loop09 Loop11 Loop13 Loop01 Loop03 Loop05 Loop07 Loop09 Loop11 Loop13 1304.8 2029.75 1930.5 2299.59 2004.28 1881.21 1985.09 890.11 1359.92 1328.15 1433.11 1389.79 1383.47 1496.8 486.71 777.74 781.08 912.94 799.62 773.6 820.81 1315.67 1976.22 1994.88 2262.34 2017.11 1957.67 1988.46 869.02 1384.65 1309.39 1450.51 1385.4 1404.88 1450.58 501.65 777.46 791.46 910.59 805.1 780.65 819.62 1268.45 1985.55 2010.89 2238.43 2022.78 1902.65 1970.28 881.21 1375.78 1325.19 1412.72 1391.55 1405.44 1466.26 501.18 775.33 781.39 894.69 816.4 768.02 820.29 1313.67 1997.14 1945.62 2235.3 2010.56 1871.97 1981.1 875.5 1329.31 1338.4 1419.14 1395.6 1357.92 1432.62 393.68 771.23 794.39 902.25 817.91 773.05 821.05 1314.11 1970.6 2002.39 2217.97 2017.83 1887.5 2009.97 877.23 1364.61 1336.96 1436.39 1406.31 1377.24 1437.95 495.25 785.11 783.23 885.44 805.05 777.74 825.34 1323.06 1997.74 1989.1 2229.59 2046.92 1869.8 2003.6 868.7 1385.95 1312.86 1425.22 1394.1 1380.75 1481.04 391.31 770.99 795.69 877.95 828.66 777.65 819.75 1322.1 1972.08 1984.7 2238.97 2020.28 1869.31 2012.19 849.1 1346.21 1313.2 1422.97 1394.11 1351.84 1477.17 502.01 777.21 778.09 886.29 812.52 775.46 808.94 1329.72 1990.71 1998.94 2259.79 2011.41 1887.22 2050.35 884.92 1328.06 1306.95 1438.45 1390.17 1357.17 1477.34 502.65 782.09 795.23 903.91 816.71 791.11 810.77 1322.09 1972.81 2007.14 2304.7 2024.89 1888.56 2028.51 854.1 1319.34 1326.64 1414.03 1387.7 1352.8 1479.67 389.93 770.9 782.45 894.41 810.83 790.26 820.21 1321.24 2013 1940.84 2297.55 1998.06 1912.62 2043.1 859.55 1336.59 1326.12 1452.15 1363.49 1356.11 1484.95 497.65 766.86 789.12 900.46 812.17 795.51 818.71 1325.29 1973.65 1971.46 2340.2 1987.99 1898.29 2018.26 871.99 1353.83 1298.86 1440.59 1376.16 1342.94 1496.38 498.59 780.55 793.04 898.24 796.42 787.33 818.16 1291.64 2011.1 1939.75 2245.29 1982.69 1927.86 2032.03 872.65 1350.31 1310.75 1433.61 1385.01 1353.35 1479.59 500.55 781.69 799.9 902.95 798.01 779.7 805.14 1326.83 2004.1 2001.94 2246.39 1977.85 1919.22 2016.44 877.26 1258.45 1231.53 1433.08 1372.96 1354.12 1487.09 498.26 752.92 789.29 908.12 791.75 788.15 807.16 1314.71 1994.49 1962.12 2268.49 1992.62 1937.58 1991.89 884.62 1315.31 1306.2 1450.64 1363.19 1364.03 1471.35 396.65 767.02 788.23 896.51 792.51 795.05 810.25 Position Reaction Time (s) 1315.79 2009.17 1949.85 2261.01 1996.35 1951.06 1989.44 869.56 1338.9 1283.92 1421.17 1371.42 1382.72 1453.59 495.94 777.77 790.84 892.35 782.83 773.06 819.14 1306.15 2010.99 1970.21 2256.85 1984.39 1901.1 1998.09 871.66 1360.36 1307.08 1406.69 1400.74 1384.42 1469.56 501.18 773.74 787.74 898.51 794.21 776 824.75 1320.15 2017.66 1994.75 2229.26 2019.06 1914.69 2001.31 859 1354.69 1262.64 1395.01 1385.35 1363.46 1434.31 496.29 779.48 780.79 887.79 771.5 766.7 837.77 1307 2045.47 1988.25 2245.66 2031.39 1867.03 2027.45 869.7 1340.55 1302.97 1430.06 1400.61 1382.04 1445.65 491.8 780.15 785.8 885.6 797.24 763.56 824.95 1321.67 1981.71 2004.65 2227.86 2034.84 1900.5 1978.04 874.59 1314.36 1280.78 1429.45 1406.66 1387.04 1442.64 490.12 775.42 786.86 886.92 808.99 771.42 817.5 1336.47 1996.06 2011.47 2215.39 2053.95 1865.28 2030.83 840.99 1359.51 1280.86 1453.42 1397.33 1369.26 1458.58 502.45 788.74 763.81 874.4 811.09 765.71 818.83 1307.6 1999.39 2021.09 1891.42 2003.78 839.26 1287.85 1264.67 1461.94 1403.78 1370.24 1468.76 497.51 765.7 778.45 890.99 803.6 785.27 807.23 863.8 1280.17 1482.19 1369.59 503.48 496.24 496.04 493.51 Average ##### Sheet/List 4 ##### Fluorescence Intensity (CPS) - Averages Time Cal 6.60 Cal 7.60 Cal 8.60 0.05 0.10 0.16 0.21 0.26 0.31 0.37 0.42 <= Concentration of the substrate in the Aq. droplets 19 482.14 498.60 531.58 523.49 533.98 554.93 557.30 563.66 53 868.51 956.66 1066.28 1122.21 1184.69 1273.12 1319.24 1374.04 87 986.20 1134.94 1284.50 1391.88 1506.03 1610.35 1673.53 1739.73 119 1219.45 1424.53 1638.93 1817.49 1981.12 2090.93 2168.71 2280.38 152 1111.94 1397.54 1580.45 1781.37 1921.61 2011.26 2105.67 2200.49 183 1090.47 1458.84 1657.76 1864.68 1978.21 2062.93 2172.83 2248.23 214 1138.64 1635.42 1843.19 2060.15 2187.99 2293.12 2394.23 2473.24 ##### Sheet/List 5 ##### FLUORESCENCE Time [s] 19 53 87 119 152 183 214 pH Time [s] 19 53 87 119 152 183 214 pH 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 pH 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Substrate conc. in aq. plugs Substrate conc. in aq. plugs Flowrate ratio mM Flowrate ratio mM Rxn 14+01 0.05 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 14+01 0.05 0.22 Rxn 13+02 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 13+02 0.10 Rxn 12+03 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 12+03 0.16 Rxn 11+04 0.21 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 11+04 0.21 Rxn 10+05 0.26 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 10+05 0.26 Rxn 09+06 0.31 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 09+06 0.31 Rxn 08+07 0.37 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 08+07 0.37 Rxn 07+08 0.42 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rxn 07+08 0.42 Calibration curves Polynomial a b c 19 0.00 0.00 0.22 53 87 119 152 183 214