Task 1 14-Oct-20
Determination of oligonucleotide concentration
You have obtained envelope from your favorite supplier. The envelope contained dried
primer with sequence 5´- gTAAAACgACggCCAgT -3´.
The synthesis protocol says that the total amount of DNA is approximately 10 µg.
After dissolving the whole amount in 1 mL of TE buffer, in cuvette with optical length 1cm,
you measured exact absorbance A at 260 nm.
1) What is the precise molar concentration of DNA in units µM (10-6
M)?
2) How much was the light intensity reduced after going through the cuvette if compared
with the intensity of incident light? Report the transmitted light intensity in percent of
the original incident light intensity.
http://www.molbiotools.com/dnacalculator.html
Please select „single strand“ in the parameters.
Absorbance values A are listed by your name below.
Please send me your short answers via email within 48 hours.
Correct answer = 1 point.
A A
1 Dikunová Alžbeta 0,300 111 Kubinyiová Lenka 0,450
2 Dzurov Matej 0,590 12 Kůřilová Eliška 0,465
3 Faturová Jana 0,315 13 Lysáková Klára 0,585
4 Gašparik Norbert 0,330 14 Mikšátková Barbora 0,480
5 Hesko Ondrej 0,360 15 Nováková Barbora 0,495
6 Jahodová Kateřina 0,375 16
Prabhullachandran
Unnikannan
0,525
7 Kameniarová Michaela 0,390 17 Procházková Markéta 0,540
8 Konečná Kateřina 0,405 18 Šimek Jan 0,555
9 Korytářová Anna 0,420 19 Tužinčin Dávid 0,570
10 Kozeleková Aneta 0,435