Department of Biophysics
of Immune System
Institute of Biophysics
of the Czech Academy of Sciences
Department structure
Head of the department:
doc. Mgr. Lukáš Kubala, Ph.D.
Scientists:
Mgr. Gabriela Ambrožová, Ph.D.
RNDr. Lenka Šindlerová, Ph.D.
Mgr. Ondřej Vašíček, Ph.D.
Mgr. Jan Víteček, Ph.D.
Mgr. Kristýna Turková, Ph.D.
Ph.D. students
pregradual students
Lukáš Kubala
1. Role of myeloperoxidase (MPO) in the development of
inflammatory and cardiovascular diseases
MPO damages protective layer on the surface of endothelial cells (glycocalyx)
MPO changes viscosity, charge and other properties of glycocalyx components
MPO activates hypertrophy and proliferation of smooth muscle cells => vessel hypertrophy
MPO damages function of cardiac muscle cells (cardiomyocytes)
Lukáš Kubala
2. Significance and function of cAMP in immune cells
• Regulation of specific
and nonspecific
immunity
immunosuppressor
• dominant isoforms
in immune cells
AC7, AC9
• Pharmacological
potentiall:
autoimmune
diseases, chronic
inflammation,
allergies, tumour
diseases
T cells
Treg
B cells
Melanoma
cells
Epithelial
cells
Dendritic
cells
Monocytes
Macrophages
Neutrophils
Eosinophils
Basophils
cAMP
Lukáš Kubala
3. Hyaluronan biology - role of hyaluronan and its receptors
in inflammatory processes
Hyaluronan – key component of extracellular matrix in tissues
Role of low molecular hyaluronan in inflammation regulation, wound
healing, skin regeneration
Product use in clinics and cosmetics
Hyaluronan
Native (high molecular)
Hyaluronan
low molecular, oligosacharides
degradation
inflammation
Stimulates skin regeneration
and wound healing
Active component
of face creams
Active cooperation with biotechnology industry:
Contipro, a.s.
Dolní Dobrouč 401
561 02 Dolní Dobrouč
International cooperation:
Department of Cardiovascular Physiology
Georg-August University Göttingen, Germany
Role of hypoxia in regulation of cardiomycytes physiology
Department of Internal Medicine – Heart centrum
University Hospital of Cologne, Germany
Role of myeloperoxidase in development of cardiovascular diseases
- Haberlea rhodopensis
- Geranium sanguineum
- Macrolepiota procera
Cooperation: Chief Assist. Prof. Eng. Yordan Georgiev, PhD
Ondřej Vašíček - Pharmacological regulation of immune response
1. Study of immunomodulatory effects and biochemical mechanisms of
immunomodulation by heteropolysacharides
Ondřej Vašíček - Pharmacological regulation of immune response
1. Study of immunomodulatory effects and biochemical mechanisms of
immunomodulation by heteropolysacharides
https://natoxaq.ku.dk/toxin-of-the-
week/endotoxins/
Lenka Šindlerová
1. Proinflammatory effects of LPS from cyanobacterial
water bloom
Cooperation with RNDr. Pavel Babica, Ph.D.
from RECETOX MUNI
Heterotrophic Plate Counts (R2A, YEA)
DIN EN ISO 6222
qPCR for total, Gbacteria,
cyanobacteria
16S rDNA NGS
Cyanotoxin
analysis
(LC-MS/MS)
Pyrogene rFC
Sample processing
Net plankton
Lyophilization
LPS isolation
Peterson & Artis 2014
Nature Reviews
Immunology 14; 141-153
Sample processing
Net plankton
Lyophilization
LPS isolation
Differentiated Caco-2 cells
Monocytes from human peripheral blood
• Monocytes differentiation
• Pro-inflammatory cytokines in both compartments
• Changes in permeability of the monolayer
• Changes in TJ proteins expression
Jan Víteček
1. Cell functions (phagocytes and endothelial cells) in
microfluidic conditions
• Long-term cell cultivation in flow conditions
• Development of microfluidic system
Jan Víteček
2. Thrombolysis mechanisms
• Study of thrombolysis using in vitro models
• Static and flow models
• Testing of new thrombolytics
• Cooperation with St. Anne´s University Hospital Brno
Ischemic stroke
Vessel model (middle cerebral artery)
www.strokebrno.com
Gabriela Ambrožová
Bacteria-derived extracellular
vesicles in intestinal inflammation
• Inflammatory Bowel Disease
• uncontrolled activation of intestinal
immune cells in a genetically
susceptible host
• very complex disorder
• biological mechanisms still unknown
• EVs - mediators of intercellular
communication
• produced by both eukaryotic cells and
bacteria
• role of bacteria-derived EVs in IBD
remains unknown Shen, 2021
Gabriela Ambrožová
Bacterial extracellular vesicles in intestinal inflammation
1. Isolation, purification and characterization of bEVs
2. Effects of bEVs on intestinal epithelium
3. Effects of bEVs on immune cells response
4. Revealing of the mechanism responsible
for the effects bEVs in intestinal
inflammation
cryo-EM (Dr. Nováček, CF CEITEC)
Anaerobic chamber
Contacts:
Oddělení Biofyziky imunitního systému
Biofyzikální ústav Akademie věd ČR
Královopolská 135, Brno
Dr. Gabriela Ambrožová ambrozova@ibp.cz
doc. Dr. Lukáš Kubala kubalal@ibp.cz
Dr. Lenka Šindlerová sindler@ibp.cz
Dr. Ondřej Vašíček ondrej.vasicek@ibp.cz
Dr. Jan Víteček jan.vitecek@ibp.cz
https://www.ibp.cz/cs/vyzkum/oddeleni/biofyzika-imunitnich-systemu/informace-o-oddeleni