Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2012
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 10:00–11:50 B11/235
- Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- Lecture will be given in 4-hour units
study materials will be available in IS
written exam - Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2011
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Timetable
- Tue 11:00–12:50 B11/235
- Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- written exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2010
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Timetable
- Wed 9:00–10:50 C02/211
- Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- Lecture will be given in 4-hour units
study materials will be available in IS
written exam - Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2009
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c). Students obtain general overview about proteomics and proteomic tools. They will be prepared for following practical proteomic coarses
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods
- Lecture will be given in 4-hour units
study materials will be available in IS
written exam - Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
The course is taught: every week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2008
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2008 bude výuka probíhat ve dnech 25.4., 2.5. a 9.5., vždy od 8.30 do 12.00 na UKB, Kamenice 5, budova A3, přednášková místnost v přízemí. Řádná zkouška proběhne písemně ve dvou termínech, 16.5. a 20.5., opravný termín pak 23.5., vždy v 9.00. Případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
The course is taught: every week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2007
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2007 bude výuka probíhat ve dnech 27.4., 4.5. a 11.5., vždy od 8.00 do 12.00 na UKB, Kamenice 5, budova A2, seminární místnost 2.11. Řádná zkouška proběhne písemně ve dvou termínech, 18.5. a 25.5., případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
The course is taught: every week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2006
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
- Teacher(s)
- prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer), prof. Mgr. Jan Preisler, Ph.D. (deputy)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc. - Prerequisites
- Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
- Syllabus
- Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Blokové praktikum, ukončeno (klasifikovaným) zápočtem
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2005
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
- Teacher(s)
- prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc. - Prerequisites
- Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
- Syllabus
- Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Blokové praktikum, ukončeno (klasifikovaným) zápočtem
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2004
- Extent and Intensity
- 1/2/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc. - Prerequisites
- Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36 - fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
- Syllabus
- Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Blokové praktikum, ukončeno (klasifikovaným) zápočtem
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2003
- Extent and Intensity
- 1/2/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc. - Prerequisites
- Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biophysics (programme PřF, M-FY)
- Biochemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, M-CH)
- Molecular Biology and Genetics (programme PřF, M-BI)
- General Biology (programme PřF, M-BI)
- Course objectives
- Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
- Syllabus
- Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Blokové praktikum, ukončeno (klasifikovaným) zápočtem
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
Bi8202 Proteomics - a basic course
Faculty of Sciencespring 2012 - acreditation
The information about the term spring 2012 - acreditation is not made public
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- Lecture will be given in 4-hour units
study materials will be available in IS
written exam - Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every other week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2011 - only for the accreditation
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- Lecture will be given in 4-hour units
study materials will be available in IS
written exam - Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every other week.
Bi8202 Proteomics - a basic course
Faculty of ScienceSpring 2008 - for the purpose of the accreditation
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer) - Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr. - Prerequisites
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 10 fields of study the course is directly associated with, display
- Course objectives
- This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
- Syllabus
- Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
- Literature
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Assessment methods (in Czech)
- Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2007 bude výuka probíhat ve dnech 27.4., 4.5. a 11.5., vždy od 8.00 do 12.00 na UKB, Kamenice 5, budova A2, seminární místnost 2.11. Řádná zkouška proběhne písemně ve dvou termínech, 18.5. a 25.5., případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (recent)