Molecular Cell Biology
EIGHTH EDITION
© 2016 W. H. Freeman and Company
Lodish • Berk • Kaiser • Krieger • Bretscher •
Ploegh • Amon • Martin
Evolution of life
Evolution of life
All living organisms descended from a common
ancestral cell.
Molecular cell biology: in perspective
The Cell as matter
Morphological variety of cells
Eubacteria Archaebacteria Blood cells
Alga colony Neuron Plant cells
Emerging view of a cell
Bacterial cell
Mycoplasma mycoides illustration by David Goodsell
Inside a bacterial cell
Mycoplasma mycoides illustration by David Goodsell
Key for “Mycoplasma mycoides”
Protein synthesis (labels in black)
1.DNA
2.DNA polymerase
3.single-stranded-DNA binding protein (protects single-stranded portions during replication)
4.RNA polymerase
5.messenger RNA
6.ribosome
7.transfer RNA(in pink) and elongation factor Tu (in blue)
8.elongation factor Tu and Ts
9.elongation factor G
10.aminoacyl-tRNA synthetases
11.topoisomerases
12.Rec system for DNA repair: a) RecA, b) RecBC
13.chaperonin GroEL (helps folding of new proteins)
14.ClpA (destroys old proteins)
Enzymes for energy production (labels in red)glycolytic enymes
1.pyruvate dehydrogenase complex
2.
Membrane proteins (labels in blue)ATP synthase
3.secretory proteins
4.sodium pump
5.zinc transporter
6.magnesium transporter
7.ABC transporter (different ABC transporters transport different types of molecules-ABC is
short for "ATP-binding cassette")
8.magnesium transporter
9.lipoglycan (long carbohydrate chains connected to lipid in the membrane)
3D model of a bacterial cell
Eukaryotic cell
Clicker Question 1-1
All of the following are macromolecules EXCEPT:
A. polysaccharides.
B. proteins.
C. nucleic acids.
D. sugars.
E. All of the above are macromolecules.
Clicker Question 1-1
All of the following are macromolecules EXCEPT:
A. polysaccharides.
B. proteins.
C. nucleic acids.
D. sugars.
E. All of the above are macromolecules.
Sugars are the monomers used to form polysaccharides.
Clicker Question 1-2
DNA is converted into RNA in a process called:
A. transcription.
B. translation.
C. complementary base pairing (G=C, A=T).
D. genetic coding.
E. catalyzation
Clicker Question 1-2
DNA is converted into RNA in a process called:
A. transcription.
B. translation.
C. complementary base pairing (G=C, A=T).
D. genetic coding.
E. catalyzation
Clicker Question 1-3
Phospholipid bilayers contain:
A. cholesterol.
B. transmembrane proteins.
C. hydrophobic fatty acyl chains.
D. hydrophilic head groups.
E. All of the above
Clicker Question 1-3
Phospholipid bilayers contain:
A. cholesterol.
B. transmembrane proteins.
C. hydrophobic fatty acyl chains.
D. hydrophilic head groups.
E. All of the above
Clicker Question 1-4
_____ is the most common molecule used by cells to capture, store, and transfer
energy.
A. ATP
B. ADP
C. L-glucose
D. D-glucose
E. Water
Clicker Question 1-4
_____ is the most common molecule used by cells to capture, store, and transfer
energy.
A. ATP
B. ADP
C. L-glucose
D. D-glucose
E. Water
Clicker Question 1-5
The DNA double helix is stabilized by weak hydrogen bonds between:
A. G and C bases only.
B. A and T bases only.
C. A and U bases only.
D. A and T bases and between G and C bases.
E. A and U bases and between G and C bases.
Clicker Question 1-5
The DNA double helix is stabilized by weak hydrogen bonds between:
A. G and C bases only.
B. A and T bases only.
C. A and U bases only.
D. A and T bases and between G and C bases.
E. A and U bases and between G and C bases.
Clicker Question 1-6
TRUE or FALSE: Water is a component of the phospholipid bilayer.
A. True
B. False
Clicker Question 1-6
TRUE or FALSE: Water is a component of the phospholipid bilayer.
A. True
B. False
Clicker Question 1-2
In the diagram below, which arrow indicates the process of translation?
A. red
B. yellow
C. green
D. blue
E. purple
DNA RNA protein
Clicker Question 1-2
In the diagram below, which arrow indicates the process of translation?
A. red
B. yellow
C. green
D. blue
E. purple
DNA RNA protein