Molecular Biotechnology
Microfluidics & Lab-on-chip, 25/11/2020
Michal Vašina (michal.vasina@recetox.muni.cz)
David Kovář (kovard@mail.muni.cz)

•Protocol
•1 DOC/PDF document (name/date – see template in study materials)
•Homeworks (from Textbook)
•Analyzed data from this exercise (2 microfluidic platforms)
•Deadline: 9/12/2020 23:59 (Homework vaults)
•
1.Enzyme activity platform (Dropix)
1.Introduction & data collection
2.Exercise: Data analysis
•
2.Enzyme kinetics platform (CBMP)
1.Introduction & data collection
2.Exercise: Data analysis
Organization info

1. Enzyme activity platform (Dropix)
•


•Capillary droplet microfluidic platform for enzyme activity characterization
Anal.Chem. 2019, 91, 10008−10015
1 – sampler 6 – laser source
2 – droplet generator 7 – cut-off filter
3 – incubation/substrate chamber 8 – detector
4 – detection cube 9 – temperature controller
5 – syringe pump
Dropix enzyme activity platform

•Capillary droplet microfluidic platform for enzyme activity characterization
Anal.Chem. 2019, 91, 10008−10015
1 – sampler 6 – laser source
2 – droplet generator 7 – cut-off filter
3 – incubation/substrate chamber 8 – detector
4 – detection cube 9 – temperature controller
5 – syringe pump
Dropix microfluidic platform

1 – sampler 6 – laser source
2 – droplet generator 7 – cut-off filter
3 – incubation/substrate chamber 8 – detector
4 – detection cube 9 – temperature controller
5 – syringe pump
•Capillary droplet microfluidic platform for enzyme activity characterization
Anal.Chem. 2019, 91, 10008−10015
Dropix microfluidic platform

•Capillary droplet microfluidic platform for enzyme activity characterization
Anal.Chem. 2019, 91, 10008−10015
1 – sampler 6 – laser source
2 – droplet generator 7 – cut-off filter
3 – incubation/substrate chamber 8 – detector
4 – detection cube 9 – temperature controller
5 – syringe pump
Dropix microfluidic platform

In real



Data collection



1. Exercise
•Sample_dataset_Dropix.xlsx
•
•Protocol requirements
•Graph: calibration curve
•Product concentration for Enzyme 1 & Enzyme 2
•Specific activities of Enzyme 1 & Enzyme 2
•Comparison (short conclusion)
•

2. Enzyme kinetics platform (CBMP)
•


CBMP – Capillary Bioplatform



„Droplet Train“



2. Exercise
•Sample_dataset_CBMP.xlsx
•
•Protocol requirements
•Graphs: calibration curve; progress reaction curves
•Bonus: calculate kinetic parameters of Enzyme 3
•

Homework
•


Droplet merging (homework)



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Inverted microscope / Olympus

15
35
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Oil
Cell matrix
Outlet

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Oil
Cell matrix
Outlet
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Microfluidic chip
cells encapsulation

Spacing oil
Droplets
Spacing oil
Collection
Waste
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70

Spacing oil
Droplets
Spacing oil
Collection
Waste
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30
50
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70
Microfluidic chip
droplets sorting

Batch-based screening process vs. FADS (or FACS)
22/17
570 years
continuously
6 people
15 days
continuously
1 people
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