S2004/S2005 Methods for characterization of biomolecular
interactions – classical vs. modern
Autumn 2021
Teachers: Josef Houser, Monika Kubíčková, Jan Komárek, Eva Fujdiarová,
Michaela Wimmerová
Students enrolled in S2004 course and Workshop participants attend only lectures (L). Students
enrolled in both S2004 and S2005 courses need to attend both lectures (L) and practical exercises (P).
February 1, 2022 (Tuesday)
time program room
09:00-10:30 L Biomolecular interactions – introduction (Wimmerová) C05/114
10:30-10:45 break
10:45-12:15 L Isothermal titration calorimetry (ITC) (Kubíčková) C05/114
12:15-13:15 lunch
13:15-14:15 L Spectroscopic methods (Houser) C05/114
14:15-15:15 L Microscale thermophoresis (MST) (Fujdiarová) C05/114
15:15-15:30 break
15:30-17:00 P ITC – hands-on (Kubíčková) C04/218
February 2, 2022 (Wednesday)
time program room
09:00-10:30 L Analytical ultracentrifugation (AUC) (Komárek) C05/114
10:30-10:45 break
10:45-12:15 L Surface-based methods (Houser) C05/114
12:15-13:15 lunch
13:15-13:45 L Interactions at the cell level (Houser) C05/114
13:45-14:45 L Importance of sample preparation (Houser) C05/114
14:45-15:00 break
15:00-15:45 P ITC – data analysis (Kubíčková) C04/118
15:45-17:30 P AUC – hands-on (Komárek) C04/217
February 3, 2022 (Thursday)
time program room
09:00-10:00 P AUC – data analysis (Komárek) C04/217
10:00-10:15 break
10:15-11:30 P BLI – hands-on (Houser) C04/218
11:30-12:30 lunch
12:30-13:15 P BLI – data analysis (Houser) C04/218
13.15-14.30 P MST – hands-on (Fujdiarová) C04/219
14:30-14:45 break
14:45-15:30 P MST – data analysis (Fujdiarová) C04/219
15:30-16:30 P Discussion of practical aspects (all teachers) C04/218
February 4, 2022 (Friday)
time program room
09:30-11:00 L Written test C04/118