celi_cvika_2022.pcrd
11/23/2022 16:02
Report Information
User: BioRad/admin
Data File Name: celi_cvika_2022.pcrd
Data File Path: L:\Vysledky CFX96\2022\xxxJiné
Well Group Name: All Wells
Report Differs from Last Save: No
Run Setup
Run Information
Run Date: 11/07/2022 13:59
Run User: admin
Run Type: User-defined
Plate File: FAM-HEX.pltd
ID:
Notes:
Sample Volume: 20
Temperature Control Mode: Calculated
Lid Temperature: 105
Base Serial Number: CT049587
Optical Head Serial Number: 785BR26450
Protocol
1: 95.0°C for 3:00
2: 95.0°C for 0:15
3: 60.0°C for 0:40
Plate Read
4: GOTO 2, 39 more times
5: 40.0°C for 1:00
Plate Display
1 2 3 4 5 6 7 8 9 10 11 12
A Unk
FAM
HEX
ek 2
Unk
FAM
HEX
ek 8
Unk
FAM
HEX
ek 4
Unk
FAM
HEX
vb 2
Unk
FAM
HEX
vb 8
Unk
FAM
HEX
vb 4
Unk
FAM
HEX
zh 2
Unk
FAM
HEX
zh 8
Unk
FAM
HEX
zh 4
Unk
FAM
HEX
tz 2
Unk
FAM
HEX
tz 8
Unk
FAM
HEX
tz 4
B Unk
FAM
HEX
fš 2
Unk
FAM
HEX
fš 8
Unk
FAM
HEX
fš 4
Unk
FAM
HEX
at 2
Unk
FAM
HEX
at 8
Unk
FAM
HEX
at 4
Unk
FAM
HEX
jb 2
Unk
FAM
HEX
jb 8
Unk
FAM
HEX
jb 4
Unk
FAM
HEX
mk 2
Unk
FAM
HEX
mk 8
Unk
FAM
HEX
mk 4
C
D
E
F
G
Plate Display
1 2 3 4 5 6 7 8 9 10 11 12
H Unk
FAM
HEX
pc 2
Unk
FAM
HEX
pc 4
Unk
FAM
HEX
pc 8
Quantification
Step #: 3
Analysis Mode: Fluorophore
Baseline Setting: Baseline Subtracted Curve Fit
Cq Determination: Single Threshold
Baseline Method:
FAM: Auto Calculated
HEX: Auto Calculated
Threshold Setting:
FAM: 468.05, Auto Calculated
HEX: 834.20, Auto Calculated
Quantification Data
Well Fluor Target Content Sample Cq Cq
Mean
Cq
Std.
Dev
A01 FAM Unkn ek 2 27,34 27,34 0
A02 FAM Unkn ek 8 N/A 0 0
A03 FAM Unkn ek 4 N/A 0 0
A04 FAM Unkn vb 2 N/A 0 0
A05 FAM Unkn vb 8 30,01 30,01 0
A06 FAM Unkn vb 4 27,14 27,14 0
A07 FAM Unkn zh 2 30,71 30,71 0
A08 FAM Unkn zh 8 N/A 0 0
A09 FAM Unkn zh 4 28,29 28,29 0
A10 FAM Unkn tz 2 29,1 29,1 0
A11 FAM Unkn tz 8 N/A 0 0
Quantification Data
Well Fluor Target Content Sample Cq Cq
Mean
Cq
Std.
Dev
A12 FAM Unkn tz 4 N/A 0 0
B01 FAM Unkn fš 2 N/A 0 0
B02 FAM Unkn fš 8 N/A 0 0
B03 FAM Unkn fš 4 N/A 0 0
B04 FAM Unkn at 2 N/A 0 0
B05 FAM Unkn at 8 32,17 32,17 0
B06 FAM Unkn at 4 29,66 29,66 0
B07 FAM Unkn jb 2 29,71 29,71 0
B08 FAM Unkn jb 8 N/A 0 0
B09 FAM Unkn jb 4 N/A 0 0
B10 FAM Unkn mk 2 26,7 26,7 0
B11 FAM Unkn mk 8 N/A 0 0
B12 FAM Unkn mk 4 N/A 0 0
H10 FAM Unkn pc 2 24,96 24,96 0
H11 FAM Unkn pc 4 26,05 26,05 0
H12 FAM Unkn pc 8 23,11 23,11 0
A01 HEX Unkn ek 2 30,54 30,54 0
A02 HEX Unkn ek 8 N/A 0 0
A03 HEX Unkn ek 4 28,54 28,54 0
A04 HEX Unkn vb 2 N/A 0 0
A05 HEX Unkn vb 8 27,31 27,31 0
A06 HEX Unkn vb 4 27,02 27,02 0
A07 HEX Unkn zh 2 30,7 30,7 0
A08 HEX Unkn zh 8 28,11 28,11 0
A09 HEX Unkn zh 4 28,41 28,41 0
A10 HEX Unkn tz 2 30,73 30,73 0
A11 HEX Unkn tz 8 N/A 0 0
A12 HEX Unkn tz 4 29,24 29,24 0
B01 HEX Unkn fš 2 31,17 31,17 0
B02 HEX Unkn fš 8 N/A 0 0
B03 HEX Unkn fš 4 30,04 30,04 0
B04 HEX Unkn at 2 N/A 0 0
B05 HEX Unkn at 8 30,14 30,14 0
B06 HEX Unkn at 4 29,04 29,04 0
B07 HEX Unkn jb 2 N/A 0 0
B08 HEX Unkn jb 8 N/A 0 0
B09 HEX Unkn jb 4 29,82 29,82 0
B10 HEX Unkn mk 2 28,95 28,95 0
B11 HEX Unkn mk 8 N/A 0 0
B12 HEX Unkn mk 4 27,32 27,32 0
H10 HEX Unkn pc 2 24,87 24,87 0
H11 HEX Unkn pc 4 24,36 24,36 0
H12 HEX Unkn pc 8 22,29 22,29 0
Bar Chart
Expression analysis requires that at least two wells contain sample names, targets, and valid Cqs.
QC Parameters
Data
Description Value Use Results Exclude
Wells
All
excluded
wells
Negative
control with
a Cq less
than
38 True False
NTC with a
Cq less than
38 True False
NRT with a
Cq less than
38 True False
Positive
control with
a Cq greater
than
30 True False
Unknown
without a
Cq
N/A True FAM:A2,
A3, A4,
A8, A11,
A12, B1,
B2, B3,
B4, B8,
B9, B11,
B12.
HEX:A2,
A4, A11,
B2, B4,
B7, B8,
B11.
False
Standard
without a
Cq
N/A True False
Efficiency
greater than
110.0 True
Efficiency
less than
90.0 True
Std Curve
R^2 less
than
0.980 True
Replicate
group Cq
Std Dev
greater than
0.20 True False