celi_cvika_DNA_diagnostika_2021 Experiment Creation Date 11/22/2021 10:32:51 AM Last Modified Date 11/22/2021 12:54:39 PM Operator Operator Owner Operator Start Time 11/22/2021 10:34:21 AM End Time 11/22/2021 11:42:12 AM Run State Completed Software Version LCS480 1.5.1.62 Macro Macro Owner Macro Status Templates Celiake Plate ID Test ID Lot ID Color Comp ID Run Notes Programs Program Name Program Cycles Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per°C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:03:00 4.40 0 0 0 Program Name Cycles Program 40 Analysis Mode Quantification Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per°C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:00:15 4.40 0 0 0 58 Single 00:00:40 2.20 0 0 0 Program Name Program Cycles 1 Analysis Mode | None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per°C) Sec Target (°C) Step size (°C) Step Delay (cycles) 40 None 00:01:00 2.20 kanál 1 (Abs Quant/2nd Derivative Max) Results Inc Pos Name Type CP Concentration Standard Status 0 A1 A1 Unknown 29.21 0 A2 A2 Unknown 30.76 0 A3 A3 Unknown 29.75 ? - Detector Call uncertain celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 1 of 6 Results Inc Pos Name Type CP Concentration Standard Status 0 A4 A4 Unknown 28.95 0 A5 A5 Unknown 0 A6 A6 Unknown 0 A7 A7 Unknown 0 A8 A8 Unknown 0 A9 A9 Unknown 0 A10 A10 Unknown 0 A11 A11 Unknown 0 A12 A12 Unknown 0 B1 B1 Unknown 29.77 0 B2 B2 Unknown 0 B3 B3 Unknown 31.34 ? 0 B4 B4 Unknown 0 B5 B5 Unknown 0 B6 B6 Unknown 0 B7 B7 Unknown 0 B8 B8 Unknown 31.51 ? 0 B9 B9 Unknown 29.79 0 B10 B10 Unknown 28.56 0 B11 B11 Unknown 34.05 0 B12 B12 Unknown 31.87 0 C1 C1 Unknown 0 C2 C2 Unknown 0 C3 C3 Unknown 0 C4 C4 Unknown 32.74 0 C5 C5 Unknown 27.97 0 C6 C6 Unknown 26.97 0 C7 C7 Unknown 0 C8 C8 Unknown 29.24 0 C9 C9 Unknown 0 C10 C10 Unknown 29.11 0 C11 C11 Unknown 0 C12 C12 Unknown 0 D1 D1 Unknown 0 D2 D2 Unknown 0 D3 D3 Unknown 0 D4 D4 Unknown ? - Detector Call uncertain celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 2 of 6 Results Inc Pos Name Type CP Concentration Standard Status 0 D5 D5 Unknown 28.51 0 D6 D6 Unknown 0 D7 D7 Unknown 0 D8 D8 Unknown 28.96 0 D9 D9 Unknown 0 D10 D10 Unknown 29.08 0 D11 D11 Unknown 0 D12 D12 Unknown 0 E1 E1 Unknown 30.26 0 E2 E2 Unknown 28.18 0 E3 E3 Unknown 0 F1 F1 Unknown 32.66 0 F2 F2 Unknown 0 F3 F3 Unknown 0 F12 pcDQ2 Unknown 25.76 0 G1 G1 Unknown 30.09 0 G2 G2 Unknown 0 G3 G3 Unknown 0 G12 pcDQ8 Unknown 24.62 0 H12 pcDR4 Unknown 27.79 ? - Detector Call uncertain celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 3 of 6 Amplification Curves — A1: A1 — A2: A2 — A3: A3 — A4: A4 — AS: A5 A6: A6 — A7: A7 - A3 A3 — A9: A9 - A1 Q: A10 — A11: A11 - A12: A12 — B1: B1 — B2: B2 - B3: B3 — B4: B4 - B5: B5 B6: B6 — B7: B7 BS: BS — B9: B9 - B1 0: B10 — B11: B11 - B12: B12 — G1: C1 — C2: C2 - C3: C3 — C4: C4 — C5:C5 - C6 ce — CT. CT - CB: CS, — C9: C9 -C10: CID — C11: C11 C12: C12 — D1: D1 — D2: D2 -03: D3 — D4D4 -D5: 05 - 06:06 — D7: D7 DE: DE — 09:09 -010: 010 — D11: D11 -012: 012 — E1: E1 — E2: E2 — E3:E3 — F1: F1 — F2: F2 — F3: F3 - F12: pcDQ2 — G1: G1 — G2:G2 — G3: G3 G12: pcDQB - H12: pcDR4 84.52G: 76.52£ Š G8.52£ ^ G0.52G I 52.52£ % 44.52&Í í 3G.52&Í I 28.52GÍ J 20.52GÍ 12.52GÍ 4.52GÍ ~i-1-i-i-1-1-r~ 5 10 15 20 25 30 35 Cycles kanál 2 (Abs Quant/2nd Derivative Max) Results Inc Pos Name Type CP Concentration Standard Status 0 A1 A1 Unknown 0 A2 A2 Unknown 29.33 0 A3 A3 Unknown 28.98 0 A4 A4 Unknown 29.53 0 A5 A5 Unknown 0 A6 A6 Unknown 30.71 0 A7 A7 Unknown 0 A8 A8 Unknown 0 A9 A9 Unknown 30.84 0 A10 A10 Unknown 0 A11 A11 Unknown 0 A12 A12 Unknown 0 B1 B1 Unknown 29.33 0 B2 B2 Unknown 0 B3 B3 Unknown 28.87 0 B4 B4 Unknown 0 B5 B5 Unknown celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 4 of 6 Results Inc Pos Name Type CP Concentration Standard Status 0 B6 B6 Unknown 28.17 0 B7 B7 Unknown 29.54 0 B8 B8 Unknown 0 B9 B9 Unknown 0 B10 B10 Unknown 29.15 0 B11 B11 Unknown 0 B12 B12 Unknown 31.89 0 C1 C1 Unknown 0 C2 C2 Unknown 0 C3 C3 Unknown 28.56 0 C4 C4 Unknown 30.97 0 C5 C5 Unknown 27.23 0 C6 C6 Unknown 0 C7 C7 Unknown 29.93 0 C8 C8 Unknown 27.89 0 C9 C9 Unknown 30.47 0 C10 C10 Unknown 29.79 0 C11 C11 Unknown 0 C12 C12 Unknown 0 D1 D1 Unknown 30.49 0 D2 D2 Unknown 26.88 0 D3 D3 Unknown 0 D4 D4 Unknown 30.65 0 D5 D5 Unknown 27.07 0 D6 D6 Unknown 0 D7 D7 Unknown 0 D8 D8 Unknown 27.45 0 D9 D9 Unknown 0 D10 D10 Unknown 29.86 0 D11 D11 Unknown 0 D12 D12 Unknown 32.38 0 E1 E1 Unknown 0 E2 E2 Unknown 0 E3 E3 Unknown 0 F1 F1 Unknown 30.50 0 F2 F2 Unknown 0 F3 F3 Unknown celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 5 of 6 Results Inc Pos Name Type CP Concentration Standard Status 0 F12 pcDQ2 Unknown 25.68 0 G1 G1 Unknown 27.17 0 G2 G2 Unknown 29.16 0 G3 G3 Unknown 30.54 0 G12 pcDQ8 Unknown 23.41 0 H12 pcDR4 Unknown 25.04 Amplification Curves — A1: A1 — A2: A2 — A3: A3 — A4: A4 — AS: A5 A6: A6 — A7: A7 - AS A3 — A9: A9 — A10: A1D — A11: A11 - A12: A12 — B1: B1 — B2: B2 - B3: B3 — B4: B4 - B5: B5 B6: B6 — B7: B7 BE: BE — B9: B9 - B1Q; B1D — B11: B11 - B12: B12 — G1: C1 — C2: C2 - C3: C3 — C4: C4 — C5:C5 - C6 C6 — CT. CT - CB: CS, — C9: C9 -C10: C1Q — C11: C11 -C12: C12 — D1: D1 — 02:02 -03:03 — D4D4 -05:05 06:06 — D7: D7 DE: DE — D9D9 -010: 010 — D11: D11 -012: 012 — E1: E1 — E2: E2 — E3:E3 — F1: F1 — F2: F2 — F3: F3 - F12: pcDQ2 — G1: G1 — G2:G2 — G3: G3 G12: pcDQB - H12: pcDR4 celi_cvika_DNA_diagnostika_2021 11/22/2021 Page 6 of 6