Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2012
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/235
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2011
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Timetable
Tue 11:00–12:50 B11/235
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2010
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
RNDr. Jana Buršíková (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Timetable
Wed 9:00–10:50 C02/211
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2009
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c). Students obtain general overview about proteomics and proteomic tools. They will be prepared for following practical proteomic coarses
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2008
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2008 bude výuka probíhat ve dnech 25.4., 2.5. a 9.5., vždy od 8.30 do 12.00 na UKB, Kamenice 5, budova A3, přednášková místnost v přízemí. Řádná zkouška proběhne písemně ve dvou termínech, 16.5. a 20.5., opravný termín pak 23.5., vždy v 9.00. Případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2007
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2007 bude výuka probíhat ve dnech 27.4., 4.5. a 11.5., vždy od 8.00 do 12.00 na UKB, Kamenice 5, budova A2, seminární místnost 2.11. Řádná zkouška proběhne písemně ve dvou termínech, 18.5. a 25.5., případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer), prof. Mgr. Jan Preisler, Ph.D. (deputy)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
Syllabus
  • Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
Syllabus
  • Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2004
Extent and Intensity
1/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
Syllabus
  • Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2003
Extent and Intensity
1/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4020 Molecular biology
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students will acquire a working knowledge of high throughput, high resolution techniques of protein separation and identification in complex biological samples. The techniques form a base for a global analysis of a protein complement of expressed genome - proteome. Further, the students will master basic techniques of protein structural biology, including production in bacterial cells of eukaryotic proteins as fusion proteins, and their subsequent single-step affinity chromatography purification, and structure-function characterization.
Syllabus
  • Introduction to proteome analysis. Methods of proteome analysis. Electrophoretic techniques - 1-D, 2-D PAGE, IEF. Mass spectrometry techniques - MALDI-TOF, ESI-MS. Proteome databases. Recombinant protein technology. DNA sequence analysis. Chromatography techniques. Effects of protein fusions. Image analysis.
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every other week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (Bi8202c) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems via individual tools of recent proteomics.
Assessment methods
Lecture will be given in 4-hour units
study materials will be available in IS
written exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every other week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202 Proteomics - a basic course

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
RNDr. Lubomír Janda, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Mgr. Ivana Urbánková (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Prerequisites
Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
This course offers introduction in proteomics and theoretical basis for successful completing of subsequent practical course (Bi8202c)
Syllabus
  • Introduction in proteomics Rekombinant proteins Characterization of protein function Protein structure Protein separation Image analysis of 2-D gels Analysis of proteins by mass spectrometry
Literature
  • Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
  • Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
  • Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
Assessment methods (in Czech)
Předmět bude vyučován blokově (3 bloky) a ukončen zkouškou. V jarním semestru 2007 bude výuka probíhat ve dnech 27.4., 4.5. a 11.5., vždy od 8.00 do 12.00 na UKB, Kamenice 5, budova A2, seminární místnost 2.11. Řádná zkouška proběhne písemně ve dvou termínech, 18.5. a 25.5., případné opravné termíny budou vypsány dle potřeby. Upozorňujeme na podmínku absolvování zkoušky pro možnost absolvování praktika z proteomiky.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.
  • Enrolment Statistics (recent)