1) Molecular biology and its position in biological sciences, milestones, personalities, central dogma of molecular biology
2) Information macromolecules, their structure, function and interaction in gene expression, genetic code.
3) Structure of prokaryotic genome, replication and gene expression in prokaryotes.
4) Structure of eukaryotic genome, replication and gene expression in eukaryotes.
5) Regulation of gene expression, molecular signalling.
6) Mutagenesis, spontaneous and induced mutation and reversion.
7) Molecular basis of recombination, recombinantion in genetics. Reparation and excision mechanisms.
8) RNA interference
9) Regulations of Cell cycle
10) Apoptosis
11) Transposons, mechanisms of transposition, retroelements, retroviruses.
12) Molecular basis of cancerogenesis, protooncogenes and oncogenes, suppressor genes, oncoviruses.
13) Molecular biology of viruses, replication, expression of bacterial and animal virus's genes.
Exam details:
1) Molecular biology and its position in biological sciences, milestones,
personalities, central dogma of molecular biology
- terms- marks on slides -
TEST
2) Information macromolecules, their structure, function and interaction in
gene expression, genetic code.
- central dogma
of MB, coding of basis (WC, H)
3) Structure of prokaryotic genome, replication and gene expression in
prokaryotes.
·
description of
replication, transcription, translation in procaryotic cells
·
Lac operon
·
description of
replication, transcription, translation in procaryotic cells
5) Regulation of gene expression, molecular signalling.
- transcription factors, chromatin, promoters,
6) Mutagenesis, spontaneous and induced mutation and reversion.
- types of mutations, mutagens (slide 14, p53, BRCA1/2)
7) Molecular basis of recombination, recombination in
genetics. Reparation and excision mechanisms.
- types of reparations, recombination
8) RNA interference
-siRNA, shRNA, CRISPR/CAS
9) Regulations of Cell cycle
-cell cycle phasis, proteins CDK and inhibitors, cyclins (slide 37-43)
10) Apoptosis
-types of cell death, proteins involve in
11) Transposons, mechanisms of transposition, retroelements, retroviruses.
- basic terms
12) Molecular basis of cancerogenesis, protooncogenes and oncogenes,
suppressor genes, oncoviruses.
- Six acquired characteristics of a malignant tumor, hallmarks of cancer (slide 25, marked test)
13) Molecular biology of viruses, replication, expression of bacterial and
animal virus's genes.
- viral genomes (slide 29,31)