CD11b : PerCP - Area+ 86,1 CD11b : PerCP - Area- 13,9 % neu 20,1 FSC-A :: FSC - Area x SSC-A :: SSC - Area % compact cells 86,1 FSC-A :: FSC - Area x SSC-A :: SSC - Area % singlets 93,6 FSC-A :: FSC - Area x FSC-H :: FSC - Height 0 10 4 10 5 10 6 PerCP-A :: CD11b : PerCP - Area 0 100 200 300 400 500 Count 005 3A farbena GCSF WLSM.fcs % neu 16240 B lymph 0,19 Q6 0,22 Q8 99,4 T lymph 0,15 0-10 4 10 4 10 5 10 6 APC-A :: CD19 : APC - Area 0 10 4 10 5 10 6 10 7 10 8 PE-A::CD3:PE-Area 002 2 iso WLSM.fcs % compact cells 81049 Gating T populacii a B lymfocytu Marker aktivacie neu v porovnani vzorku s GCSF a bez GCSF + isotypova ctrl Gating neutrofilu vcetne markeru aktivacie Isotypová ctrl T cytotoxic 34,7 T helper 61,7 Q8 22,5 Q6 0,41 B lymph 6,22 T lymph 70,8 APC-A :: CD19 : APC - Area x PE-A :: CD3 : PE - Area % lymph 63,9 FSC-A :: FSC - Area x SSC-A :: SSC - Area % compact cells 82,9 FSC-A :: FSC - Area x SSC-A :: SSC - Area % singlets 98,3 FSC-A :: FSC - Area x FSC-H :: FSC - Height 0 10 4 10 6 10 8 APC-Cy7-A :: CD8 : APC-Cy7 - Area 0 10 4 10 5 10 6 10 7 10 8 Pe_Cy7-A::CD4:Pe_Cy7-Area 003 3B farbena NT WLSM.fcs T lymph 36892 CD11b : PerCP - Area+ 50,6 CD11b : PerCP - Area- 49,4 0 10 4 10 5 10 6 PerCP-A :: CD11b : PerCP - Area 0 1,0K 2,0K 3,0K Count Sample Name Subset Name Count 002 2 iso WLSM.fcs % compact cells 81049 005 3A farbena GCSF WLSM.fcs % neu 16240 003 3B farbena NT WLSM.fcs % neu 21655