S2004/S2005 Methods for characterization of biomolecular interactions – classical vs. modern

                                            Autumn 2022


Teachers: Josef Houser, Monika Kubíčková, Jan Komárek, Eva Paulenová,
Michaela Wimmerová


Students enrolled in S2004 course and Workshop participants attend only lectures (L). Students
enrolled in both S2004 and S2005 courses need to attend both lectures (L) and practical exercises
(P). In the practical part, students are divided into two groups – P1 and P2 (see separate list).


January 9, 2022 (Monday)

time


program

room

10:00-10:05

                                                 L

Introduction

C04/211

10:05-11:30

                                                 L

Biomolecular interactions (Wimmerová)

C04/211

11:30-12:30


lunch


12:30-14:00

                                                 L

Isothermal titration calorimetry (ITC) (Kubíčková)

C04/211

14:00-14:15


break


14:15-15:15

                                                 L

Spectroscopic methods (Houser)

C04/211

15:15-15:30


break


15:30-16:30

                                                 L

Microscale thermophoresis (MST) (Paulenová)

C04/211


January 10, 2022 (Tuesday)

time


program

room

09:00-10:30

                                                 L

Analytical ultracentrifugation (AUC) (Komárek)

C04/211

10:30-10:45


break


10:45-12:15

                                                 L

Surface-based methods (Houser)

C04/211

12:15-13:15


lunch


13:15-13:45

                                                 L

Interactions at the cell level (Houser)

C04/211

13:45-14:45

                                                 L

Importance of sample preparation (Houser)

C04/211

14:45-15:00


break


15:00-17:00

                                                P1

ITC – hands-on (Kubíčková)

C04/218


                                                P2

AUC – hands-on (Komárek)

C04/217



January 11, 2022 (Wednesday)

time


                                                  program

                                                                                 room

09:00-10:30

                                                           P1

                                                  ITC – data analysis (Kubíčková)

                                                                                 C04/218


           P2

                                                  AUC – data analysis (Komárek)

                                                                                 C04/211

10:30-10:45


                                                  break


10:45-12:00

                                                           P1

                                                  BLI – hands-on (Houser)

                                                                                 C04/218


           P2

                                                  MST – hands-on (Paulenová)

                                                                                 C04/221

12:00-13:00


                                                  lunch


13:00-13:45

                                                           P1

                                                  BLI – data analysis (Houser)

                                                                                 C04/218


           P2

                                                  MST – data analysis (Paulenová)

                                                                                 C04/221

13:45-14:00


                                                  break


14:00-16:00

                                                           P1

                                                  AUC – hands-on (Komárek)

                                                                                 C04/217


           P2

                                                  ITC – hands-on (Kubíčková)

                                                                                 C04/218


January 12, 2022 (Thursday)

time


program

room

09:00-10:30

                                                P1

AUC – data analysis (Komárek)

C04/1S57


                                                P2

ITC – data analysis (Kubíčková)

C04/218

10:30-10:45


break


10:45-12:00

                                                P1

MST – hands-on (Paulenová)

C04/221


                                                P2

BLI – hands-on (Houser)

C04/218

12:00-13:00


lunch


13:00-13:45

                                                P1

MST – data analysis (Paulenová)

C04/221


                                                P2

BLI – data analysis (Houser)

C04/218

13:45-14:00


break


14:00-15:00

                                                 P

Discussion of practical aspects (all teachers)

C04/218


January 13, 2022 (Friday)

time


                                                  program

                                                              room

09:30-11:00

                                                            L

                                                  Written test

                                                              C04/211
                                                              C04/218