QIAGEN www.qiagen.com Quantitation Report Experiment Information Run Name Run 2021-12-15-sk1_ACO3 Run Start 15.12.2021 10:53:27 Run Finish 15.12.2021 12:30:12 Operator MP Notes Run On Software Version Rotor-Gene Q Software 2.0.2.4 Run Signature The Run Signature is valid. Gain Green 10, Machine Serial No. 0911113 Quantitation Information Threshold 0,030 Left Threshold 5,000 Standard Curve Imported No Standard Curve (1) conc= 10^(-0,345*CT + 9,177) Standard Curve (2) CT = -2,899*log(conc) + 26,608 Reaction efficiency (*) (* = 10^(-1/m) - 1) 1,21265 M -2,89929 B 26,608 R Value 0,99608 R^2 Value 0,99218 Start normalising from cycle 1 Noise Slope Correction Yes No Template Control Threshold % 0 Reaction Efficiency Threshold Disabled Normalisation Method Dynamic Tube Normalisation Digital Filter Light Sample Page Page 2 Imported Analysis Settings Profile Cycle Cycle Point Hold 1 Hold @ 95°C, 10min 0s Cycling (35 repeats) Step 1: Hold @ 95°C, 15s Step 2: Hold @ 60°C, 30s, acquiring to Cycling A([Green][1][1]) Step 3: Hold @ 72°C, 20s Hold 2 Hold @ 72°C, 1min 0s Melt Ramp from 55°C to 90°C Hold for 90s on the 1st step Hold for 5s on next steps,Melt A([Green][1][1]) Raw Data For Cycling A.Green Quantitation data for Cycling A.Green Standard Curve No. Color Name Type Ct Ct Comment Given Conc (IU/ml) Calc Conc (IU/ml) 1 T6 1 1X Standard 15,01 10 000 10 040 2 T6 1 1X Standard 14,96 10 000 10 387 3 T6 1 10x Standard 17,40 1 000 1 504 4 T6 1 10x Standard 17,66 1 000 1 216 5 T6 1 100x Standard 21,47 100 59 6 T6 1 100x Standard 21,10 100 80 7 T6 1 1000x Standard 23,98 10 8 8 T6 1 1000x Standard 24,04 10 8 9 T6 1 10000x Standard 26,11 1 1 10 T6 1 10000x Standard 26,37 1 1 11 1 NK Unknown 29,54 12 1 NK Unknown 27,71 13 D6 1 ACO3 Unknown 15,83 5 228 14 D6 1 ACO3 Unknown 15,06 9 604 15 D6 1 ACO3 Unknown 15,07 9 542 16 B6 1 ACO3 Unknown 14,06 21 360 17 B6 1 ACO3 Unknown 14,61 13 790 18 B6 1 ACO3 Unknown 14,09 20 804 19 1 NK Unknown 28,90 20 1 NK Unknown 31,06 - Warning: The following samples were not analysed : 21T6 2 10X- 22T6 2 10X- 23T6 2 1x- 24T6 2 1x- 25T6 2 100x- 26T6 2 100x- 27T6 2 1000x- 28T6 2 1000x- 29T6 2 10000x- 30T6 2 10000x- 312 NK- 322 NK- 33D6 2 ACO2- 34D6 2 ACO2- 35D6 2 ACO2- 362 NK- 37B6 2 ACO2- 38B6 2 ACO2- 39B6 2 ACO2- 402 NK- 41T6 3 1X- 42T6 3 1X- 43T6 3 10x- 44T6 3 10x- 45T6 3 100x- 46T6 3 100x- 47T6 3 1000x- 48T6 3 1000x- 49T6 3 10000x- 50T6 3 10000x- 513 NK- 523 NK- 53D6 3 ACO4- 54D6 3 ACO4- 55D6 3 ACO4- 563 NK- 57B6 3 ACO4- 58B6 3 ACO4- 59B6 3 ACO4- 603 NK Legend: NEG (NTC) - Sample cancelled due to NTC Threshold. NEG (R. Eff) - Sample cancelled as efficiency less than reaction efficiency threshold. This report was generated by Rotor-Gene Q Series Software 2.3.1 (Build 49) Copyright 2013 QIAGEN GmbH. All Rights Reserved.