Bi7201c Genomics - practice
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Vojtěch Didi, Ph.D. (seminar tutor)
Ing. Vendula Hrdinová (seminar tutor)
Ing. Hana Zimová (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi7201c/Bi7201c_beh01: No timetable has been entered into IS. T. Dobisová, J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová
Bi7201c/Bi7201c_beh02: No timetable has been entered into IS. T. Dobisová, J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová
Bi7201c/Bi7201c_beh03: No timetable has been entered into IS. T. Dobisová, J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová - Prerequisites
- NOW( Bi7201 Genomics ) || NOW( CG020 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Ing. Vendula Hrdinová (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/Bi7201c_01: No timetable has been entered into IS.
Bi7201c/Bi7201c_02: No timetable has been entered into IS.
Bi7201c/Bi7201c_03: No timetable has been entered into IS. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Mgr. Jakub Horák, Dr. rer. nat. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/Bi7201c_beh_01: No timetable has been entered into IS. J. Hejátko, J. Horák, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová
Bi7201c/Bi7201c_beh_02: No timetable has been entered into IS. J. Hejátko, J. Horák, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová
Bi7201c/Bi7201c_beh_03: No timetable has been entered into IS. J. Hejátko, J. Horák, H. Konečná, A. Kuderová, E. Nejedlá, M. Pernisová - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Mgr. Jakub Horák, Dr. rer. nat. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/beh_01: No timetable has been entered into IS.
Bi7201c/beh_02: No timetable has been entered into IS.
Bi7201c/beh_03: No timetable has been entered into IS. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/Beh_01: No timetable has been entered into IS.
Bi7201c/Beh_02: No timetable has been entered into IS.
Bi7201c/Beh_03: No timetable has been entered into IS. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Student will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/1_beh: No timetable has been entered into IS. J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, E. Paděrová, M. Pernisová
Bi7201c/2_beh: No timetable has been entered into IS. J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, E. Paděrová, M. Pernisová
Bi7201c/3_beh: No timetable has been entered into IS. J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, E. Paděrová, M. Pernisová - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Student will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment.
- Syllabus
- See the teaching materials on our www pages (http://www.sci.muni.cz/fgp).
- Assessment methods (in Czech)
- Na konci cvičení bude udělen zápočet a to na základě včasného dodání všech požadovaných protokolů a absolvování všěech požadovaných testů.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Nagavalli Subbanna Kiran, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Timetable of Seminar Groups
- Bi7201c/beh_1: No timetable has been entered into IS. J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, E. Paděrová, M. Pernisová
Bi7201c/beh_2: No timetable has been entered into IS. J. Hejátko, H. Konečná, A. Kuderová, E. Nejedlá, E. Paděrová, M. Pernisová - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Student will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment.
- Syllabus
- See the teaching materials on our www pages (http://www.sci.muni.cz/fgp).
- Assessment methods (in Czech)
- Na konci cvičení bude udělen zápočet a to na základě včasného dodání všech požadovaných protokolů a absolvování všěech požadovaných testů.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Nagavalli Subbanna Kiran, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Prerequisites (in Czech)
- NOW( Bi7201 Genomics )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- Biophysics (programme PřF, N-FY)
- Biochemistry (programme PřF, N-BCH)
- Environmental Chemistry (programme PřF, N-CH)
- Molecular Biology and Genetics (programme PřF, N-BI)
- General Biology (programme PřF, N-BI)
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 0/3/0. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Tereza Dobisová, Ph.D. (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Mgr. Jakub Horák, Dr. rer. nat. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end ofg the course, students will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment. At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
- Syllabus
- Design of oligonucleotides sequence
- Oligonucleotides synthesis
- Analysis of gene expression using transcriptional fusion
- Isolation of plant genomic DNA
- Identificatio of insertional mutant using PCR
- Working woth databasis of molecular-biology informations
- Transfer of DNA on membrane, preparation of labeled probe and hybridization
- Detection of hybridized probe
- Fragmentation analysis
- Literature
- Teaching methods
- Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
- Assessment methods
- The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Bi7201c Genomics - practice
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (seminar tutor)
Nagavalli Subbanna Kiran, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D. - Prerequisites
- NOW( Bi7201 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Student will acquire practical experience with basic methods of functional genomics, i.e. particularly with the identification of sequence specific insertional mutants identification: From the primer design, (primers) DNA synthesis, PCR, PCR product identification on agarose gels and confirmation of the PCR specificity using Southern blot hybridization with labeled probe till the fragmentation analysis using automated sequencing machine. The work is organized in groups of two students each, allowing thus self-dependent experiment planning and accomplishment.
- Syllabus
- See the teaching materials on our www pages (http://www.sci.muni.cz/fgp).
- Assessment methods (in Czech)
- Na konci cvičení bude udělen zápočet a to na základě včasného dodání všech požadovaných protokolů a absolvování všěech požadovaných testů.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
- Enrolment Statistics (recent)