Bi6400 Methods of molecular biology

Faculty of Science
Spring 2025
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Ing. Sylva Koudelková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2024
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Ing. Sylva Koudelková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2023
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Ing. Sylva Koudelková, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2022
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2021
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Tue 10:00–12:50 online_B5
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2020
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Enzymes in molecular biology.
  • 5. Polymerase chain reaction.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. Characterization and using of basic types of vectors.
  • 8. Cloning strategies, DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells.
  • 9. Principles of molecular diagnostics.
  • 10. Flow cytometry.
  • 11. In vitro transcription and translation.
  • 12. Protein analyses: electrophoresis, mass spectrometry, sequencing.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein-protein and protein-DNA interactions.
  • 15 DNA methylation, postranslational modifications of proteins.
  • 16 Genome editing, RNA interference.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2019
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
spring 2018
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Learning outcomes
At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2017
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2016
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2015
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Lucia Knopfová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 60 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2014
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and manage to choose the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labeling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry.
  • 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2013
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–12:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4010 Essential molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2012
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 8:00–10:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2011
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 8:00–10:50 B11/306
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2010
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Thu 17:00–19:50 A,01026
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2009
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Fri 8:00–10:50 BR3
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Assessment methods
Examination consists of two parts: written test and oral.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2008
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 9:00–11:50 BR3
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2007
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 10:00–12:50 BR3
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2006
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Thu 9:00–11:50 BR1
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2005
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Fri 8:00–10:50 B1,01004
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
  • Alberts, Bray, Johnson, Lewis, Raff, Roberts, Walter. Základy buněčné biologie. Espero Publishing. 1998.
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/student/6400.htm
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2004
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
  • Alberts, Bray, Johnson, Lewis, Raff, Roberts, Walter. Základy buněčné biologie. Espero Publishing. 1998.
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/student/6400.htm
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2003
Extent and Intensity
3/0/0. 3 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
  • Alberts, Bray, Johnson, Lewis, Raff, Roberts, Walter. Základy buněčné biologie. Espero Publishing. 1998.
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/student/6400.htm
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods a make reasoned decisions about choosing the right metdodical approaches to address specific biological phenomena.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 2. Electrophoretic and microscopic analyses of nucleic acids.
  • 3. Hybridisation of nucleic acids: probe preparation, labelling.
  • 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 5. Sequence analyses of nucleic acids, genome sequencing.
  • 6. Characterization and using of basic types of vectors.
  • 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays.
  • 8. Gene libraries.
  • 9. Flow cytometry. 10. In vitro transcription and translation.
  • 11. Polymerase chain reaction.
  • 12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation.
  • 13. Preparation and use of monoclonal and polyclonal antibodies.
  • 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination is based on written test composed of 50 questions. Correct answering of at least 50% of them is required to pass the test.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika je možné zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6400 Methods of molecular biology

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
SOUHLAS || Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Structure and function of nucleic acids and proteins.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The course is focused on basic techiques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who intend to work in laboratories of basic or applied research. Understanding of principle and practical use of methods is emphasized.
Syllabus
  • 1. Nucleic acids: manipulation, centrifugation, modifications in vitro. 2. Electrophoretic and microscopic analyses of nucleic acids. 3. Hybridisation of nucleic acids: probe preparation, labelling. 4. Restriction analysis of nucleic acids, restriction map construction, genome mapping. 5. Sequence analyses of nucleic acids, genome sequencing. 6. Characterization and using of basic types of vectors. 7. Cloning strategies: DNA transfer to prokaryotic and eukaryotic cells, determination of products of cloned genes in host cells: protein electrophoresis, western blotting, luciferase and ß-galactosidase assays. 8. Gene libraries. 9. Flow cytometry. 10. In vitro transcription and translation. 11. Polymerase chain reaction.12. Protein analyses: electrophoresis in one and two dimensions, immunoprecipitation, focusation. 13. Preparation and use of monoclonal and polyclonal antibodies. 14. Analysis of protein - DNA interactions: retardation analysis, DNA footprinting.
Literature
Assessment methods (in Czech)
Zkouška se provádí formou písemného testu, po kterém následuje krátký ústní pohovor.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Pro obor Biofyzika zakončení kolokviem
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: V případě, že student nesplňuje vyžadované prerekvizity pro zápis, je zápis možný s tím, že ukončení tohoto předmětu je podmíněno úspěšným absolvováním předmětu Molekulární biologie (jeden z kódů v prerekvizitách).
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)