CD11b : PerCP - Area+ 75,3 CD11b : PerCP - Area- 24,7 % neu 59,6 FSC-A :: FSC - Area x SSC-A :: SSC - Area % compact cells 97,0 FSC-A :: FSC - Area x SSC-A :: SSC - Area % singlets 97,1 FSC-A :: FSC - Area x FSC-H :: FSC - Height 0 10 3 10 4 10 5 10 6 PerCP-A :: CD11b : PerCP - Area 0 500 1,0K 1,5K Count 004 3A farbena GCSF WLSM.fcs % neu 56038 T cytotoxic 42,5 T helper 49,6 Q4 40,0 Q2 0,13 B lymph 5,83 T lymph 54,1 APC-A :: CD19 : APC - Area x PE-A :: CD3 : PE - Area % lymph 27,8 FSC-A :: FSC - Area x SSC-A :: SSC - Area % compact cells 96,9 FSC-A :: FSC - Area x SSC-A :: SSC - Area % singlets 99,3 FSC-A :: FSC - Area x FSC-H :: FSC - Height 0-10 3 10 3 10 4 10 5 10 6 APC-Cy7-A :: CD8 : APC-Cy7 - Area 0 -10 3 10 3 10 4 10 5 10 6Pe_Cy7-A::CD4:Pe_Cy7-Area 002 3B farbena NT WLSM.fcs T lymph 14459 CD11b : PerCP - Area+ 75,3 CD11b : PerCP - Area- 24,7 0 10 3 10 4 10 5 10 6 PerCP-A :: CD11b : PerCP - Area 0 1,0K 2,0K 3,0K 4,0K Count Sample Name Subset Name Count 005 2 iso ctrl WLSM.fcs % compact cells 96557 002 3B farbena NT WLSM.fcs % neu 65065 004 3A farbena GCSF WLSM.fcs % neu 56038 Q1 0,19 Q2 0,040 Q3 0,32 Q4 99,4 0 10 4 10 5 10 6 APC-A :: CD19 : APC - Area 0 -10 3 10 3 10 4 10 5 10 6 PE-A::CD3:PE-Area 005 2 iso ctrl WLSM.fcs % compact cells 96557 Gating T populacii a B lymfocytu Isotypová ctrl Marker aktivacie neu v porovnani vzorku s GCSF a bez GCSF + isotypova ctrl Gating neutrofilu vcetne markeru aktivacie