Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 8:00–9:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-BI) || PROGRAM(B-MBB) || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Mgr. Jarmila Navrátilová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 8:00–9:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-BI) || PROGRAM(B-MBB) || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/335
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-BI) || PROGRAM(B-MBB) || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/335
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6401 Bachelor Thesis II || Bi6491 Bachelor Thesis LGMD II || Bi6122 Bachelor thesis HB II || Bi1041 Introd. to Comp. Biology I || Bi6005 Bc. thesis II || Bi6006 Bc. thesis anim. phys. II || Bi6007 Bc. thesis || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 prace doma
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of Special biology || Bi1041 Introd. to Comp. Biology I || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/335
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of Special biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
Syllabus
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
Literature
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test followed by interview. At least 50% of correct answers are required to pass the test. During the interview, students are expexted to answer 1 - 2 questions of examinator. The questions deal with isues described during the course.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Thu 12:00–13:50 B11/235
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of Special biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Evaluation is based on written test followed by oral examination. At least 50% of correct answers are required in written test to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Thu 12:00–13:50 B11/305
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of Special biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Thu 12:00–13:50 B11/305
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of Special biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/305
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of General biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/305
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of General biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2013
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 16. 9. to Fri 6. 12. Thu 12:00–13:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6089 Bachelor state exam of Anthropogenetics || Bi6082 Bachelor state exam of General biology || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix, how nerve-, muscle- and immune systems function and what are main principles of intramolecular traficking. This provide students with capabilites to judge general rules of eukarytic cells functioning in multicellular organisms.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G, cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa,signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, collagen, hyaluronic acid, proteoglycans, cadherins, laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses,action potential, structure of channel proteins, membrane permeability, neuromuscular connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6. Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymphokines,monokines, interferons, TNF, antigen processing, MHCI and MHCII. 7. Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labeling by ubiquitin, proteasome, other ways of protein labeling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic reticulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesicular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 12:00–13:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Thu 12:00–13:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor sate exam of Cellular and molecular diagnostics || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 11:00–12:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 11:00–12:50 BR3
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Thu 8:00–9:50 BR3
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information on the most recent development within the field of moleular biology of eukaryotic cell. Main objectives can be summarized as follows" to understand molecular mechanism of cell cycle and principles of its regulation; to learn structure of DNA in chromatin; to understand principles of signal transduction; to delineate the major differences between healthy and cancer cells; to learn molecular principles of programmed cell death; to understand the ways eukaryotic cells communicate with neighbour cells and extracellular matrix; to learn principles of intramolecular traficking
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Assessment methods
Written tests are required to pass the examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 9:00–10:50 BR2
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Tue 11:00–12:50 BR2
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Thu 8:00–9:50 B1,01004
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Timetable
Wed 9:00–10:50 B1,01004
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students will acquire general knowledge on recent developments within the field of moleular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction. In addition, he/she should be able to delineate the major differences between healthy and cancer cells, to learn molecular principles of programmed cell death, to understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and to learn principles of intramolecular traficking.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008, xxxiii, 12. ISBN 9780815341062. info
  • ALBERTS, Bruce. Essential cell biology. 2nd ed. New York: Garland science, 2004, xxi, 740. ISBN 0815334818. info
Teaching methods
Lectures including brief discussions.
Assessment methods
Examination is based on written test is required. At least 50% of correct answers are required to pass.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7090 Molecular biology of eukaryotes

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jan Šmarda, CSc. (lecturer)
doc. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Basic course of Molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide information about the most recent development within the field of moleular biology of eukaryotic cell. The most dynamic areas of the research are espetially in the focus as, for example, molecular mechanisms of cell cycle regulation, signal transduction and cancer. In addition, detail description of structure and function of the neural, muscle and immune systems is also included in the course.
Syllabus
  • 1. Molecular mechanisms of cell cycle regulation: phases, control points, cyclins, CDKs, principles of cell cycle regulation, cell cycle deregulation and tumor formation). 2. Cell signalling I: principles, signal types, receptor types. 3. Cell signalling II: SH2 domain, secondary messengers, JAK/STAT,MAP, Ras, Raf kinases, protein G,cAMP, Ca++ ions in signal transduction, PKA, PKC, PKCa, signals and cellular skeleton. 4. Cell-cell and cell-matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins,laminin, fibronectin, selectins, integrins, types cell-cell interactions. 5. Molecular mechanisms of neural and muscle systems: neural cells, synapses, action potential, structure of channel proteins, membrane permeability, neuro-muscle connections, thin and thick filaments, molecular mechanisms of muscle contraction, muscle cell differentiation, Myo protein. 6.Molecular immunology: hematopoietic cell differentiation, growth factors in hematopoiesis, lymfokins, monokins,interferons,TNF, antigen processing, MHCI and MHCII. 7.Molecular principles of tumor formation I: tumor cells, malignant transformation, roles of oncogenes, tumor suppressors and cell death regulators in tumor formation). 8. Molecular principles of tumor formation II: proto-oncogenes and their products, oncogene co-operation in carcinogenesis, apoptosis, clinical implications, viruses in malignant transformation. 9.Chromatin: nucleosomes, methods of chromatin analysis, importance of chromatin changes. 10. Yeast model system: live cycle, mating type determination, mating type switching, yeast artificial, chromosomes. 11. Regulated protein degradation: protein labelling by ubiquitin, proteasom, other ways of protein labelling for degradation, the role of ubiquitin system in disease pathogenesis. 12. Protein translocation: protein transfer to endoplasmic retikulum, signal sequence, chaperons, chaperonins, smooth ER and lipid synthesis, Golgi apparatus - organisation, function, metabolisms of lipids in GA, protein export from GA, mechanisms of vesikular transport, phagocytosis.
Literature
  • ALBERTS, Bruce. Essential cell biology : an introduction to the molecular biology of the cell. New York: Garland Publishing, 1998, xxii, 630. ISBN 0-8153-2045-0. info
  • ALBERTS, Bruce. Molecular biology of the cell. 3rd ed. New York: Garland Publishing, Inc., 1994, xliii, 129. ISBN 0-8153-1620-8. info
Assessment methods (in Czech)
Zkouška má dvě části: písemnou a ústní.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/labweb/prednask/predn.html
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)