Bi6721 Special Methods of Microorganisms Analysis

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • Persing D.H. a kol. Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition. 2016, 835 p. ISBN: 978-1-555-81908-8
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • Brown T.A. Gene Cloning and DNA Analysis: An Introduction, 8th Edition. 2020, Wiley-Blackwell, 432 p. ISBN: 978-1-119-64078-3
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

Bi6721 Special Methods of Microorganisms Analysis

Faculty of Science
Spring 2024
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • Persing D.H. a kol. Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition. 2016, 835 p. ISBN: 978-1-555-81908-8
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • Brown T.A. Gene Cloning and DNA Analysis: An Introduction, 8th Edition. 2020, Wiley-Blackwell, 432 p. ISBN: 978-1-119-64078-3
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • Persing D.H. a kol. Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition. 2016, 835 p. ISBN: 978-1-555-81908-8
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • Brown T.A. Gene Cloning and DNA Analysis: An Introduction, 8th Edition. 2020, Wiley-Blackwell, 432 p. ISBN: 978-1-119-64078-3
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • Persing D.H. a kol. Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition. 2016, 835 p. ISBN: 978-1-555-81908-8
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • Brown T.A. Gene Cloning and DNA Analysis: An Introduction, 8th Edition. 2020, Wiley-Blackwell, 432 p. ISBN: 978-1-119-64078-3
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martin Benešík, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Tue 12:00–13:50 online_B1
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected methods of molecular biology, biochemistry, and biophysics for analysis of microorganisms, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes, genomes, and proteins 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins and metabolites 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2020
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students who are interested in new methods of analysis of microorganisms. The prerequisite is the passed exam of the course Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the principles of selected molecular biology and chemotaxonomic methods, with the procedure for selection of a suitable method for solving a real problem, differences in procedures used for bacteria and yeasts and interpreting the results of analyzes. Students will receive specific information about the following processes: 1)Isolation of nucleic acids (NA) from microorganisms and analysis of NA using chromatographic and electrophoresis methods 2)Application of enzymes in microbial genes analysis 3)Sequence analysis of microbial genes 4)Application of hybridisation and polymerase chain reaction in microbiology 5)Methods of study of microbial proteins 6)Application of recombinant NA for the study of microorganisms
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology, biochemistry and biophysics methods used in microbiology, propose the procedure for solving a real problem, realize differences between methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Methods for preparation of recombinant DNA and RNA molecules and their use for the study of microorganisms.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of Powerpoint presentations. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answer 15 questions. The overall result of the exam is evaluated by the examiner. Test if followed by a brief discussion with the examiner (oral part of the exam).
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students will be acquainted with the principles of selected molecular biological and chemotaxonomic methods and the suitability of their use for various types of microorganisms.
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
  • Bioinformatic tools in analysis of microorganisms.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of PowerPoint presentations. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
spring 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), Mgr. Iva Buriánková, Ph.D. (deputy)
Guaranteed by
Mgr. Iva Buriánková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Iva Buriánková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 13:00–14:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students will be acquainted with the principles of selected molecular biological and chemotaxonomic methods and the suitability of their use for various types of microorganisms.
Learning outcomes
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Mon 15:00–16:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 15:00–16:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 E25/209
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, to realize differences of methods used for bacteria and yeasts; interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Isolation of DNA and mtDNA from yeasts.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principles of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer), prof. RNDr. Zdeněk Hubálek, DrSc. (deputy)
Mgr. Jana Čmielová, Ph.D. (assistant)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zdeněk Hubálek, DrSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 14:00–15:50 Bpt,01013
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • ROLFS, A. PCR :clinical diagnostics and research. Berlin: Springer-Verlag, 1992, xix, 271 s. ISBN 3-540-55440-8. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2011
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 10:00–11:50 Bpt,01013
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2010
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 10:00–11:50 Bpt,01013
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Mon 12:00–13:50 Bpt,01013
Prerequisites
The course is for student sof mikrobiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the exam from Molecular biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Fragmentation of DNA molecules using restriction enzymes. Methods used in analysis of nucleic acids (electromigration methods, chromatographic methods, spectrophotometric methods, sequencing). Hybridisation of nucleic acids. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites. Chemotaxonomy and bacterial cell components estimation. Principles of quantitative analysis. Techniques which use immunomagnetic and affinity based separations. The course is supplemented with practical course Bi6721c.
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems.The course is followed up by a practical course.
Assessment methods
Oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 12:00–13:50 Bpt,01013
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 15:00–16:50 Bpt,01013
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 10:00–11:50 Bpt,01013
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer), doc. RNDr. Alena Španová, CSc. (deputy)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Timetable
Tue 13:00–14:50 Bpt,01013
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
doc. RNDr. Alena Španová, CSc. (seminar tutor)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2012

The course is not taught in Spring 2012

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites
The course is destined for students of microbiology and other students which are interested in new methods of analysis of microorganisms. The prerequisit is the examination in the course from Molecular Biology (Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to: understand and explain the principles of basic molecular biology methods and chemotaxonomic ones, propose the procedure for the solving of real problem, interpret the results.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid.
  • Fragmentation of DNA molecules. Restriction enzymes.
  • Analysis of nucleic acids using electromigration methods.
  • Analysis of nucleic acids using chromatographic methods.
  • Sequence analysis. Spectrophotometric analysis of nucleic acids.
  • Hybridisation.
  • Polymerase chain reaction (PCR).
  • Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components.
  • Principes of quantitative analysis.
  • Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
    recommended literature
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
Teaching methods
The teaching methods make use of presentations on stencils. Presentations of the results of own analysis of real samples are included and used for the explanation of problems. The course is followed up by a practical course.
Assessment methods
The course is closed with a test. The test contains 30 questions, which must be answered briefly. Each student must correctly answered 20 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi6721 Special Methods of Microorganisms Analysis I.

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Aleš Kovařík, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Prerequisites (in Czech)
Výuka je určena studentům mikrobiologie, případně studentům jiných oborů. Maximální počet studentů v praktiku je 12 (přednost mají posluchači mikrobiologie). Předpokladem zapsání předmětu je zkouška z molekulární biologie(Bi4020).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Methods of microorganisms analysis. Isolation of nucleic acids. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Syllabus
  • Methods of microorganisms analysis. Isolation of nucleic acid. Fragmentation of DNA molecules. Restriction enzymes. Analysis of nucleic acids using electromigration methods. Analysis of nucleic acids using chromatographic methods. Sequence analysis. Spectrophotometric analysis of nucleic acids. Hybridisation. Polymerase chain reaction (PCR). Methods of protein analysis. Chromatographic determination of microbial metabolites and bacterial cell components. Principes of quantitative analysis. Immunomagnetic and affinity based separation techniques in microorganisms diagnostics.
Literature
  • G. C. Saunders, H. C. Parkers. Analytical Molecular Biology, RSC, Cambridge 1999.
  • Rozsypal S. a kol. Úvod do molekulární biologie, IV, 3 vydání, Brno 2002
  • 1. Rickwood D., Hames B.D. (eds.) Gel electrophoresis of nucleic acids. 2 nd ed. IRL Press, Oxford 1990. 2. Sinden R.R. DNA structure and fuction. Academic Press, San Diego 1994. 3. Churáček J. a kol. Analytická separace látek. SNTL, Praha 1990.
Assessment methods (in Czech)
Součástí předmětu jsou laboratorní cvičení. Předmět je ukončen zkouškou, laboratorní cvičení zápočtem.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)