QIAGEN www.qiagen.com Quantitation Report Experiment Information Run Name Run 2021-12-15-sk1_ACO3 Run Start 15.12.2021 10:53:27 Run Finish 15.12.2021 12:30:12 Operator MP Notes Run On Software Version Rotor-Gene Q Software 2.0.2.4 Run Signature The Run Signature is valid. Gain Green 10, Machine Serial No. 0911113 Quantitation Information Threshold 0,030 Left Threshold 5,000 Standard Curve Imported No Standard Curve (1) conc= 10^(-0,360*CT + 10,759) Standard Curve (2) CT = -2,779*log(conc) + 29,898 Reaction efficiency (*) (* = 10^(-1/m) - 1) 1,29001 M -2,77904 B 29,89843 R Value 0,99501 R^2 Value 0,99004 Start normalising from cycle 1 Noise Slope Correction Yes No Template Control Threshold % 0 Reaction Efficiency Threshold Disabled Normalisation Method Dynamic Tube Normalisation Digital Filter Light Sample Page Page 2 Imported Analysis Settings Profile Cycle Cycle Point Hold 1 Hold @ 95°C, 10min 0s Cycling (35 repeats) Step 1: Hold @ 95°C, 15s Step 2: Hold @ 60°C, 30s, acquiring to Cycling A([Green][1][1]) Step 3: Hold @ 72°C, 20s Hold 2 Hold @ 72°C, 1min 0s Melt Ramp from 55°C to 90°C Hold for 90s on the 1st step Hold for 5s on next steps,Melt A([Green][1][1]) Raw Data For Cycling A.Green Quantitation data for Cycling A.Green Standard Curve No. Color Name Type Ct Ct Comment Given Conc (IU/ml) Calc Conc (IU/ml) 41 T6 3 1X Standard 18,71 10 000 10 593 42 T6 3 1X Standard 19,37 10 000 6 147 43 T6 3 10x Standard 20,99 1 000 1 603 44 T6 3 10x Standard 21,05 1 000 1 525 45 T6 3 100x Standard 24,83 100 67 47 T6 3 1000x Standard 27,20 10 9 48 T6 3 1000x Standard 27,22 10 9 49 T6 3 10000x Standard 29,79 1 1 51 3 NK Unknown 31,76 52 3 NK Unknown 53 D6 3 ACO4 Unknown 54 D6 3 ACO4 Unknown 55 D6 3 ACO4 Unknown 28,89 2 56 3 NK Unknown 34,85 58 B6 3 ACO4 Unknown 15,87 111 541 59 B6 3 ACO4 Unknown 15,17 199 580 60 3 NK Unknown 29,68 1 - Warning: The following samples were not analysed : 1T6 1 1X- 2T6 1 1X- 3T6 1 10x- 4T6 1 10x- 5T6 1 100x- 6T6 1 100x- 7T6 1 1000x- 8T6 1 1000x- 9T6 1 10000x- 10T6 1 10000x- 111 NK- 121 NK- 13D6 1 ACO3- 14D6 1 ACO3- 15D6 1 ACO3- 16B6 1 ACO3- 17B6 1 ACO3- 18B6 1 ACO3- 191 NK- 201 NK- 21T6 2 10X- 22T6 2 10X- 23T6 2 1x- 24T6 2 1x- 25T6 2 100x- 26T6 2 100x- 27T6 2 1000x- 28T6 2 1000x- 29T6 2 10000x- 30T6 2 10000x- 312 NK- 322 NK- 33D6 2 ACO2- 34D6 2 ACO2- 35D6 2 ACO2- 362 NK- 37B6 2 ACO2- 38B6 2 ACO2- 39B6 2 ACO2- 402 NK- 46T6 3 100x- 50T6 3 10000x- 57B6 3 ACO4 Legend: NEG (NTC) - Sample cancelled due to NTC Threshold. NEG (R. Eff) - Sample cancelled as efficiency less than reaction efficiency threshold. This report was generated by Rotor-Gene Q Series Software 2.3.1 (Build 49) Copyright 2013 QIAGEN GmbH. All Rights Reserved.