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20231212cviceni2blaZ Experiment
Creation Date I 12/12/2023 2:43:20 PM		Last Modified Date	12/12/2023 4:47:45 PM
Operator I LMDM		Owner	LMDM
Start Time | 12/12/2023 3:33:43 PM		End Time	12/12/2023 4:46:11 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	02122014blaZkvantifikace Run Protocol, 1_11_2022_PRAKTIKUM_SAU Run Protocol, gene blaZ absolute quantification SYBER GREEN, 1_11_2022_PRAKTIKUM_SAU Run Protocol	Plate ID	B8681963
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Program Name		stage 1							
Cycles		1	Analysis Mode | None						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name		stage 2							
Cycles		1	Analysis Mode		None				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		0	0	0
Program Name		stage 3							
Cycles		40	Analysis Mode		Quantification				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	None		00:00:30	2.20		0	0	0
	72	Single		00:00:10	4.40		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode | Melting Curves						
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Target	Acquisition	Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
(°C)	Mode	(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
95	None	00:00:05	4.40		0	0	0
65	None	00:01:00	2.20		0	0	0
97	Continuous		0.11	5	0	0	0
Program Name		cooling						
Cycles		1              Analysis Mode		None				
	Target	Acquisition	Hold	Ramp Rate	Acquisitions	Sec Target	Step size	Step Delay
	(°C)	Mode	(hh:mm:ss)	(°C/s)	(per°C)	(°C)	(°C)	(cycles)
	40	None	00:01:00	2.20		0	0	0
Samples
Sample Count 96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	JB blaZ			
A2	JB blaZ			
A3	JB blaZ			
A4	Sample 4			
A5	Sample 5			
A6	Sample 6			
A7	Sample 7			
A8	Sample 8			
A9	Sample 9			
A10	Sample 10			
A11	Sample 11			
A12	Sample 12			
B1	Sample 13			
B2	Sample 14			
B3	Sample 15			
B4	Sample 16			
B5	Sample 17			
B6	Sample 18			
B7	Sample 19			
B8	Sample 20			
B9	Sample 21			
B10	Sample 22			
B11	Sample 23			
B12	Sample 24			
C1	Tr1			
C2	Tr1			
C3	Tr1			
C4	Tr1 10*			
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Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C5	Tr1 10*			
C6	Tr1 10*			
C7	Sample 31			
C8	Sample 32			
C9	Sample 33			
C10	Sample 34			
C11	Sample 35			
C12	Sample 36			
D1	Tr2			
D2	Tr2			
D3	Tr2			
D4	Tr2 10*			
D5	Tr2 10*			
D6	Tr2 10*			
D7	Sample 43			
D8	Sample 44			
D9	Sample 45			
D10	Sample 46			
D11	Sample 47			
D12	Sample 48			
E1	H20			
E2	H20			
E3	H20			
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
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Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
F8	Sample 68			
F9	Sample 69			
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Instrument
Name	29712	Serial Number	29712
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max)
20231212cviceni2blaZ
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Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
Subset Name All Samples
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
□	A1	JB blaZ	Standard			2.43E3	
0	A2	JB blaZ	Standard	17.47	3.09E3	2.43E3	
0	A3	JB blaZ	Standard	17.83	2.37E3	2.43E3	
0	A4	Sample 4	Standard	21.13	2.05E2	2.43E2	
0	A5	Sample 5	Standard	21.82	1.23E2	2.43E2	
0	A6	Sample 6	Standard	20.59	3.06E2	2.43E2	
0	A7	Sample 7	Standard	24.02	2.38E1	2.43E1	
□	A8	Sample 8	Standard			2.43E1	
0	A9	Sample 9	Standard	23.56	3.37E1	2.43E1	
0	A10	Sample 10	Standard	27.55	1.73E0	2.43E0	
0	A11	Sample 11	Standard	27.45	1.86E0	2.43E0	
0	A12	Sample 12	Standard	27.87	1.37E0	2.43E0	
0	B1	Sample 13	Standard	30.24	2.34E-1	2.43E-1	
0	B2	Sample 14	Standard	29.76	3.35E-1	2.43E-1	
0	B3	Sample 15	Standard	29.86	3.11E-1	2.43E-1	
0	B4	Sample 16	Standard	32.87	2.58E-2	2.43E-2	
0	B5	Sample 17	Standard	33.36	1.62E-2	2.43E-2	
0	B6	Sample 18	Standard	32.93	2.43E-2	2.43E-2	
0	B7	Sample 19	Unknown				
0	B8	Sample 20	Unknown				
0	B9	Sample 21	Unknown				
0	B10	Sample 22	Unknown	30.41	2.06E-1		
0	B11	Sample 23	Unknown				
0	B12	Sample 24	Unknown	31.83	6.43E-2		
0	C1	Tr1	Unknown	16.68	5.57E3		E
0	C2	Tr1	Unknown	16.28	7.51 E3		E
0	C3	Tr1	Unknown	16.89	4.78E3		E
0	C4	Tr1 10*	Unknown	19.99	4.78E2		
0	C5	Tr1 10*	Unknown	20.18	4.14E2		
0	C6	Tr1 10*	Unknown	20.17	4.17E2		
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty, ? - Detector Call uncertain
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	C7	Sample 31	Unknown				
0	C8	Sample 32	Unknown				
0	C9	Sample 33	Unknown				
0	C10	Sample 34	Unknown				
0	C11	Sample 35	Unknown				
0	C12	Sample 36	Unknown				
0	D1	Tr2	Unknown	33.30	1.73E-2		E
0	D2	Tr2	Unknown	33.38	1.60E-2		E
0	D3	Tr2	Unknown	34.01	8.73E-3		E
0	D4	Tr2 10*	Unknown	35.00	3.20E-3		>, E
0	D5	Tr2 10*	Unknown	35.00	3.20E-3		>, E
0	D6	Tr2 10*	Unknown	35.00	3.20E-3		>, E
0	D7	Sample 43	Unknown				
0	D8	Sample 44	Unknown				
0	D9	Sample 45	Unknown				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	H20	Negative Control	35.00	3.20E-3		>
0	E2	H20	Negative Control	35.00	3.20E-3		>
0	E3	H20	Negative Control	35.00	3.20E-3		>
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown	32.00	5.55E-2		
0	E12	Sample 60	Unknown	31.67	7.41 E-2		?
0	F1	Sample 61	Unknown	23.02	5.04E1		?
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown				
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown	33.30	1.71 E-2		E
0	F7	Sample 67	Unknown				
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty, ? - Detector Call uncertain
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Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	F8	Sample 68	Unknown	22.70	6.36E1		
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown	32.39	3.95E-2		
0	F11	Sample 71	Unknown				
0	F12	Sample 72	Unknown				
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown				
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown				
0	H7	Sample 91	Unknown				
0	H8	Sample 92	Unknown				
0	H9	Sample 93	Unknown				
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown	25.13	1.05E1		?
0	H12	Sample 96	Unknown				
E - Extrapolated concentration in standard curve, > - Late Cp call (last five cycles) has higher uncertainty, ? - Detector Call uncertain
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Amplification Curves
42.21 CH
5~ 10 15 20 25 30
Cycles
Standard Curve
— Std. curve * Samples
Error: 0.0355 Efficiency: 2.102 Slope: -3.099 Ylntercept: 28.29 Link: 0.311
n i i i r
-10 12 3
Log Concentration
Analysis Notes
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