C ID urea_cvika_2022.pcrd 11/05/2022 11:23 Report Information User: BioRad/admin Data File Name: urea_cvika_2022.pcrd Data File Path: L:\Vysledky CFX96\2022 Well Group Name: All Wells Report Differs from Last Save: Yes Run Setup Run Information Run Date: 10/24/2022 15:52 Run User: admin Run Type: User-defined Plate File: FAM-HEX-TexasRed-Cy5.pltd ID: Notes: Sample Volume: 20 Temperature Control Mode: Calculated Lid Temperature: 105 Base Serial Number: CT045679 Optical Head Serial Number: 785BR23781 Protocol 1: 95.0°C for 2:00 2: 95.0°C for 0:10 3: 55.0°C for 0:20 Plate Read 4: 67.0°C for 0:30 5: GOTO 2, 49 more times 6: 40.0°Cfor 1:00 Plate Display 1 2 3 4 5 6 7 8 9 10 11 12 A Unk FAM HEX Texas Red Cy5 M.S. Unk FAM HEX Texas Red Cy5 nc B Unk FAM HEX Texas Red Cy5 EK Unk FAM HEX Texas Red Cy5 pc C Unk FAM HEX Texas Red Cy5 JB Plate Display 1 2 3 4 5 6 7 8 9 10 11 12 D Unk FAM HEX Texas Red Cy5 FS E Unk FAM HEX Texas Red Cy5 AT F Unk FAM HEX Texas Red Cy5 TZ G Unk FAM HEX Texas Red Cy5 VB H Unk FAM HEX Texas Red Cy5 IH Quantification Step #: 3 Analysis Mode: Fluorophore Baseline Setting: Baseline Subtracted Curve Fit Cq Determination: Single Threshold Baseline Method: HEX: Auto Calculated Cy5: Auto Calculated Texas Red: Auto Calculated FAM: Auto Calculated Threshold Setting: HEX: 358.95, Auto Calculated Cy5: 422.33, User Defined Texas Red: 722.03, User Defined FAM: 618.16, User Defined Amplification 16 -f 14 12 4- < o 0 - Quantification Data Well Fluor Target Content Sample Cq Cq Mean Cq Std. Dev A01 Cy5 Unkn M.S. N/A 0 0 A02 Cy5 Unkn nc N/A 0 0 B01 Cy5 Unkn EK N/A 0 0 B02 Cy5 Unkn pc 26,57 26,57 0 C01 Cy5 Unkn JB N/A 0 0 D01 Cy5 Unkn FS N/A 0 0 E01 Cy5 Unkn AT N/A 0 0 F01 Cy5 Unkn TZ 33,85 33,85 0 G01 Cy5 Unkn VB 35,25 35,25 0 HOI Cy5 Unkn IH 23,23 23,23 0 A01 FAM Unkn M.S. N/A 0 0 A02 FAM Unkn nc N/A 0 0 B01 FAM Unkn EK 41,35 41,35 0 B02 FAM Unkn pc 23,47 23,47 0 C01 FAM Unkn JB N/A 0 0 D01 FAM Unkn FS 28,83 28,83 0 E01 FAM Unkn AT 28,03 28,03 0 F01 FAM Unkn TZ N/A 0 0 G01 FAM Unkn VB 45,7 45,7 0 HOI FAM Unkn IH 27,69 27,69 0 A01 HEX Unkn M.S. 31,03 31,03 0 A02 HEX Unkn nc 48,58 48,58 0 B01 HEX Unkn EK 31,25 31,25 0 B02 HEX Unkn pc N/A 0 0 C01 HEX Unkn JB 31 31 0 D01 HEX Unkn FS 32,13 32,13 0 EOl HEX Unkn AT 33,45 33,45 0 FOl HEX Unkn TZ 31,14 31,14 0 Quantification Data Well Fluor Target Content Sample Cq Cq Mean Cq Std. Dev G01 HEX Unkn VB 36,09 36,09 0 HOI HEX Unkn IH 31,74 31,74 0 A01 Texas Red Unkn M.S. N/A 0 0 A02 Texas Red Unkn nc N/A 0 0 B01 Texas Red Unkn EK N/A 0 0 B02 Texas Red Unkn pc 24,04 24,04 0 C01 Texas Red Unkn JB N/A 0 0 D01 Texas Red Unkn FS N/A 0 0 EOl Texas Red Unkn AT N/A 0 0 FOl Texas Red Unkn TZ N/A 0 0 GOl Texas Red Unkn VB N/A 0 0 HOI Texas Red Unkn IH N/A 0 0 Bar Chart Expression analysis requires that at least two wells contain sample names, targets, and valid Cqs. QC Parameters Data Description Value Use Results Exclude Wells All excluded wells Negative control with a Cq less than 38 True False NTC with a Cq less than 38 True False NRT with a Cq less than 38 True False Positive control with a Cq greater than 30 True False Data Description Value Use Results Exclude Wells All excluded wells Unknown without a Cq N/A True Cy5:Al, A2, Bl, C1,D1, El. FAM:A1, A2, CI, Fl. HEX:B2. Texas Red:Al, A2, Bl, C1,D1, El, Fl, Gl, HI. False Standard without a Cq N/A True False Efficiency greater than 110.0 True Efficiency less than 90.0 True Std Curve RA2 less than 0.980 True Replicate group Cq Std Dev greater than 0.20 True False