L# Date Time Lecturer Topic Chapter reading
1 7.10. 14:00 - 16:30 Pavel Plevka
Development of X-ray crystallography, crystallization of
macromolecules, phase diagram, Crystal symmetry,
symmetry operators, point groups, space groups.
Drenth: 1, 2, 3
2 14.10. 14:00 - 16:30 Pavel Plevka
Diffraction of light by electrons, atoms, unit cell, crystal.
Bragg's law. Diffraction images and indexing.
Drenth: 4
3 21.10. 14:00 - 16:30 Pavel Plevka
Fourier transform, structure factor, intensity of
diffraction spots.
Drenth: 4, 5
4 28.10. 14:00 - 16:30 Pavel Plevka
Solutions to phase problem in X-ray crystallography.
Isomorphous replacement, SAD, MAD, Molecular
replacement. Rotation and translation function. Model
building and refinement.
Drenth: 7, 10
5 4.11. 14:00 - 16:30 Tibor Füzik
Electron microscope. Interaction of electrons with
matter, electron imaging. Amplitude and phase
contrast. Contrast transfer function.
Frank: 1, 2
6 11.11. 14:00 - 16:30 Tibor Füzik
Fourier transform and its properties, convolution, point
spread function.
Frank: 2
7 18.11. 14:00 - 16:30 Jiří Nováček
Analysis of electron micrographs. 2D classification.
Principal component analysis.
Frank: 3, 4
8 25.11. 14:00 - 16:30 Jiří Nováček
Three dimensional reconstruction - single particle
reconstruction and tomogram calculation. 3D
classification.
Frank: 5
9 2.12. 14:00 - 16:30 Jiří Nováček
Improving cryo-EM reconstruction, particle polishing,
Ewalds, sphere correction, per particle CTF, … Model
building and refinement. Detection of errors, validation
and detection of mistakes.
Frank: 6
10 TBD TBD Holger Stark State-of-the-art cryo-EM of macromolecular complexes.
11 TBD TBD Holger Stark State-of-the-art cryo-EM of macromolecular complexes.