Bi8202c Proteomics - practice

Faculty of Science
Spring 2012
Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (assistant)
Bc. Martina Válková (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8202c/Beh_01: No timetable has been entered into IS.
Bi8202c/Beh_02: No timetable has been entered into IS.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2011
Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
RNDr. Jana Buršíková (seminar tutor)
Danuše Fridrichová (seminar tutor)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (seminar tutor)
Bc. Martina Válková (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Bi8202c_01: No timetable has been entered into IS. J. Buršíková, R. Dopitová, D. Fridrichová, J. Hejátko, L. Janda, H. Konečná, J. Marek, Š. Michlíčková, E. Nejedlá, B. Pekárová, M. Válková, Z. Zdráhal
Bi8202c/Bi8202c_02: No timetable has been entered into IS. J. Buršíková, R. Dopitová, D. Fridrichová, J. Hejátko, L. Janda, H. Konečná, J. Marek, Š. Michlíčková, E. Nejedlá, B. Pekárová, M. Válková, Z. Zdráhal
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2010
Extent and Intensity
0/3. 3 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Jana Buršíková (seminar tutor)
Danuše Fridrichová (seminar tutor)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Bc. Martina Válková (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Bi8202c_beh_01: No timetable has been entered into IS.
Bi8202c/Bi8202c_beh_02: No timetable has been entered into IS.
Prerequisites
Succesful completion (passing the exam succesfully) of the course "Bi8202 Proteomics, a basic course" is obligatory to allow subscription of the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2009
Extent and Intensity
0/3. 4 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. David Potěšil, Ph.D. (seminar tutor)
Mgr. Markéta Žďárská, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Beh_01: No timetable has been entered into IS. R. Dopitová, J. Hejátko, L. Janda, H. Konečná, J. Marek, E. Nejedlá, E. Paděrová, D. Potěšil, Z. Zdráhal, M. Žďárská
Bi8202c/Beh_02: No timetable has been entered into IS. R. Dopitová, J. Hejátko, L. Janda, H. Konečná, J. Marek, E. Nejedlá, E. Paděrová, D. Potěšil, Z. Zdráhal, M. Žďárská
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2008
Extent and Intensity
0/3. 3 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Ivana Urbánková (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Beh_01: No timetable has been entered into IS.
Bi8202c/Beh_02: No timetable has been entered into IS.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Assessment methods (in Czech)
Cvičení budou proběhne ve dvou blocích a to od 26.5. - 30.5. a od 2.6. - 7.6. v prostorách pavilonu A2, UKB.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2007
Extent and Intensity
0/3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
RNDr. Lubomír Janda, Ph.D. (seminar tutor)
Bc. Martina Válková (assistant)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Ivana Urbánková (seminar tutor)
Danuše Fridrichová (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/01: No timetable has been entered into IS. J. Hejátko, H. Konečná, E. Nejedlá, Z. Zdráhal
Bi8202c/02: No timetable has been entered into IS. J. Hejátko, H. Konečná, E. Nejedlá, Z. Zdráhal
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Assessment methods (in Czech)
Předmět bude vyučován blokově (2 bloky). V jarním semestru 2007 bude výuka probíhat: 1. blok ve dnech 21.5. až 25.5. a 2. blok ve dnech 28.5. až 1.6.,výuka bude probíhat v laboratořích OFGP na UKB, Kamenice 5, budova A2. Do jednoho bloku se může přihlásit max. 12 studentů.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2006
Extent and Intensity
0/3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Mgr. Pavel Mazura, Ph.D. (seminar tutor)
Mgr. Ivana Urbánková (seminar tutor)
Bc. Martina Válková (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Timetable of Seminar Groups
Bi8202c/Beh_1: No timetable has been entered into IS. R. Dopitová, J. Hejátko, H. Konečná, P. Mazura, E. Nejedlá, E. Paděrová, I. Urbánková, M. Válková, Z. Zdráhal
Bi8202c/Beh_2: No timetable has been entered into IS. R. Dopitová, J. Hejátko, H. Konečná, P. Mazura, E. Nejedlá, E. Paděrová, I. Urbánková, M. Válková, Z. Zdráhal
Prerequisites
A prerequisite for a successful passing out a course is knowledge of basics of biochemistry and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
In the course the students will be educated in the basics of the experimental approaches recently used in the studies of the function of the sum of all proteins of the respective organism (proteome), but on the other hand also to the study of the features of individual proteins. Students will practice the methods of protein expression and purification in heterologous expression systems (E. coli), ranging from the recombinant DNA isolation and characterization, through the protein isolation, purification and purity analysis using SDS-PAGE and image analysis to the structural and functional analysis of purified proteins. Further there will be performed total protein isolation from the model organism Arabidopsis thaliana and students will be educated in the basic approaches of the proteome analysis using 2D electrophoresis and its evaluation using image analysis. It will be followed by the practical demonstration of the basics of the protein identification using MS spectrometry and the basics of the work with protein databases. All the above mentioned methods and methodological approaches are used in the Laboratory of Plant Molecular Physiology used for the solution of real biological and biochemical problems by the staff and students of the laboratory that will participate on the teaching of the course.
Assessment methods (in Czech)
Pro úspěšné absolvování kurzu a udělení zápočtu bude požadováno splnění všech studijních povinností a to nejpozději v POSLEDNÍ DEN konání daného běhu (tedy vždy v pátek). Jedná se především o absolvování několika písemných testů a odevzdání VŠECH požadovaných protokolů.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2005
Extent and Intensity
0/3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Mgr. Radka Dopitová, Ph.D. (assistant)
Mgr. Pavel Mazura, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught in blocks.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
Main objective of the course is to understand the basic approaches of the proteomics, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught in blocks.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.

Bi8202c Proteomics - practice

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Petra Borkovcová, Ph.D. (assistant)
Mgr. Pavlína Váňová (assistant)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught in blocks.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation.
  • Enrolment Statistics (recent)