LF:BLLM051x Medical Microbiology - pract. - Course Information

BLLM051x Medical Microbiology - practice

Extent and Intensity

0/0/0. 5 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Ondřej Zahradníček (seminar tutor)
Michaela Gregorovičová (assistant)
Iva Holešovská (assistant)

Guaranteed by

prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine

Timetable of Seminar Groups

BLLM051x/1: Tue 17. 10. 7:00–12:50 MiÚ N04308, Wed 18. 10. 7:00–12:50 MiÚ N04308, Thu 19. 10. 7:00–12:50 MiÚ N04308, Fri 20. 10. 7:00–12:50 MiÚ N04308, Tue 24. 10. 7:00–12:50 MiÚ N04308, Wed 25. 10. 7:00–12:50 MiÚ N04308, Thu 26. 10. 7:00–12:50 MiÚ N04308, Fri 27. 10. 7:00–12:50 MiÚ N04308, O. Zahradníček
BLLM051x/2: Tue 31. 10. 7:00–12:50 MiÚ N04308, Wed 1. 11. 7:00–12:50 MiÚ N04308, Thu 2. 11. 7:00–12:50 MiÚ N04308, Fri 3. 11. 7:00–12:50 MiÚ N04308, Tue 7. 11. 7:00–12:50 MiÚ N04308, Wed 8. 11. 7:00–12:50 MiÚ N04308, Thu 9. 11. 7:00–12:50 MiÚ N04308, Fri 10. 11. 7:00–12:50 MiÚ N04308, O. Zahradníček
BLLM051x/3: Tue 12. 12. 7:00–12:50 MiÚ N04308, Wed 13. 12. 7:00–12:50 MiÚ N04308, Thu 14. 12. 7:00–12:50 MiÚ N04308, Fri 15. 12. 7:00–12:50 MiÚ N04308, Tue 19. 12. 7:00–12:50 MiÚ N04308, Wed 20. 12. 7:00–12:50 MiÚ N04308, Thu 21. 12. 7:00–12:50 MiÚ N04308, Fri 22. 12. 7:00–12:50 MiÚ N04308, O. Zahradníček
BLLM051x/4: Tue 28. 11. 7:00–12:50 MiÚ N04308, Wed 29. 11. 7:00–12:50 MiÚ N04308, Thu 30. 11. 7:00–12:50 MiÚ N04308, Fri 1. 12. 7:00–12:50 MiÚ N04308, Tue 5. 12. 7:00–12:50 MiÚ N04308, Wed 6. 12. 7:00–12:50 MiÚ N04308, Thu 7. 12. 7:00–12:50 MiÚ N04308, Fri 8. 12. 7:00–12:50 MiÚ N04308, O. Zahradníček
BLLM051x/5: Tue 3. 10. 7:00–12:50 MiÚ N04308, Wed 4. 10. 7:00–12:50 MiÚ N04308, Thu 5. 10. 7:00–12:50 MiÚ N04308, Fri 6. 10. 7:00–12:50 MiÚ N04308, Tue 10. 10. 7:00–12:50 MiÚ N04308, Wed 11. 10. 7:00–12:50 MiÚ N04308, Thu 12. 10. 7:00–12:50 MiÚ N04308, Fri 13. 10. 7:00–12:50 MiÚ N04308, O. Zahradníček
BLLM051x/6: Tue 19. 9. 7:00–12:50 MiÚ N04308, Wed 20. 9. 7:00–12:50 MiÚ N04308, Thu 21. 9. 7:00–12:50 MiÚ N04308, Fri 22. 9. 7:00–12:50 MiÚ N04308, Tue 26. 9. 7:00–12:50 MiÚ N04308, Wed 27. 9. 7:00–12:50 MiÚ N04308, Fri 29. 9. 7:00–12:50 MiÚ N04308, O. Zahradníček

Prerequisites

BLLM0422c Medical Microbiology II - p.
The students are supposed to have completed subjects BLLM0321 and BLLM0322, including examination, if possible.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Laboratory Technologist (programme LF, B-LABD)
• Laboratory Assistant (programme LF, B-SZ)

Course objectives

Specialist practice complements the practical part of education in Clinical Biochemistry, Clinical Hematology, Immunohematology and Transfusion medicine, Clinical Microbiology, Clinical Immunology, Clinical Genetics and Pathologic Anatomy – Histopathologic determination methods. Students become familiar with the organization of workflow processes and basic documentation of laboratory worksites and will perform select tasks and activities. Students will mostly come to contact with following topics: Material admission and its division into corresponding laboratories; Basics of media preparing for bacterial culture; Specimen processing for bacteriological examination (urine, throat swab, vaginal swab, sputum, pus, blood culture); Assessment of antibiotic susceptibility using diffusion disc test and microdillution method; Basic serological methods (Paul-Bunnell in micro-modifiactions OCH and AKE, complemetfixing test, ASO, latex-fixation test); Use of ELISA and PCR methods;

Learning outcomes

At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting

Syllabus

• 1. Admission of material, its distribution into individual laboratories; expedition of results to individual clients
• 2. Preparing culture media for bacteriological cultivation
• 3. Bacteriology I (processing of individual specimens: throat/nasal swab, sputum, urine, pus, blood culture and other specimens)
• 4. Bacteriology II (identification methods in bacteriology, antibiotic susceptibility testing methods – diffusion disc test, microdillution test)
• 5. Gastrointestinal specimens processing and evaluation, basics of parasitology of gastrointetinal tract
• 6. Laboratory of STI (processing of various specimens for bacteriology, mycology and diagnostics of Trichomonas, including dg. of gonorrhoea)
• 7. Serology: clasical serological methods, modern serological methods
• 8. Virology: clasical and modern methods used in serological virology, direct diagnostics of viruses; basics of PCR in microbiology

Literature

• VOTAVA, Miroslav, Vlastimil OBDRŽÁLEK, Petr ONDROVČÍK, Filip RŮŽIČKA, Ondřej ZAHRADNÍČEK and Vladana WOZNICOVÁ. Lékařská mikrobiologie II. Přehled vyšetřovacích metod v lékařské mikrobiologii (Medical Microbiology II. Survey of Diagnostic Methods in Medical Microbiology). 1st ed. Brno: Masarykova univerzita. Lékařská fakulta, 2000, 309 pp. ISBN 80-210-2272-8. info
• VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology. 2nd revised ed.). 2. přepracované vydání. Brno: NEPTUN, 2005, 351 pp. ISBN 80-86850-00-5. info
• VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1st ed. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003, 495 pp. ISBN 80-902896-6-5. info
• SEDLÁČEK, Ivo. Taxonomie prokaryot (Taxonomy of prokaryotes). 1st ed. Brno: Masarykova univerzita, 2007, 270 pp. 55-969C-2006 02/58 12Př. ISBN 80-210-4207-9. info
• 1 = základní/basic, 2, 3, 4 doplňkové/supplementary.

Teaching methods

practical work in laboratories of Department of microbiology

Assessment methods

credit, condition for credit is to absolve the practice

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
Information on the extent and intensity of the course: 48.

• Enrolment Statistics (autumn 2023, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2023/BLLM051x