G0-G1 47.8% G2-M 13.7% S 31.5% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.2% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 62.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 94.9% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL Click it Jacek(1).fcs Cell cycle 30357 G0-G1 43.5% G2-M 26.2% S 26.3% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.1% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 62.6% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 90.8% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL click it EK(1).fcs Cell cycle 37848 G0-G1 48.0% G2-M 22.6% S 25.2% Cell cycle 99.5% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.4% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 67.4% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 91.1% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL click it JK(1).fcs Cell cycle 46923 G0-G1 50.5% G2-M 19.2% S 22.9% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.9% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 59.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 83.9% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL clickit MJ(1).fcs Cell cycle 33458 G0-G1 39.7% G2-M 22.9% S 0.70% Cell cycle 99.6% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 97.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 77.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 88.2% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso EK.fcs Cell cycle 2429 G0-G1 29.6% G2-M 25.8% S 4.56% Cell cycle 99.5% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.4% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 79.4% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 92.2% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso JK.fcs Cell cycle 6693 G0-G1 38.0% G2-M 23.3% S 0.023% Cell cycle 99.6% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 62.6% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 92.4% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso Jacek.fcs Cell cycle 13310 G0-G1 37.5% G2-M 29.3% S 1.60% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.2% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 55.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 87.9% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso MJ.fcs Cell cycle 11502 G0-G1 44.7% G2-M 19.4% S 21.8% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.3% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 66.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 87.2% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN Click it Jacek(2).fcs Cell cycle 30547 G0-G1 34.8% G2-M 25.5% S 35.5% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 67.2% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 89.3% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it EK(2).fcs Cell cycle 32486 G0-G1 40.9% G2-M 28.3% S 26.7% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.0% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 77.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 90.4% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it JK(2).fcs Cell cycle 32074 G0-G1 54.7% G2-M 32.8% S 2.00% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 64.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 89.5% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it MJ(1).fcs Cell cycle 72238 G0-G1 15.2% G2-M 38.9% S 2.04% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 73.6% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 92.6% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso EK(1).fcs Cell cycle 6071 G0-G1 31.1% G2-M 29.0% S 2.39% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.1% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 85.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 95.0% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso JK(1).fcs Cell cycle 10471 G0-G1 39.0% G2-M 33.9% S 0.57% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.3% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 63.9% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 92.5% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso Jacek(1).fcs Cell cycle 12724 G0-G1 41.7% G2-M 27.9% S 0.26% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.3% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 63.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 94.3% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 clickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso MJ.fcs Cell cycle 31091