Lectures

1. Introduction to fluoresce applications
2. Colorful definitions of absorbption, fluorescence a fosforescence
3. Instrumentation for the detection of absorption and fluorescence
4. Steady state fluorescence
5. Absoroption spectroscopy in biomolecule analysis
6. Time-resolved fluorescence
7. Anizotropy of fluorescence: monitoring of biomolecule binding
8. Fluorescence resonance energy transfer
9. Intrinsic fluorescence of proteins, quenching
10. Extrinsic fluorescence – labels, probes, indicators
11. Fluorescence labeling of DNA and proteins
12. Fluorescence microscopy
13. Analytical appliacations of fluorescence
14. Examples of fluorescence appliacations in research and biological practice