MAN0003748 | MP10419	 Revision A.0
For Research Use Only. Not for use in diagnostic procedures.
Click-iT®
EdU Flow Cytometry Assay Kits
*100 tests*
Catalog numbers C10419, C10420
Table 1 Contents and storage
Material Amount Concentration Storage* Stability
EdU (Component A) 20 mg NA
•	2°C–8°C
•	Desiccate
•	Protect from light
•	Do not freeze
When stored
as directed,
this kit is
stable for up
to 1 year after
receipt.
Alexa Fluor®
488 azide (Cat. no. C10420) or
Alexa Fluor®
647 azide (Cat. no. C10419)
(Component B)
1 vial NA
Dimethylsulfoxide (DMSO) (Component C) 8.5 mL NA
Click-iT®
fixative (Component D) 10 mL
4% paraformaldehyde
in PBS
Click-iT®
saponin-based permeabilization
and wash reagent (Component E)
100 mL 10X solution
CuSO4 (Component F) 1 mL
100 mM aqueous
solution
Click-iT®
EdU buffer additive (Component G) 400 mg NA
*These storage conditions are appropriate when storing the entire kit upon receipt. For optimal storage conditions for each
component, see vial labels. NA = Not applicable.
Number of assays: Sufficient material is supplied for 100 reactions, based on the protocol below.
Approximate fluorescence excitation/emission maxima: Alexa Fluor®
647 azide: 650/670 nm; Alexa Fluor®
488 azide: 495/519 nm.
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 2
Figure 1 Fluorescence signal from Alexa Fluor®
488 and Alexa Fluor®
647 Click-iT®
EdU Flow Cytometry Assay
Kits. Jurkat (human T-cell leukemia) cells were treated with 10 µM EdU for 2 hours and detected according to the
recommended staining protocol. The figures show a clear separation of proliferating cells which have incorporated
EdU and nonproliferating cells which have not. Panel A shows data from cells labeled with Alexa Fluor®
488
azide analyzed on an Attune®
Acoustic Focusing Cytometer using 488 nm excitation and a 530/30 nm bandpass
emission filter; Panel B shows data from cells labeled with Alexa Fluor®
 647 azide analyzed on a flow cytometer
using 633 nm excitation and a 660/20 nm bandpass emission filter.
Introduction
Measuring a cell’s ability to proliferate is a fundamental method for assessing cell
health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate
method is by directly measuring DNA synthesis. Initially, this was performed by
incorporation of radioactive nucleosides, i.e., 3
H-thymidine. This method was replaced
by antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU). The
Click‑iT®
EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU
(5-ethynyl-2´-deoxyuridine) is a nucleoside analog to thymidine and is incorporated
into DNA during active DNA synthesis. Detection is based on a click reaction,1–4
a
copper catalyzed covalent reaction between an azide and an alkyne. In this application,
the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to
Alexa Fluor®
647 dye or Alexa Fluor®
488 dye. Standard flow cytometry methods are
used for determining the percentage of S-phase cells in the population (Figure 1).
The advantage of Click-iT®
EdU labeling is that the small size of the dye azide allows
for efficient detection of the incorporated EdU using mild conditions. Standard
aldehyde-based fixation and detergent permeabilization are sufficient for the Click‑iT®
detection reagent to gain access to the DNA. This is in contrast to BrdU assays that
require DNA denaturation (using acid, heat, or digestion with DNase) to expose the
BrdU so that it may be detected with an anti-BrdU antibody. Sample processing for
the BrdU assay can result in signal alteration of the cell cycle distribution as well as
the destruction of antigen recognition sites when using the acid denaturation method.
In contrast, the EdU cell proliferation kit is compatible with cell cycle dyes (Figure 2,
page 3). The EdU assay can also be multiplexed with antibodies against surface and
intracellular markers. However, some reagents or antibody conjugates may not be
compatible with the Click-iT®
EdU detection reaction and may need some additional
steps to ensure compatibility (see Table 2, page 3 for details).
B
Click-iT®
EdU Alexa Fluor®
647 fluorescence
Count
0
50
100
150
200
102
10 103
104
105
A
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 3
Table 2 Click-iT®
EdU detection reagent compatibility
Fluorescent molecule Compatibility*
Qdot®
nanocrystals
Use Qdot®
nanocrystals after the Click-iT®
detection
reaction.
Fluorescent proteins (GFP)
Use anti-GFP antibodies before the Click-iT®
detection
reaction or use organic dye-based reagents for protein
expression detection.
Organic dyes such as Alexa Fluor®
dyes,
fluorescein (FITC)
Compatible
PerCP, allophycocyanin (APC) and APC-based
tandems (i.e., Alexa Fluor®
680-APC)
Compatible
R-phycoerythrin (R-PE) and R-PE based
tandems (i.e., Alexa Fluor®
610-RPE)
Use R-PE and R-PE-based tandems after the
Click‑iT®
detection reaction.
TC-FlAsH™
/TC-ReAsH™
reagents
Detect the tetracysteine (TC) tag with TC-FlAsH™
or
TC-ReAsH™
reagents before the Click-iT®
detection
reaction.
*Compatibility indicates whether fluorescent molecule itself or the detection methods involve
components that are unstable in the presence of copper catalyst used for the Click-iT®
EdU detection
reaction. Not all anti-GFP antibodies recognize the same antigen site. Rabbit and chicken anti-GFP
antibodies perform well, but the mouse monoclonal antibodies tested do not generate an acceptable
amount of fluorescence and are not recommended for this application.
Figure 2 Dual parameter plot of Click-iT®
EdU Alexa Fluor®
488 and FxCycle™
Violet. Jurkat (human T-cell leukemia)
cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. Data
were collected and analyzed using an Attune®
Acoustic Cytometer using 488 nm excitation and a 530/30 bandpass
for detection of the EdU Alexa Fluor®
488 azide and 405 nm excitation and a 450/40 bandpass for detection of the
FxCycle™
Violet fluorescence. This figure combines DNA content with EdU; cells that are positive for both labels
are in S-phase of the cell cycle.
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 4
Before starting
Materials required but
not provided •	 1% bovine serum albumin (BSA) in phosphate buffered saline (PBS), pH 7.1–pH 7.4
•	 Buffered saline solution, such as PBS, D-PBS, or TBS
•	 Deionized water or 18 MΩ purified water
•	 12 × 75-mm tubes, or other flow cytometry tubes
Cautions •	 DMSO (Component C), provided as a solvent in this kit, is known to facilitate the
entry of organic molecules into tissues. Handle reagents containing DMSO using
equipment and practices appropriate for the hazards posed by such materials.
Dispose of the reagents in compliance with all pertaining local regulations.
•	 Click-iT®
fixative (Component D) contains paraformaldehyde, which is harmful. Use
with appropriate precautions.
•	 Click-iT®
saponin-based permeabilization and wash reagent (Component E) contains
sodium azide, which yields highly toxic hydrazoic acid under acidic conditions.
Dilute azide compounds in running water before discarding to avoid accumulation
of potentially explosive deposits in plumbing.
Preparing reagents
	 1.1	 Allow vials to warm to room temperature before opening.
	 1.2 	To prepare a 10 mM solution of EdU, add 8 mL of DMSO (Component C) or aqueous
solution (PBS) to Component A and mix well. After use, store any remaining stock
solution at ≤–20°C. When stored as directed, the stock solution is stable for up to 1 year.
	 1.3 	To prepare a working solution of Alexa Fluor®
647 azide (Cat. no. C10419) or
Alexa Fluor®
 488 azide (Cat. no. C10420), add 260 µL of DMSO to Component B and
mix well. After use, store any remaining working solution at ≤–20°C. When stored as
directed, this working solution is stable for up to 1 year.
	 1.4 	To prepare 1 mL of 1X Click-iT®
saponin-based permeabilization and wash reagent, add
100 mL of Component E to 900 mL of 1% BSA in PBS. Smaller amounts can be prepared
by diluting a volume of Component E 1:10 with 1% BSA in PBS. After use, store any
remaining solutions at 2°C–8°C. When stored as directed, the 1X solution is stable for
6 months and the 10X solution is stable for 12 months after receipt.
Note: Component E contains sodium azide (see Cautions, page 4).
	 1.5 	To make a 10X stock solution of the Click-iT®
EdU buffer additive (Component G), add
2 mL of deionized water to the vial and mix until the Click-iT®
EdU buffer additive is
fully dissolved. After use, store any remaining stock solution at ≤–20˚C. When stored as
directed, the stock solution is stable for up to 1 year.
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 5
Experimental protocols
The following protocol was developed with Jurkat cells, a human T cell line, and using
an EdU concentration of 10 µM, and can be adapted for any cell type. Growth medium,
cell density, cell type variations, and other factors may influence labeling. In initial
experiments, we recommend testing a range of EdU concentrations to determine the
optimal concentration for your cell type and experimental conditions. If currently using a
BrdU based assay for cell proliferation, a similar concentration to BrdU is a good starting
concentration for EdU. If using whole blood as the sample, we recommend heparin as
the anticoagulant for collection.
Figure 3 Workflow diagram for the Click-iT®
EdU Flow Cytometry Assay Kits.
Incubate sample with Click-iT®
EdU
Harvest cells
(Optional) Treat cells with antibodies to cell surface antigens
Fix and permeabilize cells
(Optional) Treat cells with antibodies to intracellular antigens
Detection of Click-iT®
EdU
(Optional) Treat cells with cell cycle stain
Analyze cells by flow cytometry
Labeling cells with EdU
	 2.1 	Suspend the cells in an appropriate tissue culture medium to obtain optimal conditions
for cell growth. Disturbing the cells by temperature changes or washing prior to
incubation with EdU slows the growth of the cells during incorporation.
	 2.2 	Add EdU to the culture medium at the desired final concentration and mix well. We
recommend a starting concentration of 10 μM for 1–2 hours. For longer incubations, use
lower concentrations. For shorter incubations, higher concentrations may be required.
For a negative staining control, include cells from the same population that have not
been treated with EdU.
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 6
	 2.3 	Incubate under conditions optimal for cell type for the desired length of time. Altering
the amount of time the cells are exposed to EdU or subjecting the cells to pulse labeling
with EdU allows the evaluation of various DNA synthesis and proliferation parameters.
Effective time intervals for pulse labeling and the length of each pulse depend on the
cell growth rate.
	 2.4 	Harvest cells and proceed immediately to step 3.1 if performing antibody surface
labeling; otherwise continue to step 4.1.
Staining cell-surface antigens
with antibodies (optional)
	 3.1 	Wash cells once with 3 mL of 1% BSA in PBS, pellet cells by centrifugation, and remove
supernatant.
	 3.2 	Dislodge the pellet and resuspend cells at 1 × 107
cells/mL in 1% BSA in PBS.
	 3.3 	Add 100 µL of cell suspension or whole blood sample to flow tubes.
	 3.4 	Add surface antibodies and mix well (Table 2, page 3).
Note: Do not use PE, PE-tandem, or Qdot®
antibody conjugates before performing the
click reaction; wait until step 6.1 for labeling with these fluorophores.
	 3.5 	Incubate for the recommended time and temperature, protected from light.
	 3.6 	Proceed to step 4.1 for cell fixation.
Fixation and permeabilization The Click-iT®
saponin-based permeabilization and wash reagent can be used with
whole blood or cell suspensions containing red blood cells, as well as with cell
suspensions containing more than one cell type. This permeabilization and wash
reagent maintains the morphological light scatter characteristics of leukocytes while
lysing red blood cells.
	 4.1 	Wash the cells once with 3 mL of 1% BSA in PBS, pellet the cells, and remove the
supernatant.
	 4.2 	Dislodge the pellet, add 100 µL of Click-iT®
fixative (Component D), and mix well.
	 4.3 	Incubate the cells for 15 minutes at room temperature, protected from light.
	 4.4 	Wash the cells with 3 mL of 1% BSA in PBS, pellet the cells, and remove the supernatant.
Repeat the wash step if red blood cells or hemoglobin are present in the sample.
Remove all residual red blood cell debris and hemoglobin before proceeding.
	 4.5 	Dislodge the cell pellet and resuspend the cells in 100 μL of 1X Click-iT®
saponin-based
permeabilization and wash reagent (prepared in step 1.4), and mix well. Incubate the
cells for 15 minutes or proceed directly to step 5.1 for click labeling.
Click-iT®
reaction
	 5.1 	Prepare 1X Click-iT®
EdU buffer additive by diluting the 10X stock solution (prepared
in step 1.5) 1:10 in deionized water.
	 5.2 	Prepare the Click-iT®
reaction cocktail according to Table 3 (page 7).
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 7
Note: Use the Click-iT®
reaction cocktail within 15 minutes of preparation.
	 5.3	 Add 0.5 mL of Click-iT®
reaction cocktail to each tube and mix well.
	 5.4 	Incubate the reaction mixture for 30 minutes at room temperature, protected from light.
	 5.5 	Wash the cells once with 3 mL of 1X Click-iT®
saponin-based permeabilization and
wash reagent (prepared in step 1.4), pellet the cells, and remove the supernatant.
Dislodge the cell pellet and resuspend the cells in 100 μL of 1X Click-iT®
saponin-based
permeabilization and wash reagent, if proceeding with intracellular antibody labeleing
in step 6.1. Otherwise, add 500 μL of 1X Click-iT®
saponin-based permeabilization and
wash reagent and proceed with step 7.1 for staining the cells for DNA content, or with
step 8.1 for analyzing the cells on a flow cytometer.
Staining intracellular or
surface antigens (optional)
	 6.1	 Add antibodies against intracellular antigens or against surface antigens that use RPE,
PE‑tandem, or Qdot®
antibody conjugates. Mix well.
	 6.2 	Incubate the tubes for the time and temperature required for antibody staining,
protected from light.
	 6.3 	Wash each tube with 3 mL of 1X Click-iT®
saponin-based permeabilization and wash
reagent (prepared in step 1.4), pellet the cells, and remove the supernatant. Dislodge
the cell pellet and resuspend the cells in 500 μL of 1X Click-iT®
saponin-based
permeabilization and wash reagent, and proceed with step 7.1 for staining the cells for
DNA content, or with step 8.1 for analyzing the cells on a flow cytometer.
Table 3 Click-iT®
reaction cocktails
Reaction
components
Number of reactions
1 2 5 10 15 30 50 100
PBS, D-PBS, or TBS 438 µL 875 µL 2.19 mL 4.38 mL 6.57 mL 13.2 mL 21.9 mL 43.8 mL
CuSO4 (Component F) 10 µL 20 µL 50 µL 100 µL 150 µL 300 µL 500 µL 1 mL
Fluorescent dye azide
(prepared in step 1.3)
2.5 µL 5 µL 12.5 µL 25 µL 37.5 µL 75 µL 125 µL 250 µL
Reaction Buffer
Additive (prepared in
step 5.1)
50 µL 100 µL 250 µL 500 µL 750 µL 1.5 mL 2.5 mL 5 mL
Total reaction volume 500 µL 1 mL 2.5 mL 5 mL 7.5 mL 15 mL 25 mL 50 mL
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 8
Staining cells for DNA content
(optional)
	 7.1 	If necessary, add Ribonuclease A to each tube and mix (Table 4).
	 7.2 	Add the appropriate DNA stain to each tube, mix well, and incubate as recommended
for each DNA stain.
Analysis by flow cytometry If measuring total DNA content on a traditional flow cytometer using hydrodynamic
focusing, use a low flow rate during acquisition. If using the Attune®
Acoustic
Focusing Cytometer, all collection rates may be used without loss of signal integrity
if the event rate is kept below 10,000 events per second. However, for each sample
within an experiment, the same collection rate and cell concentration should be used.
The fluorescent signal generated by DNA content stains is best detected with linear
amplification. The fluorescent signal generated by Click-iT®
EdU labeling is best
detected with logarithmic amplification.
	 8.1 	Analyze the cells using a flow cytometer.
•	 For the detection of EdU with Alexa Fluor®
647 azide use 633/635 nm excitation with
a red emission filter (660/20 nm or similar).
•	 For the detection of EdU with Alexa Fluor®
488 azide, use 488 nm excitation with a
green emission filter (530/30 nm or similar).
Table 4 Click-iT®
EdU compatibility with DNA content stains
DNA Content Stain
Click-iT®
EdU Stain Compatibility
RNase required?
Alexa Fluor®
647 Alexa Fluor®
488
FxCycle™
Violet Yes Yes No
Propidium iodide (PI) Yes No* Yes
SYTOX®
AADvanced™
Yes Yes Yes
FxCycle™
Far Red No Yes Yes
* You can use propidium iodide (PI) for cell cycle analysis with Alexa Fluor®
488 dye; however,
this may reduce the Click-iT®
Alexa Fluor®
488 EdU signal. Other DNA stains are recommended
instead.
Note: The complete excitation and emission spectra for the DNA content stains are available at
www.lifetechnologies.com/spectraviewer.
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 9
Dual pulse labeling
using EdU and BrdU
Follow these guidelines to perform dual labeling of cultured cells by combining EdU
with BrdU labeling.
•	 Use EdU for the first pulse and BrdU for the second pulse.
•	 Removal of EdU from the cell culture media is not required when BrdU is added as
the second label.
•	 Addition of BrdU to culture media containing EdU results in preferential
incorporation of BrdU into the DNA with the exclusion of EdU, while simultaneous
addition of EdU with equimolar or half equimolar BrdU to the media results in only
BrdU incorporation. This simplifies the dual labeling protocol by eliminating the
wash step normally required to remove the first label from the culture media prior to
addition of the second label.
•	 Process the cells after dual pulse labeling using a proven BrdU protocol.
•	 After the DNA denaturation step in the BrdU protocol, click label the cells first for
the detection of EdU, and then follow with an antibody labeling protocol for the
detection of BrdU.
•	 Select a BrdU antibody which does not have cross-reactivity to EdU, such as clone
MoBu‑1 (Cat. nos. B35129, B35139, B35140, B35141).
EdU–Alexa Fluor®
647 azide
BrdU–AlexaFluor®
488
101
102
103
104
105
0
102
103
104
105
-45
-58
BrdU–
EdU+
BrdU–
EdU–
BrdU+
EdU+
BrdU+
EdU–
Figure 4 Dual pulse labeling with EdU and BrdU. Jurkat cells were first treated with 20 mM EdU for 1 hour. Without
washing or removal of the EdU, BrdU was added at a 10 μM concentration, and the cells were incubated for 1 hour.
The cells were harvested, washed, fixed with 70% ice-cold ethanol and stored at 4°C for 96 hours, followed by
an HCl-based denaturation procedure. EdU was detected with Alexa Fluor®
488 azide using the Click-iT®
EdU
Flow Cytometry Kit (Cat. no. C10420). BrdU was then detected with anti-BrdU, Alexa Fluor®
647 conjugate (Cat.
no. A21305). SYTOX®
Blue nucleic acid stain (Cat. no. S11348) with RNase was used to detect DNA content. The
labeled cells were analyzed by flow cytometry using 488 nm excitation with a 530/30 nm bandpass to detect EdU,
633 nm excitation with a 660/20 nm bandpass to detect BrdU, and 405 nm excitation with a 450/50 nm bandpass
to detect DNA content. Four populations of cells are distinguished in the EdU vs BrdU plot: cells which are positive
for both (Q2, upper right), cells which are negative for both (Q3, lower left), EdU positive but BrdU negative (Q4,
lower right), and cells which are positive for BrdU but negative for EdU (Q1, upper left).
Click-iT®
EdU Flow Cytometry Assay Kits *100 tests* | 10
References
1. Chembiochem 4, 1147 (2003); 2. J Am Chem Soc 125, 3192 (2003); 3. Angew Chem Int Ed Engl 41, 2596 (2002); 4. Angew Chem Int Ed
Engl 40, 2004 (2001); 5. BioTechniques 44, 927 (2008); 6. Curr Protoc Cytom 55, 7.38.1 (2011).
Product List	Current prices may be obtained from our website or from our Customer Service Department.
Cat no. Product name Unit size
C10419 Click-iT®
EdU Alexa Fluor®
647 Flow Cytometry Assay Kit *100 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10420 Click-iT®
EdU Alexa Fluor®
488 Flow Cytometry Assay Kit *100 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
Related products
C10418 Click-iT®
EdU Pacific Blue™
Flow Cytometry Assay Kit *50 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10424 Click-iT®
EdU Alexa Fluor®
647 Flow Cytometry Assay Kit *50 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10425 Click-iT®
EdU Alexa Fluor®
488 Flow Cytometry Assay Kit *50 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10632 Click-iT®
Plus EdU Alexa Fluor®
488 Flow Cytometry Assay Kit *50 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10633 Click-iT®
Plus EdU Alexa Fluor®
488 Flow Cytometry Assay Kit *100 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10634 Click-iT®
Plus EdU Alexa Fluor®
647 Flow Cytometry Assay Kit *50 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
C10635 Click-iT®
Plus EdU Alexa Fluor®
647 Flow Cytometry Assay Kit *100 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
A10044 EdU (5-ethynyl-2´-deoxyuridine) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mg
B35129 BrdU mouse monoclonal antibody (Clone MoBU-1) - Pacific Blue™
*for flow cytometry* *100 tests* . . . . . . . . . . . . . . . . . . . . 1 each
B35139 BrdU mouse monoclonal antibody (Clone MoBU-1) Alexa Fluor®
488 *for flow cytometry* *100 tests* . . . . . . . . . . . . . . . . . . . 1 each
B35140 BrdU mouse monoclonal antibody (Clone MoBU-1) Alexa Fluor®
647 *for flow cytometry* *100 tests* . . . . . . . . . . . . . . . . . . . 1 each
B35141 BrdU mouse monoclonal antibody (Clone MoBU-1) unconjugated *for flow cytometry* *100 tests*  . . . . . . . . . . . . . . . . . . . . . 1 each
F10347 FxCycle™
Violet Stain *for flow cytometry* *500 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
F10348 FxCycle™
Far Red Stain *for flow cytometry* *500 assays*  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
F10797 FxCycle™
PI/RNase Staining Solution *200 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 mL
H3570 Hoechst®
33342, trihydrochloride, trihydrate *10 mg/mL solution in water* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mL
P3566 Propidium Iodide – 1.0mg/ml solution in water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mL
S10349 SYTOX®
AADvanced™
dead cell stain *for 488 excitation* *for flow cytometry* *100 tests*  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 kit
V35003 Vybrant®
DyeCycle™
Violet stain *5 mM in water* *200 assays* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200 µL
12091-021 RNase A (20 mg/mL) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 mL
14190-144 Dulbecco’s Phosphate Buffered Saline 1X without Calcium Chloride without Magnesium Chloride . . . . . . . . . . . . . . . . . . . . . . 500 mL
14190-250 Dulbecco’s Phosphate Buffered Saline 1X without Calcium Chloride without Magnesium Chloride . . . . . . . . . . . . . . . . . . . 10 x 500 mL
Purchaser notification
Corporate headquarters
5791 Van Allen Way
Carlsbad, CA 92008
USA
Phone: +1 760 603 7200
Fax: +1 760 602 6500
Email: techsupport@lifetech.com
European headquarters
Inchinnan Business Park
3 Fountain Drive
Paisley PA4 9RF
UK
Phone: +44 141 814 6100
Toll-Free Phone: 0800 269 210
Toll-Free Tech: 0800 838 380
Fax: +44 141 814 6260
Tech Fax: +44 141 814 6117
Email: euroinfo@invitrogen.com
Email Tech: eurotech@invitrogen.com
Japanese headquarters
LOOP-X Bldg. 6F
3-9-15, Kaigan
Minato-ku, Tokyo 108-0022
Japan
Phone: +81 3 5730 6509
Fax: +81 3 5730 6519
Email: jpinfo@invitrogen.com
Additional international offices are listed at
www.lifetechnologies.com
These high-quality reagents and materials must be used by, or directl­y under the super­vision of, a tech­nically qualified individual
experienced in handling potentially hazardous chemicals. Read the Safety Data Sheet provided for each product; other
regulatory considerations may apply.
Obtaining support
For the latest services and support information for all locations, go to www.lifetechnologies.com.
At the website, you can:
•	 Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities
•	 Search through frequently asked questions (FAQs)
•	 Submit a question directly to Technical Support (techsupport@lifetech.com)
•	 Search for user documents, SDSs, vector maps and sequences, application notes, formulations, handbooks, certificates of analysis,
citations, and other product support documents
•	 Obtain information about customer training
•	 Download software updates and patches
SDS
Safety Data Sheets (SDSs) are available at www.lifetechnologies.com/sds.
Certificate of Analysis
The Certificate of Analysis provides detailed quality control and product qualification information for each product. Certificates
of Analysis are available on our website. Go to www.lifetechnologies.com/support and search for the Certificate of Analysis by
product lot number, which is printed on the product packaging (tube, pouch, or box).
Limited product warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms
and Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any
questions, please contact Life Technologies at www.lifetechnologies.com/support.
Disclaimer
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS
DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR
A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE
TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE
OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN
CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Important licensing information
These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and
conditions of all applicable Limited Use Label Licenses.
Hoechst is a registered trademark of Hoechst GmbH. All other trademarks are the property of Thermo Fisher Scientific and its
subsidiaries.
Life Technologies is a Thermo Fisher Scientific brand. © 2014 Thermo Fisher Scientific Inc. All rights reserved.
4 February 2014