QIAGEN www.qiagen.com Quantitation Report Experiment Information Run Name Run 2021-12-15-sk6 Run Start 15.12.2021 13:53:27 Run Finish 15.12.2021 15:40:16 Operator MP Notes Run On Software Version Rotor-Gene Q Software 2.0.2.4 Run Signature The Run Signature is valid. Gain Green 10, Machine Serial No. 0911113 Quantitation Information Threshold 0,030 Left Threshold 5,000 Standard Curve Imported No Standard Curve (1) conc= 10^(-0,329*CT + 8,561) Standard Curve (2) CT = -3,037*log(conc) + 25,997 Reaction efficiency (*) (* = 10^(-1/m) - 1) 1,13449 M -3,0368 B 25,99718 R Value 0,97869 R^2 Value 0,95784 Start normalising from cycle 1 Noise Slope Correction Yes No Template Control Threshold % 0 Reaction Efficiency Threshold Disabled Normalisation Method Dynamic Tube Normalisation Digital Filter Light Sample Page Page 2 Imported Analysis Settings Profile Cycle Cycle Point Hold 1 Hold @ 95°C, 10min 0s Cycling (40 repeats) Step 1: Hold @ 95°C, 15s Step 2: Hold @ 60°C, 30s, acquiring to Cycling A([Green][1][1]) Step 3: Hold @ 72°C, 20s Hold 2 Hold @ 72°C, 1min 0s Melt Ramp from 55°C to 90°C Hold for 90s on the 1st step Hold for 5s on next steps,Melt A([Green][1][1]) Raw Data For Cycling A.Green Quantitation data for Cycling A.Green Standard Curve No. Color Name Type Ct Ct Comment Given Conc (IU/ml) Calc Conc (IU/ml) 41 T6 1X Standard 14,70 10 000 5 237 42 T6 1X Standard 14,68 10 000 5 348 43 T6 10x Standard 15,28 1 000 3 394 44 T6 10x Standard 17,16 1 000 811 45 T6 100x Standard 20,08 100 89 46 T6 100x Standard 19,34 100 156 47 T6 1000x Standard 21,90 10 22 48 T6 1000x Standard 22,18 10 18 49 T6 10000x Standard 26,80 1 1 50 T6 10000x Standard 27,13 1 51 NK Unknown 52 NK Unknown 53 D6 UBC10 Unknown 11,94 42 535 54 D6 UBC10 Unknown 12,01 40 427 55 D6 UBC10 Unknown 11,85 45 548 56 B6 UBC10 Unknown 12,70 23 920 57 B6 UBC10 Unknown 12,65 24 923 58 B6 UBC10 Unknown 12,60 25 838 59 NK Unknown 16,16 1 736 60 NK Unknown 21,80 24 - Warning: The following samples were not analysed : 1T6 1X- 2T6 1X- 3T6 10x- 4T6 10x- 5T6 100x- 6T6 100x- 7T6 1000x- 8T6 1000x- 9T6 10000x- 10T6 10000x- 11NK- 12NK- 13D6 ACO1- 14D6 ACO1- 15D6 ACO1- 16B6 ACO1- 17B6 ACO1- 18B6 ACO1- 19NK- 20NK- 21T6 1X- 22T6 1X- 23T6 10x- 24T6 10x- 25T6 100x- 26T6 100x- 27T6 1000x- 28T6 1000x- 29T6 10000x- 30T6 10000x- 31NK- 32NK- 33D6 ACO5- 34D6 ACO5- 35D6 ACO5- 36B6 ACO5- 37B6 ACO5- 38B6 ACO5- 39NK- 40NK Legend: NEG (NTC) - Sample cancelled due to NTC Threshold. NEG (R. Eff) - Sample cancelled as efficiency less than reaction efficiency threshold. This report was generated by Rotor-Gene Q Series Software 2.3.1 (Build 49) Copyright 2013 QIAGEN GmbH. All Rights Reserved.