QIAGEN www.qiagen.com Quantitation Report Experiment Information Run Name Run 2021-12-15-sk4 Run Start 15.12.2021 13:53:27 Run Finish 15.12.2021 15:40:16 Operator MP Notes Run On Software Version Rotor-Gene Q Software 2.0.2.4 Run Signature The Run Signature is valid. Gain Green 10, Machine Serial No. 0911113 Quantitation Information Threshold 0,030 Left Threshold 5,000 Standard Curve Imported No Standard Curve (1) conc= 10^(-0,462*CT + 13,104) Standard Curve (2) CT = -2,165*log(conc) + 28,364 Reaction efficiency (*) (* = 10^(-1/m) - 1) 1,89724 M -2,16458 B 28,36425 R Value 0,94975 R^2 Value 0,90203 Start normalising from cycle 1 Noise Slope Correction Yes No Template Control Threshold % 0 Reaction Efficiency Threshold Disabled Normalisation Method Dynamic Tube Normalisation Digital Filter Light Sample Page Page 2 Imported Analysis Settings Profile Cycle Cycle Point Hold 1 Hold @ 95°C, 10min 0s Cycling (40 repeats) Step 1: Hold @ 95°C, 15s Step 2: Hold @ 60°C, 30s, acquiring to Cycling A([Green][1][1]) Step 3: Hold @ 72°C, 20s Hold 2 Hold @ 72°C, 1min 0s Melt Ramp from 55°C to 90°C Hold for 90s on the 1st step Hold for 5s on next steps,Melt A([Green][1][1]) Raw Data For Cycling A.Green Quantitation data for Cycling A.Green Standard Curve No. Color Name Type Ct Ct Comment Given Conc (IU/ml) Calc Conc (IU/ml) 1 T6 1X Standard 19,08 10 000 19 419 2 T6 1X Standard 18,96 10 000 22 162 3 T6 10x Standard 21,86 1 000 1 014 4 T6 10x Standard 22,61 1 000 458 5 T6 100x Standard 24,96 100 37 6 T6 100x Standard 25,42 100 23 7 T6 1000x Standard 24,55 10 58 8 T6 1000x Standard 26,55 10 7 9 T6 10000x Standard 29,41 1 10 T6 10000x Standard 26,96 1 4 11 NK Unknown 26,99 4 12 NK Unknown 24,67 51 13 D6 ACO1 Unknown 15,83 614 583 14 D6 ACO1 Unknown 15,39 988 758 15 D6 ACO1 Unknown 15,43 949 130 16 B6 ACO1 Unknown 14,86 1 734 706 17 B6 ACO1 Unknown 14,91 1 637 042 18 B6 ACO1 Unknown 14,82 1 810 215 19 NK Unknown 28,58 1 20 NK Unknown 30,22 - Warning: The following samples were not analysed : 21T6 1X- 22T6 1X- 23T6 10x- 24T6 10x- 25T6 100x- 26T6 100x- 27T6 1000x- 28T6 1000x- 29T6 10000x- 30T6 10000x- 31NK- 32NK- 33D6 ACO5- 34D6 ACO5- 35D6 ACO5- 36B6 ACO5- 37B6 ACO5- 38B6 ACO5- 39NK- 40NK- 41T6 1X- 42T6 1X- 43T6 10x- 44T6 10x- 45T6 100x- 46T6 100x- 47T6 1000x- 48T6 1000x- 49T6 10000x- 50T6 10000x- 51NK- 52NK- 53D6 UBC10- 54D6 UBC10- 55D6 UBC10- 56B6 UBC10- 57B6 UBC10- 58B6 UBC10- 59NK- 60NK Legend: NEG (NTC) - Sample cancelled due to NTC Threshold. NEG (R. Eff) - Sample cancelled as efficiency less than reaction efficiency threshold. This report was generated by Rotor-Gene Q Series Software 2.3.1 (Build 49) Copyright 2013 QIAGEN GmbH. All Rights Reserved.