Sequence Order Sample [Product] (mM) [Enzyme] (mg/mL) Parameters Visualized data Procedure 1 Buffer 0 - Reaction time 4 min 2 Cal-1 1.5 - 3 Cal-2 1.2 - 4 Cal-3 0.9 - 5 Cal-4 0.6 - 6 Cal-5 0.3 - 7 Buffer 0 - 8 Enzyme 1 To be calculated 0.12 9 Buffer 0 - 10 Enzyme 2 To be calculated 0.2 11 Buffer 0 - Detected peaks Buffer Cal-1 Cal-2 Cal-3 Cal-4 Cal-5 Buffer Enzyme 1 Buffer Enzyme 2 Buffer 1.6012 0.4512 0.8517 1.1097 1.3497 1.5202 1.6581 1.0675 1.5084 0.7857 1.4512 1.6040 0.4287 0.8151 1.0760 1.2778 1.4962 1.6310 1.0096 1.5539 0.7707 1.5199 1.6063 0.4411 0.7821 1.0599 1.3116 1.4665 1.6435 0.9797 1.5499 0.7426 1.5520 1.6011 0.4493 0.8157 1.0732 1.2890 1.4579 1.6354 0.9770 1.5935 0.7532 1.5669 1.5987 0.4566 0.7959 1.0846 1.2337 1.4831 1.5797 0.9738 1.6050 0.8608 1.5978 1.6203 0.4503 0.7834 1.1003 1.3331 1.4690 1.6503 0.9648 1.6444 0.7062 1.6033 1.6059 0.4472 0.7826 1.1002 1.3396 1.4789 1.5723 0.9662 1.6214 0.7087 1.5885 1.6504 0.4413 0.7839 1.0830 1.3509 1.4785 1.5921 0.9642 1.6222 0.7171 1.6017 1.6096 0.4356 0.7659 1.0869 1.3090 1.4653 1.6599 0.9574 1.6501 0.7142 1.6049 1.6154 0.4303 0.7748 1.0754 1.3268 1.4587 1.6448 0.9364 1.6198 0.7086 1.6223 Average Product (mM) Activity (μmol·s-1·mg-1)