G0-G1 40.4% G2-M 19.7% S 28.1% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 97.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 64.6% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 97.6% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL Click it KC(1).fcs Cell cycle 18063 G0-G1 52.0% G2-M 24.6% S 6.74% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.3% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 54.6% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 98.7% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL click it AB(1).fcs Cell cycle 27908 G0-G1 39.9% G2-M 20.3% S 29.4% Cell cycle 99.2% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 97.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 71.4% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 93.9% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL click it BH(1).fcs Cell cycle 19485 G0-G1 43.0% G2-M 21.2% S 0.38% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 62.0% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 94.5% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso AB.fcs Cell cycle 2652 G0-G1 37.6% G2-M 27.4% S 2.55% Cell cycle 99.5% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.3% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 76.9% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 92.6% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso BH.fcs Cell cycle 3916 G0-G1 38.3% G2-M 29.4% S 0.10% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 70.7% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 97.4% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso JB.fcs Cell cycle 12764 G0-G1 31.4% G2-M 37.2% S 0% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 96.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 72.9% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 83.8% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso KC.fcs Cell cycle 807 G0-G1 40.7% G2-M 26.8% S 5.51E-3% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 70.8% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 97.6% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRL iso MB.fcs Cell cycle 18163 G0-G1 41.1% G2-M 17.1% S 23.1% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.2% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 71.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 99.0% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRLclick it JB(1).fcs Cell cycle 20952 G0-G1 41.7% G2-M 17.8% S 25.6% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.1% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 70.9% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 98.6% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 CTRLclick it MB(1).fcs Cell cycle 30046 G0-G1 40.2% G2-M 37.2% S 0.14% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 56.4% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 99.2% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it AB(1).fcs Cell cycle 28840 G0-G1 40.5% G2-M 23.2% S 25.1% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.0% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 78.0% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 98.3% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it BH(2).fcs Cell cycle 22466 G0-G1 50.2% G2-M 18.4% S 16.4% Cell cycle 99.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.5% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 79.8% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 99.1% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it JB(2).fcs Cell cycle 23529 G0-G1 53.3% G2-M 16.6% S 12.6% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.2% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 79.4% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 99.1% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click it MB(2).fcs Cell cycle 29914 G0-G1 48.8% G2-M 23.7% S 23.5% Cell cycle 99.8% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.0% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 64.7% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 99.3% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN click itKC(4)1.fcs Cell cycle 28492 G0-G1 41.2% G2-M 29.8% S 0.038% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 64.5% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 98.0% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso AB.fcs Cell cycle 23509 G0-G1 38.2% G2-M 26.1% S 10.6% Cell cycle 97.1% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 84.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 80.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 61.9% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso BH(1).fcs Cell cycle 199 G0-G1 44.2% G2-M 25.7% S 0.13% Cell cycle 93.7% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.7% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 81.3% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 97.8% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso JB(1).fcs Cell cycle 8722 G0-G1 42.6% G2-M 27.6% S 0.33% Cell cycle 99.9% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 99.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 80.7% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 97.4% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN iso MB(1).fcs Cell cycle 27825 G0-G1 23.3% G2-M 43.1% S 0.39% Cell cycle 99.3% VL1-A :: VL1 PO-PRO-A x VL1-W :: VL1 PO-PRO-W Compact population 98.6% FSC-A :: FSC-A x SSC-A :: SSC-A Single Cells 65.1% FSC-A :: FSC-A x FSC-H :: FSC-H Live cells 91.0% BL3-A :: BL3 LD Red-A x SSC-A :: SSC-A 0 1.0M 2.0M 3.0M 4.0M 5.0M PO-PRO (B. cyklus) 10 1 10 2 10 3 10 4 10 5 10 6 10 7 ClickiTEduA488(Proliferace) PO-PRO test DU145 DU145 MLN isoKC(2).fcs Cell cycle 1528