QIAGEN www.qiagen.com Quantitation Report Experiment Information Run Name Run 2021-12-15-sk2 Run Start 15.12.2021 10:53:27 Run Finish 15.12.2021 12:30:12 Operator MP Notes Run On Software Version Rotor-Gene Q Software 2.0.2.4 Run Signature The Run Signature is valid. Gain Green 10, Machine Serial No. 0911113 Quantitation Information Threshold 0,030 Left Threshold 5,000 Standard Curve Imported No Standard Curve (1) conc= 10^(-0,513*CT + 13,442) Standard Curve (2) CT = -1,950*log(conc) + 26,215 Reaction efficiency (*) (* = 10^(-1/m) - 1) 2,25638 M -1,95033 B 26,21531 R Value 0,96098 R^2 Value 0,92348 Start normalising from cycle 1 Noise Slope Correction Yes No Template Control Threshold % 0 Reaction Efficiency Threshold Disabled Normalisation Method Dynamic Tube Normalisation Digital Filter Light Sample Page Page 2 Imported Analysis Settings Profile Cycle Cycle Point Hold 1 Hold @ 95°C, 10min 0s Cycling (35 repeats) Step 1: Hold @ 95°C, 15s Step 2: Hold @ 60°C, 30s, acquiring to Cycling A([Green][1][1]) Step 3: Hold @ 72°C, 20s Hold 2 Hold @ 72°C, 1min 0s Melt Ramp from 55°C to 90°C Hold for 90s on the 1st step Hold for 5s on next steps,Melt A([Green][1][1]) Raw Data For Cycling A.Green Quantitation data for Cycling A.Green Standard Curve No. Color Name Type Ct Ct Comment Given Conc (IU/ml) Calc Conc (IU/ml) 21 T6 2 10X Standard 21,17 1 000 385 22 T6 2 10X Standard 21,23 1 000 359 23 T6 2 1x Standard 18,70 10 000 7 170 24 T6 2 1x Standard 18,36 10 000 10 710 25 T6 2 100x Standard 20,93 100 515 26 T6 2 100x Standard 21,06 100 442 27 T6 2 1000x Standard 23,89 10 16 28 T6 2 1000x Standard 24,01 10 13 29 T6 2 10000x Standard 27,18 1 30 T6 2 10000x Standard 26,63 1 1 31 2 NK Unknown 27,17 32 2 NK Unknown 26,32 1 33 D6 2 ACO2 Unknown 32,81 34 D6 2 ACO2 Unknown 29,12 35 D6 2 ACO2 Unknown 24,55 7 36 2 NK Unknown 27,16 37 B6 2 ACO2 Unknown 14,58 925 002 38 B6 2 ACO2 Unknown 14,58 929 261 39 B6 2 ACO2 Unknown 14,43 1 101 722 - Warning: The following samples were not analysed : 1T6 1 1X- 2T6 1 1X- 3T6 1 10x- 4T6 1 10x- 5T6 1 100x- 6T6 1 100x- 7T6 1 1000x- 8T6 1 1000x- 9T6 1 10000x- 10T6 1 10000x- 111 NK- 121 NK- 13D6 1 ACO3- 14D6 1 ACO3- 15D6 1 ACO3- 16B6 1 ACO3- 17B6 1 ACO3- 18B6 1 ACO3- 191 NK- 201 NK- 402 NK- 41T6 3 1X- 42T6 3 1X- 43T6 3 10x- 44T6 3 10x- 45T6 3 100x- 46T6 3 100x- 47T6 3 1000x- 48T6 3 1000x- 49T6 3 10000x- 50T6 3 10000x- 513 NK- 523 NK- 53D6 3 ACO4- 54D6 3 ACO4- 55D6 3 ACO4- 563 NK- 57B6 3 ACO4- 58B6 3 ACO4- 59B6 3 ACO4- 603 NK Legend: NEG (NTC) - Sample cancelled due to NTC Threshold. NEG (R. Eff) - Sample cancelled as efficiency less than reaction efficiency threshold. This report was generated by Rotor-Gene Q Series Software 2.3.1 (Build 49) Copyright 2013 QIAGEN GmbH. All Rights Reserved.