Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Adéla Finstrlová, Ph.D. (assistant)
Mgr. Lenka Kosečková Micenková, Ph.D. (assistant)
Mgr. Martina Damborská (assistant)
Guaranteed by
Mgr. Ivana Mašlaňová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Ivana Mašlaňová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-MIK) || PROGRAM(N-BI) || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Adéla Finstrlová, Ph.D. (assistant)
Mgr. Lenka Kosečková Micenková, Ph.D. (assistant)
Mgr. Martina Damborská (assistant)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-MIK) || PROGRAM(N-BI) || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Adéla Finstrlová, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && (PROGRAM(N-MBG) || PROGRAM(N-LGM) || PROGRAM(N-MIK) || PROGRAM(N-BI) || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Adéla Finstrlová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6401 Bachelor Thesis II || Bi6491 Bachelor Thesis LGMD II || Bi6122 Bachelor thesis HB II || Bi1041 Introd. to Comp. Biology I || Bi6005 Bc. thesis II || Bi6006 Bc. thesis anim. phys. II || Bi6007 Bc. thesis || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Mgr. Adéla Finstrlová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 prace doma
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of Special biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of Special biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of Special biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Wed 9:00–10:50 B11/305
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of Special biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to provide students with information on the storage of genetic information for prokaryotes, its implementation, changes and vertical and horizontal transmission. Students should understand the basic terminology of bacterial genetics, genome structure, chromosome replication and plasmids, the use of transposons to analyze genomes and create mutations and mechanisms of evolution of bacteria.
Learning outcomes
Student will be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
    not specified
  • SNYDER, Larry. Molecular genetics of bacteria. 4th ed. Washington, DC: ASM Press, 2013, xx, 707. ISBN 9781555816278. info
  • SNUSTAD, D. Peter and Michael J. SIMMONS. Genetika. Translated by Jiřina Relichová. Druhé, aktualizované vydá. Brno: Masarykova univerzita, 2017, xix, 844. ISBN 9788021086135. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. Ivana Mašlaňová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Wed 9:00–10:50 B11/306
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of Special biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 8:00–9:50 B11/235
Prerequisites
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || Bi6088 Bc. state exam Klin.Gen.Diagn. || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 13:00–14:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2013
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 16. 9. to Fri 6. 12. Tue 11:00–12:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able explain how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able to explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizontal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
    recommended literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with teacher comments. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 11:00–12:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 11:00–12:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor sate exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 15:00–16:50 B11/306
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 9:00–10:50 BR3
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6082 Bakalářská státní závěrečná zkouška z Obecné biologie || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 11:00–12:50 BR3
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6082 Bakalářská státní závěrečná zkouška z Obecné biologie || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives can be summarized as follows: Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular evolution of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • Lewin B.: Genes V-VII, Oxford University Press, Oxford, New York Tokyo 1994-2000.
Assessment methods
Oral exam
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 11:00–12:50 BR3
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 9:00–10:50 BR2
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 13:00–14:50 B1,01004
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Tue 12:00–13:50 B1,01004
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bachelor state exam of Molecular biology and genetics || Bi6082 Bachelor state exam of General biology || Bi6087 Bachelor state exam of Cellular and molecular diagnostics || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology ) && ( Bi6081 Bakalářská státní závěrečná zkouška z Molekulární biologie a genetiky || Bi6082 Bakalářská státní závěrečná zkouška z Obecné biologie || Bi6087 Bakalářská státní závěrečná zkouška z Buněčné a molekulární diagnostiky || SOUHLAS)
Going through basic lectures from Molecular Biology and/or Cellular and Molecular Biology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand how the genetic information of prokaryotes is maintained, changed and transferred vertically and horizontally. The students should be able explain the basic terms of bacterial genetics. structure and reproduction of bacterial chromosome, plasmid-borne functions, regulation of plasmid replication, genetics with transposons, the ways of horizotntal gene transfer in bacteria and molecular evolution processes of prokaryotic genomes.
Syllabus
  • Terminology of bacterial genetics
  • Structure and reproduction of bacterial chromosome
  • Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination)
  • F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens
  • Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F- x Hfr crosses)
  • Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages)
  • Transformation
  • Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair
  • Restriction and modification of DNA, RM systems of bacteria
  • Molecular evolution of prokaryotic genomes.
Literature
  • LEWIN, Benjamin. Genes VIII. 1st ed. Upper Saddle River: Pearson Education, 2004, xxi, 1027. ISBN 0131239244. info
  • Microbial evolution : gene establishment, survival, and exchange. Edited by Robert V. Miller - Martin J. Day. Washington, D.C.: ASM Press, 2004, xiv, 374. ISBN 1555812716. info
  • SNYDER, Larry and Wendy CHAMPNESS. Molecular genetics of bacteria. 2nd ed. Washington: ASM Press, 2003, xvi, 566. ISBN 155581204X. info
  • JOSET, Francois. Prokaryotic genetics : genome organization, transfer and plasticity. 1st pub. Oxford: Blackwell scientific publications, 1993, 454 s. ISBN 0632027282. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated accordindg to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Visiting of lectures is not obligatory. Oral examination follows thewritten test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Absolvování základní přednášky z Molekulární biologie nebo Buněčné a molekulární biologie
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.
Syllabus
  • 1. Struktura prokaryotického genomu, jeho základní složky a jejich vzájemné interakce. 2. Bakteriální chromozom, základní typy plazmidů 3. Mobilní elementy prokaryot 4. Mutace 5. TRanspozonová mutageneze 6. Restrikce a modifikace 7. Konjugace 8. Tranformace 9. Trandukce 10. Metody analýzy genomu 11. Plasticita prokaryotického genomu 12. Regulace genové exprese
Literature
  • Joset F. and Guespin-Michel J.: Prokaryotic genetics, Blackwell Science, Cambridge, 1994.
Assessment methods (in Czech)
Výuka probíhá formou přednášek, zkouška ústní s písemnou přípravou
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.sci.muni.cz/kgmb/Student/7120.htm
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)