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LightCycIer® 480 Software
' IIIIIIIlliF
Report_ ■■■■■■■■■■er
<Ročhe)
co vi d_c vi ka_moco vky_2021 Experiment
Creation Date	11/8/2021 10:29:53 AM	Last Modified Date	11/8/2021 12:03:10 PM
Operator	Operator	Owner	Operator
Start Time	11/8/2021 10:33:47 AM	End Time	11/8/2021 11:44:42 AM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	močovky	Plate ID	
Test ID		Lot ID	
Color Comp ID			
Run Notes			
Programs
Program Name		Program							
Cycles			Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:03:00	4.40		0	0	0
Program Name Cycles		Program							
		50	Analysis Mode		Quantification				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:05	4.40		0	0	0
	55	Single		00:00:20	2.20		0	0	0
	72	None		00:00:10	4.40		0	0	0
Program Name		Program							
Cycles		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	40	None		00:01:00	2.20		0	0	0
FAM (Abs Quant/2nd Derivative Max)
covid_cvika_mocovky_2021
11/8/2021
Page 1 of 5
Settings
Channel	465-510		
Color Compensation	4colorHydrolysis_05042018 (CC) [465-510,533-580]		
Program	Program	Units	
Mode	High Confidence		
Subset Name mocovy2021
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	A1	Unknown				
0	A2	A2	Unknown				
0	A3	A3	Unknown				
0	A4	A4	Unknown				
0	A5	A5	Unknown				
0	A6	A6	Unknown	34.71			
0	A7	A7	Unknown	30.34			
0	A8	A8	Unknown	23.55			
0	A9	A9	Unknown				
0	A10	A10	Unknown	32.43			
0	A11	A11	Unknown	33.89			
0	A12	A12	Unknown				
0	B1	B1	Unknown	24.20			
0	B2	B2	Unknown	34.04			?
0	B3	B3	Unknown				
0	B4	B4	Unknown	34.48			
0	B5	B5	Unknown	20.39			
0	B6	B6	Unknown	22.77			
0	B7	B7	Unknown	34.50			
0	B8	B8	Unknown				
0	B9	B9	Unknown				
0	B10	B10	Unknown	30.50			
0	B11	B11	Unknown				
0	B12	B12	Unknown	20.08			
0	C1	C1	Unknown				
0	C2	C2	Unknown	35.29			
0	C3	C3	Unknown				
0	C4	C4	Unknown	33.78			
0	C5	C5	Unknown				
0	C6	C6	Unknown	24.30			
? - Detector Call uncertain
covid_cvika_mocovky_2021
11/8/2021
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	C7	C7	Unknown	34.37			
0	C8	C8	Unknown	34.69			
0	C9	C9	Unknown				
0	C10	C10	Unknown				
0	C11	C11	Unknown	34.11			
0	C12	C12	Unknown				
0	D1	D1	Unknown	33.66			?
0	D2	D2	Unknown				
0	H11	pcG	Unknown	27.22			
0	H12	pcU	Unknown	25.89			?
? - Detector Call uncertain
Amplification Curves
- A1: A1	— A2: A2	— A3: A3	— A4: A4	— A3: A5	A6: A6	— A7: A7	Aß: Aß	— A9: A9
- A1□: A10	— A11: A11	- A12: A12	— B1: B1	— B2: B2	- B3: B3	— B4: B4	- 85: BS	B6: B6
— B7: B7	— B3: B3	— B9: B9	- B1Q: B1Q	— B11:B11	- B12: B12	— C1:C1	— C2: C2	— C3: C3
— ca. a	-CS: C5	-C6: 06	— C7: 07	-CS: CS	— C9C9	- C1G:C10	— 011 C11	C12: C12
— Di; D1	— D2: D2	— H11: pcG	- H12: pcU					
80.250-73.25CH 66.250: £ 59.25CH " 52.25CH 45.25CH
i 38.25CH I 31.25CH J 24.25CH "* 17.25CH 10.25CH 3.25CH
1 5	10            15           20            25 30 Cycles	35	40	i 45	50
HEX (Abs Quant/2nd Derivative Max) Settings					
Channel	533-580				
Color Compensation	4colorHydrolysis_05042018 (CC) [465-510,533-580]				
Program	Program			Units	
Mode	High Confidence				
Subset Name mocovy2021
1
covid_cvika_mocovky_2021
11/8/2021
Page 3 of 5
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	A1	Unknown				
0	A2	A2	Unknown				
0	A3	A3	Unknown				
0	A4	A4	Unknown				
0	A5	A5	Unknown				
0	A6	A6	Unknown	32.54			
0	A7	A7	Unknown	31.99			
0	A8	A8	Unknown				
0	A9	A9	Unknown	31.30			
0	A10	A10	Unknown	32.76			
0	A11	A11	Unknown				
0	A12	A12	Unknown				
0	B1	B1	Unknown				
0	B2	B2	Unknown	31.46			
0	B3	B3	Unknown				
0	B4	B4	Unknown	31.68			?
0	B5	B5	Unknown				
0	B6	B6	Unknown				
0	B7	B7	Unknown				
0	B8	B8	Unknown				
0	B9	B9	Unknown				
0	B10	B10	Unknown	31.84			
0	B11	B11	Unknown				
0	B12	B12	Unknown				
0	C1	C1	Unknown				
0	C2	C2	Unknown	31.60			
0	C3	C3	Unknown				
0	C4	C4	Unknown	31.51			
0	C5	C5	Unknown				
0	C6	C6	Unknown				
0	C7	C7	Unknown				
0	C8	C8	Unknown	31.01			?
0	C9	C9	Unknown				
0	C10	C10	Unknown				
0	C11	C11	Unknown	31.57			?
0	C12	C12	Unknown				
0	D1	D1	Unknown	31.67			?
? - Detector Call uncertain
covid_cvika_mocovky_2021
11/8/2021
Page 4 of 5
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	D2	D2	Unknown				
0	H11	pcG	Unknown				
0	H12	pcU	Unknown				
? - Detector Call uncertain
Amplification Curves
— A1: A1	— A2: A2	— A'i: A3	— A4: A4	— A5: A5	A6: A6	— A7: A7	Aß: AB	— A&: A9
- A1q: A10	— A11: A11	- A12: A12	— B1: B1	— B2: B2	- B3: BS	— B4: B4	- 85: B5	- B6:B6
— B7: B7	- Bö: BS	— B9: B9	- B10: B10	— B11:B11	- B12: B12	— C1:C1	— C2: C2	— C3: C3
— ca. a	-CS: CS	-06: 06	— CT: Cl	— CS: CS	— C9 C9	- C1G:C10	— 011 011	C12: C12
- D1: D1	— D2: 02	— H11: pcG	- H12: pcU					
25 30 Cycles
covid_cvika_mocovky_2021
11/8/2021
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