Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2011
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 12:00–13:50 Bpt,01013
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2010
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 12:00–13:50 Bpt,01013
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2009
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 12:00–13:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from Molecular biology (Bi4020) and General microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Genetics and principles of classical and reversion genetics. Genetic terminology (mutants, genotypes, phenotypes). Genomes of bacteria, archea and eukaryotic microorganisms. Transfer of genes between bakteria (conjugation, transduction and transformation). Phage mutants and constrution of phage genetic map. Natural transformation and DNA transfer into cells during transformation. Characterisation of restriction modification systems. Mobile genetic elements and transposition. Regulation of gene expression in prokaryots (levels of regulation, enzymatic induction and repression, catabolic repression, negative and positive regulation of operon, attenuation, classification of regulators). New era in genetics - sequencing. Bioinformatics and proteomics.
At the end of this course, student should be able to understand genetic terminology and genetic maps, explain different types of natural gene transfer among microorganisms and thein importace and understand principles of regulation of gene expression u procaryot.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Assessment methods
The course is enclosed by test and oral exam.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2008
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–11:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2007
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 13:00–14:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2006
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 8:00–9:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2005
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 15:00–16:50 Bpt,01013
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Domains Bacteria, Archea, Eucarya, their characteristics from the point of wiev of genetic analysis, universal phylogenetic tree. Mutations and isolation of mutants. The use of bacterial mutants in testing of mutagenic agents. Genetic terminology. Phenotypes often used in bacterial genetics. Plasmids and conjugation. Bacteriophages and transduction. Natural compentency and transformation. Restriction and modification of DNA. Transposons and transposition. Sequencing of whole genomes and analysis of sequences. Gene expression and regulation.
Syllabus
  • Microorganisms in the living system - domains Bakteria, Archea, Eucarya. Universal phylogenetic tree of Woese. Clasical genetic analysis. Reversed genetics. Bioinformatics. Characteristics of microorganisms suitable for genetic experiments. Spontaneous and induced mutations. The use of bacterial mutants in the testing of mutagenic agents. Genetic terminology. Phenotypes mostly used in bacterial genetics. Conjugation and conjugatible plasmids. The conjugation machinery. Mobilisation of plasmids. Formation of Hfr strains. Transfer of DNA from an Hfr to another cell, transconjugants. Mapping genes on the chromosome using Hfr crosses. Fand R plasmids. Virulent and temperate bacteriophages. General transduction. Lysogenic state and lysis of bacterial cell. Specialized transduction. Genetic analysis of phages. Construction of genetic map. Genetic transformation. Natural competency. The machinery of naturally transformable cells. Restriction and modification systems. Transposition. IS elements, composite and non-composite transposons. Mechanisms of transposition. Sequencing of whole genomes and analysis of sequences. Genome composition of archeon Methanococcus janaschii. Molecular basis of life-adaptation in extreme conditions. Regulation of gene expression of procaryots. Enzyme induction. Enzyme repression. Catabolic repression. Lactose operon and the other operons of E. coli. Atenuation. Antisens RNA. Regulation of translation.
Literature
  • Snyders L.S., Champness W. ed., Molecular Genetics of Bacteria, ASM Washington, 1997
  • Rosypal S., Molekulární biologie, Díl čtvrtý, Brno, 2001
  • Trun N., Trempy J., Fundamental Bacterial Genetics, Blackwell Publishing, UK, 2004
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2004
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2003
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2025

The course is not taught in Spring 2025

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2024

The course is not taught in Spring 2024

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2023

The course is not taught in Spring 2023

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2022

The course is not taught in Spring 2022

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2021

The course is not taught in Spring 2021

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2020

The course is not taught in Spring 2020

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2019

The course is not taught in Spring 2019

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
spring 2018

The course is not taught in spring 2018

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2017

The course is not taught in Spring 2017

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2016

The course is not taught in Spring 2016

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2015

The course is not taught in Spring 2015

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archaea and Eukarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and uninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and construction of phage genetic map.
  • Natural transformation and its importance. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryotes. Levels of regulation. Enzymatic induction, enzymatic repression, catabolite repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a oral exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the oral exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2013

The course is not taught in Spring 2013

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2012

The course is not taught in Spring 2012

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
General note: Předmět není počínaje JS 2012 vypisován; obsahovou náhradou je Bi7120 Molekulární biologie prokaryot (v PS).
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of Microorganisms

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011.

Bi8021 Genetics of Microorganisms

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is destined for students interested in acquiring information about the mechanisms of natural transfer of genes among microorganisms and about genetic analysis of microorganisms. Courses in Molecular Biology (Bi4020) and General Microbiology (Bi4090) are required as prerequisites.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students should be able to understand genetic terminology, the different types of natural gene transfer among microorganisms, the importance of mutants for construction of genetic maps, and principles of regulation of gene expression in prokaryotes. They will also understand genetic terminology.
Syllabus
  • Definition of genetics of microorganisms. The impact of bacterial genetics for molecular biology. Genomes of domains Bacteria, Archea and Eucarya. Classical and reversion genetics.
  • Main characteristics of bacteria suitable for genetic analysis. Genetic terminology.
  • Mutations and mutant isolation. Phenotypes used in bacterial genetics.
  • Use of bacterial mutants – Ames test and SOS Chromotest.
  • Replication, transcription and translation of archeal genomes.
  • Transfer of genes between microorganisms. Plasmids, F plasmids and mechanism of conjugation. Hfr strains and transfer of chromosomal genes.
  • Interrupted and noninterrupted conjugation and construction of genetic maps. F‘ and R‘ plasmids, mobilised plasmids. General recombination during conjugation.
  • Virulent and temperate bacteriophages. General transduction. Bacteriophage lambda, its lifecycle.
  • Specialised transduction. Phage mutants and constrution of phage genetic map.
  • Natural transformation and its importace. Different kinds of DNA transfer into cells during transformation.
  • Restriction and modification of DNA, characterisation of restriction modification systems.
  • Transposition. IS elements and transposons, their structure and importance in gene transfer. Conjugative transposons.
  • Regulation of gene expression in prokaryots. Levels of regulation. Enzymatic induction, enzymatic repression, catabolic repression. Negative and positive regulation of operon.
  • Attenuation. Other kinds of regulation of gene expression in bacteria. Classification of regulators.
  • New era in genetics - sequencing. Bioinformatics and proteomics.
Literature
  • Rosypal, S. Introduction into molecular biology (Úvod do molekulární biologie). Brno : Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006. 290 s. ISBN 80-902562-5-2.
  • Trun N., Trempy J., Fundamentals Bacterial Genetics, Blackwell Science Ltd 2003, ISBN 978-0632044481
Teaching methods
Presentations on stencils and in PowerPoint are used in the instruction. Presentations of real results of analysis are included and used for the explanation of problems.
Assessment methods
The course is closed with a test and a spoken exam. The test contains 50 questions which must be answered briefly. Each student who has correctly answered 30 questions qualifies for the spoken exam. The student is given 2 questions. The overall result of the exam is evaluated by the examiner.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8021 Genetics of microorgansims

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The course is for students which are interested in informations about the genetic analysis of microorganisms. The prerequisits are courses from biochemistry and molecular biology (Bi4020) and general microbiology (Bi4090)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Syllabus
  • The impact of bacterial genetics for molecular biology. Bacteria, archea and other microorganisms; their main characteristics suitable for genetic analysis. Mutations and phenotypes used in bacterial genetics. Principles of mutant isolations. Characterisation of mutants. Insertion and transposition elements. Transposone mutagenesis. Gene transfer in Gram negative bacteria. Gene transfer in Gram positive bacteria. Gene transfer in Archea. Characterisation of selected species of bacteria and archea.
Literature
  • 1. Snyder L. and Champness W. (1997) Molecular Genetics of Bacteria. ASM Press. Washington, D.C. 2. Bruijn F.J., Lupski J.R. and Weinstock G.M. (1998) Bacterial Genomes. ITP Thomson Science.
Assessment methods (in Czech)
Výuka předmětu je ukončena zkouškou.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (recent)