design 1 2 3 4 5 6 7 8 9 10 11 12 A medium blank C5 blank C4 blank C3 blank C2 blank C1 medium blank PC1 blank PC2 blank PC3 blank PC4 medium C diuron B medium "1,23 ug/L" "3,7 ug/L" "11,1 ug/L" "33,3 ug/L" 100 ug/L medium 10 5 2.5 1.25 medium PC dichroman draselný C medium "1,23 ug/L" "3,7 ug/L" "11,1 ug/L" "33,3 ug/L" 100 ug/L medium 10 5 2.5 1.25 medium D medium "1,23 ug/L" "3,7 ug/L" "11,1 ug/L" "33,3 ug/L" 100 ug/L medium 10 5 2.5 1.25 medium E medium SC SC SC SC SC medium SC SC SC SC medium F medium "1,23 ug/L" "3,7 ug/L" "11,1 ug/L" "33,3 ug/L" 100 ug/L medium 10 5 2.5 1.25 medium G medium "1,23 ug/L" "3,7 ug/L" "11,1 ug/L" "33,3 ug/L" 100 ug/L medium 10 5 2.5 1.25 medium H medium blank C5 blank C4 blank C3 blank C2 blank C1 medium blank PC1 blank PC2 blank PC3 blank PC4 medium BLANK SUBSTRACTIONS GROWTH RATE CALCULATIONS GROWTH RATE INHIBITION % 0h 1 2 3 4 5 6 7 8 9 10 11 12 0h 1 2 3 4 5 6 7 8 9 10 11 12 A 0.029 0.036 0.03 0.03 0.029 0.028 0.03 0.026 0.026 0.026 0.027 0.026 A avg control 0.561 0.092 16.423 B 0.046 0.043 0.037 0.037 0.037 0.036 0.035 0.039 0.034 0.034 0.027 B 0.012 0.013 0.006 0.008 0.008 0.007 0.008 0.012 0.000 0.007 0-72h 0.592 0.503 0.553 -0.170 0.091 0.666 0.112 0.063 0.675 0-72h -5.579 10.381 1.487 130.347 83.845 -18.782 80.011 88.757 -20.266 C 0.029 0.037 0.037 0.035 0.037 0.038 0.046 0.06 0.035 0.034 0.028 C 0.003 0.007 0.006 0.008 0.009 0.019 0.033 0.009 0.007 0.999 0.536 0.000 0.121 0.472 -0.189 -0.298 0.453 0.635 -77.972 4.385 100.000 100.000 78.440 15.920 133.649 153.101 19.316 -13.128 D 0.032 0.038 0.036 0.035 0.037 0.036 0.037 0.035 0.036 0.034 0.033 0.027 D 0.004 0.006 0.004 0.008 0.007 0.008 0.008 0.009 0.008 0.006 0.936 0.697 0.666 0.000 0.135 0.566 -0.048 0.082 0.472 0.707 -66.801 -24.208 -18.704 100.000 75.912 -0.916 108.501 85.438 15.814 -25.962 E 0.031 0.037 0.037 0.037 0.035 0.036 0.026 0.038 0.035 0.038 0.038 0.028 E 0.009 0.009 0.009 0.007 0.008 0.007 0.010 0.007 0.010 0.010 0.602 0.572 0.559 0.652 0.609 0.552 0.518 0.281 0.563 0.612 -7.363 -1.921 0.403 -16.177 -8.622 1.705 7.621 49.952 -0.293 -9.128 F 0.029 0.037 0.036 0.036 0.035 0.036 0.035 0.044 0.035 0.035 0.043 0.029 F 0.003 0.006 0.005 0.006 0.007 0.006 0.017 0.008 0.009 0.016 0.924 0.666 0.568 0.103 0.165 0.565 -0.311 0.149 0.412 0.657 -64.716 -18.613 -1.277 81.574 70.507 -0.784 155.343 73.487 26.526 -17.174 G 0.029 0.038 0.034 0.037 0.039 0.037 0.035 0.054 0.035 0.036 0.035 0.028 G 0.004 0.004 0.006 0.010 0.008 0.006 0.027 0.008 0.010 0.008 0.886 0.862 0.519 -0.126 0.330 0.626 -0.225 0.220 0.427 0.611 -57.983 -53.672 7.433 122.545 41.268 -11.615 140.068 60.707 23.901 -8.871 H 0.031 0.033 0.031 0.032 0.03 0.03 0.029 0.029 0.028 0.028 H avg 0.867 0.653 0.577 -0.039 0.168 0.579 -0.132 0.043 0.441 0.657 avg -46.736 -13.941 14.890 86.382 56.892 -2.412 104.199 85.240 17.053 -15.755 avg 0.030 0.035 0.031 0.031 0.030 0.029 0.030 0.028 0.027 0.026 0.027 0.028 0.008 avg 0.005 0.007 0.005 0.007 0.008 0.007 0.015 0.014 0.007 0.009 sd 0.159 0.143 0.063 0.110 0.094 0.074 0.166 0.201 0.027 0.037 sd 31.850 23.570 42.612 53.203 35.424 11.921 54.223 36.339 10.538 6.704 0.002 sd 0.004 0.003 0.001 0.002 0.001 0.001 0.008 0.011 0.004 0.004 cv % 18.328 21.931 10.928 -283.827 55.858 12.724 -125.936 463.983 6.060 5.602 20.884 cv % 81.576 51.053 18.237 23.568 7.207 19.460 52.573 76.765 56.758 46.438 24h 1 2 3 4 5 6 7 8 9 10 11 12 24h 1 2 3 4 5 6 7 8 9 10 11 12 A 0.03 0.039 0.032 0.038 0.032 0.028 0.032 0.029 0.031 0.035 0.03 0.03 A avg control 0.555 0.171 30.782 B 0.051 0.051 0.042 0.039 0.038 0.042 0.037 0.038 0.041 0.043 0.033 B 0.014 0.018 0.007 0.008 0.008 0.011 0.008 0.005 0.006 0.013 0-48h 0.754 0.385 0.325 -0.381 0.059 0.562 0.063 -0.144 0.693 0-48h -35.908 30.636 41.401 168.647 89.391 -1.233 88.726 125.912 -24.865 C 0.03 0.044 0.041 0.039 0.039 0.043 0.041 0.039 0.041 0.04 0.031 C 0.007 0.008 0.008 0.009 0.012 0.012 0.006 0.006 0.010 1.014 0.586 0.000 0.059 0.284 -0.283 -0.775 0.053 0.549 -82.677 -5.628 100.000 100.000 89.391 48.841 151.016 239.664 90.510 1.047 D 0.036 0.047 0.041 0.04 0.042 0.038 0.044 0.037 0.037 0.041 0.041 0.031 D 0.010 0.008 0.005 0.011 0.008 0.013 0.008 0.004 0.006 0.011 0.819 0.564 0.483 -0.155 0.126 0.409 -0.072 -0.405 0.000 0.650 -47.501 -1.642 13.074 127.936 77.364 26.294 112.889 173.041 100.000 -17.028 E 0.033 0.044 0.042 0.044 0.045 0.044 0.038 0.044 0.038 0.047 0.048 0.03 E 0.013 0.011 0.013 0.014 0.013 0.007 0.013 0.007 0.016 0.017 0.451 0.600 0.774 0.720 0.758 0.632 0.651 0.158 0.565 0.664 18.678 -8.082 -39.518 -29.694 -36.513 -13.822 -17.225 71.455 -1.801 -19.557 F 0.03 0.044 0.048 0.041 0.043 0.039 0.042 0.042 0.039 0.041 0.058 0.032 F 0.007 0.015 0.006 0.012 0.009 0.011 0.013 0.006 0.006 0.028 1.040 0.777 0.371 0.218 0.178 0.534 -0.394 -0.067 0.144 0.617 -87.297 -39.911 33.173 60.791 67.874 3.819 171.017 112.027 74.088 -11.215 G 0.031 0.045 0.041 0.044 0.042 0.037 0.041 0.039 0.038 0.042 0.043 0.032 G 0.008 0.008 0.009 0.011 0.007 0.010 0.010 0.005 0.007 0.013 0.983 0.919 0.405 -0.118 0.112 0.564 -0.417 -0.235 0.048 0.483 -77.090 -65.575 26.959 121.292 79.901 -1.642 175.191 142.334 91.415 13.074 H 0.037 0.035 0.034 0.032 0.03 0.032 0.031 0.03 0.035 0.032 H avg 0.922 0.646 0.396 -0.087 0.107 0.471 -0.221 -0.325 0.061 0.598 avg -51.966 -15.034 29.182 91.495 61.235 10.376 113.602 144.072 70.842 -9.757 avg 0.032 0.037 0.033 0.035 0.031 0.030 0.032 0.030 0.033 0.035 0.030 0.031 0.012 avg 0.009 0.011 0.007 0.010 0.008 0.011 0.010 0.005 0.006 0.015 sd 0.127 0.206 0.066 0.219 0.050 0.122 0.209 0.281 0.060 0.083 sd 40.227 33.392 45.088 69.094 48.565 22.981 72.422 57.636 41.677 14.264 0.003 sd 0.003 0.005 0.002 0.002 0.001 0.001 0.002 0.001 0.000 0.007 cv % 13.803 31.900 16.751 -251.163 47.075 25.962 -94.803 -86.526 98.430 13.931 23.978 cv % 32.061 41.885 25.301 18.708 10.203 10.460 23.509 16.090 7.213 49.216 48h 1 2 3 4 5 6 7 8 9 10 11 12 48h 1 2 3 4 5 6 7 8 9 10 11 12 A 0.031 0.04 0.037 0.041 0.041 0.029 0.033 0.029 0.031 0.037 0.03 0.031 A avg control 0.347 0.219 63.294 B 0.089 0.062 0.048 0.039 0.039 0.051 0.038 0.042 0.049 0.058 0.033 B 0.052 0.027 0.012 0.004 0.009 0.020 0.009 0.009 0.012 0.028 0-24h 0.197 0.365 0.154 0.065 0.000 0.480 0.000 -0.875 0.619 0-24h 43.262 -5.175 55.538 81.385 100.000 -38.324 100.000 352.513 -78.551 C 0.03 0.056 0.056 0.041 0.039 0.046 0.04 0.04 0.047 0.051 0.03 C 0.019 0.021 0.006 0.009 0.015 0.011 0.007 0.010 0.021 1.030 0.208 0.375 0.118 0.302 -0.475 -1.705 -0.405 0.357 -196.975 40.110 100.000 -8.074 66.028 12.813 237.127 591.704 216.949 -2.876 D 0.035 0.055 0.052 0.047 0.041 0.039 0.048 0.036 0.037 0.045 0.052 0.032 D 0.018 0.017 0.011 0.006 0.009 0.017 0.007 0.004 0.008 0.022 1.050 0.375 0.223 0.383 0.134 0.511 0.000 -0.811 -0.288 0.606 -202.802 -8.074 35.638 -10.467 61.485 -47.338 100.000 333.898 182.977 -74.829 E 0.033 0.054 0.062 0.075 0.062 0.069 0.054 0.069 0.041 0.063 0.07 0.029 E 0.023 0.031 0.044 0.031 0.038 0.023 0.038 0.010 0.032 0.039 0.298 0.125 0.298 0.614 0.410 0.000 0.196 -0.107 0.409 0.472 14.153 63.899 14.153 -77.043 -18.381 100.000 43.387 130.933 -18.105 -35.998 F 0.03 0.057 0.061 0.047 0.044 0.04 0.047 0.037 0.04 0.049 0.085 0.031 F 0.020 0.026 0.011 0.008 0.010 0.016 0.008 0.007 0.012 0.055 1.030 1.003 0.182 0.780 0.251 0.647 -0.278 -0.288 -0.405 0.560 -196.975 -189.384 47.413 -125.023 27.513 -86.508 180.078 182.977 216.949 -61.411 G 0.03 0.062 0.057 0.05 0.043 0.04 0.048 0.041 0.038 0.048 0.051 0.03 G 0.025 0.022 0.014 0.007 0.010 0.017 0.012 0.005 0.011 0.021 0.827 0.827 0.405 0.147 -0.134 0.547 -1.026 -0.470 -0.357 0.486 -138.441 -138.441 -16.949 57.715 138.515 -57.641 395.889 235.564 202.876 -40.036 H 0.037 0.034 0.033 0.032 0.03 0.031 0.029 0.03 0.035 0.031 H avg 0.826 0.555 0.241 0.350 0.074 0.497 -0.356 -0.830 -0.364 0.525 avg -112.963 -39.511 39.299 -13.584 62.527 -19.500 176.080 304.598 160.329 -48.950 avg 0.032 0.037 0.035 0.037 0.036 0.030 0.031 0.030 0.033 0.037 0.030 0.031 0.026 avg 0.027 0.023 0.012 0.006 0.009 0.017 0.009 0.006 0.011 0.029 sd 0.364 0.341 0.113 0.278 0.146 0.126 0.425 0.546 0.056 0.108 sd 112.607 101.439 39.557 78.215 54.706 66.930 127.470 164.500 100.714 28.535 0.011 sd 0.014 0.004 0.001 0.002 0.001 0.002 0.002 0.002 0.002 0.015 cv % 44.002 61.336 46.864 79.533 197.991 25.293 -119.462 -65.770 -15.317 20.525 40.219 cv % 53.516 17.864 12.298 31.957 5.827 11.005 23.299 30.459 15.786 49.676 72h 1 2 3 4 5 6 7 8 9 10 11 12 72h 1 2 3 4 5 6 7 8 9 10 11 12 GRI diuron ug/L PC ug/L A 0.03 0.04 0.044 0.042 0.047 0.027 0.031 0.028 0.027 0.028 0.028 0.03 A avg control 0.573 0.242 42.236 c 1.23 3.70 11.10 33.30 100.00 1.25 2.5 5 10 B 0.105 0.095 0.067 0.043 0.039 0.078 0.039 0.044 0.06 0.081 0.03 B 0.068 0.057 0.032 0.005 0.011 0.048 0.011 0.015 0.032 0.053 48-72h 0.268 0.738 1.008 0.251 0.154 0.875 0.211 0.477 0.981 0.638 log c 0.09 0.57 1.05 1.52 2.00 0.10 0.40 0.70 1.00 C 0.031 0.087 0.071 0.044 0.04 0.065 0.039 0.043 0.063 0.075 0.029 C 0.050 0.033 0.006 0.012 0.035 0.011 0.014 0.035 0.047 0.968 0.437 0.000 0.245 0.847 0.000 0.657 1.253 0.806 0h AVG D 0.035 0.095 0.083 0.065 0.046 0.039 0.071 0.035 0.041 0.061 0.078 0.03 D 0.058 0.045 0.030 0.008 0.011 0.041 0.007 0.012 0.033 0.050 1.170 0.962 1.033 0.310 0.154 0.880 0.000 1.056 1.417 0.821 SD E 0.031 0.087 0.082 0.08 0.082 0.082 0.064 0.079 0.047 0.086 0.095 0.029 E 0.057 0.052 0.050 0.052 0.052 0.034 0.049 0.017 0.056 0.065 0.904 0.516 0.127 0.516 0.313 0.391 0.253 0.526 0.558 0.510 24h AVG -112.96 -39.51 39.30 -13.58 62.53 176.08 304.60 160.33 -48.95 F 0.03 0.077 0.079 0.063 0.046 0.04 0.06 0.035 0.042 0.059 0.143 0.031 F 0.040 0.041 0.028 0.008 0.012 0.030 0.007 0.013 0.031 0.115 0.693 0.443 0.963 -0.125 0.140 0.629 -0.143 0.580 0.949 0.738 SD 112.61 101.44 39.56 78.22 54.71 127.47 164.50 100.71 28.53 G 0.03 0.087 0.085 0.064 0.045 0.05 0.066 0.042 0.045 0.064 0.078 0.03 G 0.050 0.047 0.029 0.007 0.022 0.036 0.014 0.016 0.036 0.050 0.693 0.748 0.747 -0.143 0.765 0.750 0.160 1.131 1.186 0.868 48h AVG -51.97 -15.03 29.18 91.50 61.23 113.60 144.07 70.84 -9.76 H 0.037 0.034 0.033 0.029 0.03 0.03 0.029 0.029 0.032 0.03 H avg 0.758 0.666 0.938 0.059 0.292 0.796 0.046 0.780 1.157 0.774 SD 40.23 33.39 45.09 69.09 48.57 72.42 57.64 41.68 14.26 avg 0.032 0.037 0.039 0.036 0.039 0.029 0.030 0.029 0.030 0.028 0.028 0.030 0.045 avg 0.053 0.044 0.029 0.006 0.013 0.038 0.010 0.014 0.033 0.063 sd 0.340 0.225 0.130 0.211 0.268 0.107 0.142 0.294 0.195 0.089 72h AVG -46.74 -13.94 14.89 86.38 56.89 104.20 85.24 17.05 -15.75 0.013 sd 0.010 0.009 0.002 0.001 0.005 0.007 0.003 0.002 0.002 0.029 cv % 44.808 33.754 13.890 360.039 91.906 13.490 310.144 37.740 16.841 11.511 SD 31.85 23.57 42.61 53.20 35.42 54.22 36.34 10.54 6.70 27.854 cv % 19.643 19.872 5.839 20.696 36.048 17.945 31.579 11.712 6.209 46.264 For GraphPad prism For GraphPad prism avg control 0.764 0.337 44.181 72h % Growth rate inhibition c log c 1 2 3 4 5 c log c 1 2 3 4 5 Graph Pad avg sd cv% 24-48h 1.312 0.405 0.496 -0.827 0.118 0.644 0.125 0.588 0.693 0.767 "1,23 ug/L" 0.090 -5.58 -77.97 -66.80 -64.72 -57.98 SC 0 -18.78 15.92 -0.92 3.82 -1.64 log(agonist) vs. normalized response -- Variable slope OD 0 0.009 0.001 15.863 0.999 0.965 -0.375 0.000 0.266 -0.091 0.154 0.511 0.742 "3,7 ug/L" 0.568 10.38 4.39 -24.21 -18.61 -53.67 1.25 0.097 80.01 133.65 108.50 155.34 140.07 Best-fit values 1 0.012 0.003 27.716 0.588 0.754 0.742 -0.693 0.118 0.307 -0.143 0.000 0.288 0.693 "11,1 ug/L" 1.045 1.49 100.00 -18.70 -1.28 7.43 2.5 0.398 88.76 153.10 85.44 73.49 60.71 LOGEC50 1.093 2 0.031 0.010 32.297 0.605 1.075 1.251 0.826 1.105 1.264 1.105 0.424 0.721 0.856 "33,3 ug/L" 1.522 130.35 100.00 100.00 81.57 122.54 5 0.699 0.00 19.32 15.81 26.53 23.90 HILLSLOPE 11.93 3 0.049 0.014 27.877 1.050 0.550 0.560 -0.345 0.105 0.421 -0.511 0.154 0.693 0.675 100 ug/L 2.000 83.84 78.44 75.91 70.51 41.27 10 1.000 -20.27 -13.13 -25.96 -17.17 -8.87 EC50 12.40 1.139 1.012 0.405 -0.383 0.357 0.582 0.191 0.000 0.452 0.480 Std. Error avg 1.018 0.737 0.551 -0.524 0.140 0.444 -0.086 0.179 0.527 0.671 Diuron - Growth Rate Inhibition 72h - Raphidocelis subcapitata LOGEC50 ~ 90.62 sd 0.268 0.261 0.142 0.221 0.131 0.166 0.276 0.241 0.172 0.113 EC50 12.40 ug/L HILLSLOPE ~ 22408 cv % 26.359 35.410 25.820 -42.044 93.955 37.357 -322.134 134.502 32.620 16.891 EC20 11.03 ug/L 95% Confidence Intervals LOGEC50 (Very wide) HILLSLOPE (Very wide) EC50 (Very wide) Goodness of Fit Degrees of Freedom 23 R2 "0,6050" Absolute Sum of Squares 41119 Sy.x "42,28" Number of points Analyzed 25