Effect of integrated pest management (IPM) on abundance and trophic interactions of arthropod
predators inside pear orchards during winter and spring
Domagoj Gajski
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Psyllids - Cacopsylla spp.
§Main pest in pear orchards:
1)Extract nutrients from pear trees
2)Induce development of sooty moulds
3)Transmit harmful phytoplasmas that cause pear decline
§Resistant to pesticides due to their polyvoltine nature
§Active already in early spring  è high abundance in early summer

Naturaly occuring predators
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Specialists – Anthocoris spp.
Generalist predators
Coccinellids
Spiders

Experimental design
Netčice
Morkuvky
Stary Liskovec
Kobyli
Litenčice
Velke Bilovice 1
Velke Bilovice 2
Mikulčice
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Ecological management
- control
Integrated pest management
PEAR ORCHARDS:
Visited in December, February and April!

Experimental design



Experimental design
No description available.
Installing cardboard traps around trunks and branches
No description available.
Sorting samples and identification
December è February è April

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•Over 10 000 arthropod samples, out of which 4139 were predators.
•
•Out of the predators, more than 80 % were spiders.

Effect of pest management type on presence of some spider groups
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Aims during this course:
•Compare abundancies of arthropod clades or predatory guilds between:
a)differently treated orchards (IPM vs EKO)
b)samples taken at the margin and the center of the same orchard
•
a)
•Compare species richness, diversity and size differences of all arthropods between:
a)differently treated orchards (IPM vs EKO)
b)samples taken at the margin and the center of the same orchard
c)
c)
•Assess if there is a relationship between presence of some specific arthropod groups on the same
tree (e.g. if some predator inhabits a tree due to the presence of some specific prey).
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Aims during this course:
•Have data about seasonal abundance changes of predators and psyllids during spring
•
•Would assess their relationship through regression charts and calculating the significance of
their relationship, taking into account the seasonal change and abundance change
•Learn how to make such beautiful charts :)
•
•

How to study the diet of bugs – molecular methods
Spider anatomy - Spidentify
DNA extraction — Science Learning Hub Multiple Sequence Alignment with MUSCLE - Unipro UGENE
Bioinformatics and Identification of sequences
Macro Photos Of Cute And Cuddly Jumping Spiders by Thomas Shahan | Bored Panda

Transformation of information
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Each prey will be turned into 1 (present) or 0 (not present)
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Thank you for your attention!
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