Bi8090 Gene engineering

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. Mgr. Petr Beneš, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Petr Beneš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

Bi8090 Gene engineering

Faculty of Science
Spring 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Thu 16:00–17:50 online_B1
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • 1. What is genetic engineering, Content of the lectures.
  • 2.Mutagenesis in vitro,
  • 3.Optimalizatiopn of gene expresion in heterologous organisms.
  • 4.Gene clonig in G+ bacteria, (Bacillus, Streptomyces).
  • 5. Vectors for gene cloning to eukaryotic cells. Selection markers. Electroporation, biolistic method, liposomes,transfectiopn.
  • 6.Gene cloning in yeasts.
  • 7. Preparation of transgenic plants, properties of transgenic plants, Ti-vectors.
  • 8. Gene cloning in animal cell and its application.
  • 9.Gene therapy, in vitro a in vivo.
  • 10.Genetically modified organisms as producers of farmaceutics and other important substances.
  • 11. Chromosome engineering. CRISPR/Cas.
  • 12. Risks of preparation of transgenic organisms, rules of work safety with transgenic organisms. Legislation on the handling of GMOs.
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
    not specified
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Předmět se doporučuje zapsat v 2. nebo 4. semestru.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
    not specified
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
spring 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
prof. Mgr. Petr Beneš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Learning outcomes
Students will be able to understand and explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
    not specified
  • Microbial biotechnology : principles and applications. Edited by Y. K. Lee. 3rd ed. New Jersey: World Scientific, 2013, xvi, 877. ISBN 9789814366823. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students will be able to explain the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
    recommended literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers which are explained and complemented with live commentaries. All the presentations are available in IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 16:00–17:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 8:00–9:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 8:00–9:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 11:00–12:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 11:00–12:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology, and it is recommended to go through the lectures from Molecular Biology of prokaryotes, Molecular Biology of viruses, Molecular biology of eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of this course students understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and practical application
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods
oral exam
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 8:00–9:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 17:00–18:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 9:00–10:50 BR3
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Fri 13:00–14:50 B1,01004
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8090 Gene engineering

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Absolvování přednášek: Metody molekulární biologie, Molekulární biologie virů, prokaryot a eukaryot.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
Posluchači jsou seznámeni s využitím metod molekulární biologie při genových modifikacích organismů a jejich využitím ve výzkumu a v praxi.
Syllabus
  • 1. Definice genového inženýrství, historie jeho vzniku, jeho význam a perspektivy.2. Mutageneze in vitro, cílené změny genetického materiálu, náhodná mutageneze, mutageneze pomocí mutagenních oligonukleotidů, kazetová mutageneze, využití supresorových tRNA. Základy proteinového inženýrství. 3. Optimalizace exprese klonovaných genů, faktory ovlivňující expresi genů v cizorodých hostitelích. 4.Klonování genů v grampozitivních organizmech, možnosti jeho využití (Bacillus, Streptomyces). Způsoby přenosu cizích genů do eukaryotických buněk (mikroinjekce, elektroporace, transfekce, vektorové systémy, biolostické metody). 5. Obecná charakteristika vektorů pro přenos genů do eukaryot, selekční markery. 6.Klonování genů ve kvasinkách a jeho využití pro analýzu eukaryotického genomu. 7.Klonování genů v rostlinách a jeho využití. Přenos genů pomocí vektorů odvozených od Ti-plazmidu. 8.Klonování genů v živočišných buňkách.9. Přenos cizích genů do zárodečných buněk (vajíček, embryií) hmyzu, obojživelníků a savců. 9.Navozování cílených změn v genomu živočichů, jeho využití v základním výzkumu a v praxi. 11.Příprava transgenních organismů (transgenoze). 12.Genové terapie, hlavní strategie genové terapie in vitro a in vivo. 13.Využití metod rekombinantní DNA v zemědělství, průmyslu a zdravotnictví. Příprava farmakologicky významných látek v nepříbuzných hostitelích. Příprava látek s novými vlastnostmi (vakcíny, protilátky, enzymy). Klonování živočichů. Rizika přípravy transgenních organizmů, pravidla bezpečnosti práce s transgenními organizmy. Etické problémy související s mezidruhovým přenosem genů a přípravou transgenních organizmů.
Literature
  • Old, R.W., Primrose S.B. Principles of gene manipulation.
  • Blackwell Science, 1995. Oxford.
Assessment methods (in Czech)
Výuka probíhá formou přednášky, zkouška je ústní s písemnou přípravou.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)